利用多能干细胞评估神经发育毒性的研究进展
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摘要
<正>诱导性多能干细胞(induced pluripotent stem cells,iPSCs)是通过诱导成熟的体细胞表达特定基因得到的与胚胎干细胞(embryonic stem cells,ESCs)类似的细胞类型[1]。i PSCs和ESCs在体外均具有无限增殖和多向分化的能力,两者在药物筛选、细胞治疗等方面都发挥着重要作用,然而iPSCs来源广泛,取材方便,其获取可以避免医学伦理问题。传统的发育毒性研究方法不仅需要大量的实验动物,而且实验周期长,操作繁琐,还存在种属差异。1981年,
引文
[1]Takahashi K,Yamanaka S.Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.Cell,2006,126(4):663-676.
[2]Evans MJ,Kaufman MH.Establishment in culture of pluripotential cells from mouse embryos.Nature,1981,292(5819):154-156.
[3]Spielmann H,Pohl I,Doring B,et al.The embryonic stem cell test(EST),an in vitro embryotoxicity test using two permanent mouse cell lines:3T3 fibroblasts and embryonic stem cells.In Vitro Toxicol,1997,10(1):119-127.
[4]Aikawa N,Kunisato A,Nagao K,et al.Detection of thalidomide embryotoxicity by in vitro embryotoxicity testing based on human iPScells.J Pharmaco Sci,2014,124(2):201-207.
[5]Fang H,Zhi Y,Yu Z,et al.The embryonic toxicity evaluation of deoxynivalenol(DON)by murine embryonic stem cell test and human embryonic stem cell test models.Food Control,2018,86:234-240.
[6]Luo S,Fang HQ,Yang H,et al.Comparison of embryotoxicity of di(2-ethylhexyl)phthalate using mouse and human embryonic stem cell test models in vitro.Chin J Prev Med,2016,50(7):645-651.(in Chinese)罗莎,方海琴,杨辉,等.两种胚胎干细胞实验模型评价邻苯二甲酸(2-乙基己基)酯胚胎发育毒性的比较研究.中华预防医学杂志,2016,50(7):645-651.
[7]Rice D,Barone SJ.Critical periods of vulnerability for the developing nervous system:evidence from humans and animal models.Environ Health Perspect,2000,108 Suppl 3:511-533.
[8]Kraft AD,Aschner M,Cory-Slechta DA,et al.Unmasking silent neurotoxicity following developmental exposure to environmental toxicants.Neurotoxicol Teratol,2016,55:38-44.
[9]Grandjean P,Landrigan PJ.Neurobehavioural effects of developmental toxicity.Lancet Neurol,2014,13(3):330-338.
[10]Yi H,Xie B,Liu B,et al.Derivation and identification of motor neurons from human urine-derived induced pluripotent stem cells.Stem Cells Int,2018,2018:3628578.
[11]Santos DP,Kiskinis E.Generation of spinal motor neurons from human pluripotent stem cells.Methods Mol Biol,2017,1538:53-66.
[12]Datta I,Ganapathy K,Tattikota SM,et al.Directed differentiation of human embryonic stem cell-line HUES9 to dopaminergic neurons in a serum-free defined culture niche.Cell Biol Int,2013,37(1):54-64.
[13]Kawasaki H,Mizuseki K,Nishikawa S,et al.Induction of midbrain dopaminergic neurons from ES cells by stromal cell-derived inducing activity.Neuron,2000,28(1):31-40.
[14]Hu Y,Qu ZY,Cao SY,et al.Directed differentiation of basal forebrain cholinergic neurons from human pluripotent stem cells.J Neurosci Methods,2016,266:42-49.
[15]Vazin T,Ball K A,Lu H,et al.Efficient derivation of cortical glutamatergic neurons from human pluripotent stem cells:a model system to study neurotoxicity in Alzheimer's disease.Neurobiol Dis,2014,62:62-72.
[16]Pei Y,Peng J,Behl M,et al.Comparative neurotoxicity screening in human iPSC-derived neural stem cells,neurons and astrocytes.Brain Res,2016,1638(Pt A):57-73.
[17]Bian J,Zheng J,Li S,et al.Sequential differentiation of embryonic stem cells into neural epithelial-like stem cells and oligodendrocyte progenitor cells.PLoS One,2016,11(5):e155227.
[18]Arber C,Precious SV,Cambray S,et al.Activin A directs striatal projection neuron differentiation of human pluripotent stem cells.Development,2015,142(7):1375-1386.
[19]Reichman S,Slembrouck A,Gagliardi G,et al.Generation of storable retinal organoids and retinal pigmented epithelium from adherent human iPS cells in Xeno-free and feeder-free conditions.Stem Cells,2017,35(5):1176-1188.
[20]Matsuoka AJ,Morrissey ZD,Zhang C,et al.Directed differentiation of human embryonic stem cells toward placode-derived spiral ganglion-like sensory neurons.Stem Cells Transl Med,2017,6(3):923-936.
[21]Pamies D,Barreras P,Block K,et al.A human brain microphysiological system derived from induced pluripotent stem cells to study neurological diseases and toxicity.ALTEX,2017,34(3):362-376.
[22]Schwartz MP,Hou Z,Propson NE,et al.Human pluripotent stem cell-derived neural constructs for predicting neural toxicity.Proc Natl Acad Sci U S A,2015,112(40):12516-12521.
[23]Druwe I,Freudenrich TM,Wallace K,et al.Sensitivity of neuroprogenitor cells to chemical-induced apoptosis using a multiplexed assay suitable for high-throughput screening.Toxicology,2015,333:14-24.
[24]Tiwari SK,Agarwal S,Chauhan LK,et al.Bisphenol-A impairs myelination potential during development in the hippocampus of the rat brain.Mol Neurobiol,2015,51(3):1395-1416.
[25]Visan A,Hayess K,Sittner D,et al.Neural differentiation of mouse embryonic stem cells as a tool to assess developmental neurotoxicity in vitro.Neurotoxicology,2012,33(5):1135-1146.
[26]Guan S,Xu J,Guo Y,et al.Pyrroloquinoline quinone against glutamate-induced neurotoxicity in cultured neural stem and progenitor cells.Int J Dev Neurosci,2015,42:37-45.
[27]Augustyniak J,Lenart J,Zychowicz M,et al.Sensitivity of hiPSC-derived neural stem cells(NSC)to Pyrroloquinoline quinone depends on their developmental stage.Toxicol In Vitro,2017,45(Pt 3):434-444.
[28]Menzner AK,Gilbert DF.A protocol for in vitro High-throughput chemical susceptibility screening in differentiating NT2 stem cells.Methods Mol Biol,2017,1601:61-70.
[29]Inohana M,Eguchi A,Nakamura M,et al.Developmental exposure to aluminum chloride irreversibly affects postnatal hippocampal neurogenesis involving multiple functions in mice.Toxicological Sci,2018,164(1):264-277.
[30]Yang D,Lein PJ.Polychlorinated biphenyls increase apoptosis in the developing rat brain.Curr Neurobiol,2010,1(1):70-76.
[31]Mori H,Sasaki G,Nishikawa M,et al.Effects of subcytotoxic cadmium on morphology of glial fibrillary acidic protein network in astrocytes derived from murine neural stem/progenitor cells.Environ Toxicol Pharmacol,2015,40(2):639-644.
[32]Parsons-White AB,Spitzer N.Environmentally relevant manganese overexposure alters neural cell morphology and differentiation in vitro.Toxicol In Vitro,2018,50:22-28.
[33]Zimmer B,Lee G,Balmer NV,et al.Evaluation of developmental toxicants and signaling pathways in a functional test based on the migration of human neural crest cells.Environ Health Perspect,2012,120(8):1116-1122.
[34]Nyffeler J,Karreman C,Leisner H,et al.Design of a high-throughput human neural crest cell migration assay to indicate potential developmental toxicants.ALTEX,2017,34(1):75-94.
[35]Ryan KR,Sirenko O,Parham F,et al.Neurite outgrowth in human induced pluripotent stem cell-derived neurons as a high-throughput screen for developmental neurotoxicity or neurotoxicity.Neurotoxicology,2016,53:271-281.
[36]Rai NK,Ashok A,Rai A,et al.Exposure to As,Cd and Pb-mixture impairs myelin and axon development in rat brain,optic nerve and retina.Toxicol Appl Pharmacol,2013,273(2):242-258.
[37]Barateiro A,Fernandes A.Temporal oligodendrocyte lineage progression:in vitro models of proliferation,differentiation and myelination.Biochim Biophys Acta,2014,1843(9):1917-1929.
[38]Yl?-Outinen L,Heikkil?J,Skottman H,et al.Human cell-based micro electrode array platform for studying neurotoxicity.Front Neuroeng,2010,3:111.
[39]Cicero CE,Mostile G,Vasta R,et al.Metals and neurodegenerative diseases.A systematic review.Environ Res,2017,159:82-94.
[40]Caito S,Aschner M.Neurotoxicity of metals.Handb Clin Neurol,2015,131:169-189.
[41]Chandravanshi LP,Gupta R,Shukla RK.Developmental neurotoxicity of arsenic:involvement of oxidative stress and mitochondrial functions.Biol Trace Elem Res,2018,186(1):185-198.
[42]Cariccio VL,SamàA,Bramanti P,et al.Mercury involvement in neuronal damage and in neurodegenerative diseases.Biol Trace Elem Res,2019,187(2):341-356.
[43]Pamies D,Block K,Lau P,et al.Rotenone exerts developmental neurotoxicity in a human brain spheroid model.Toxicol Appl Pharmacol,2018,354:101-114.
[44]O'Carroll D,Schaefer A.General principals of miRNA biogenesis and regulation in the brain.Neuropsychopharmacology,2013,38(1):39-54.
[45]Jiang C,Logan S,Yan Y,et al.Signaling network between the dysregulated expression of microRNAs and m RNAs in propofol-induced developmental neurotoxicity in mice.Sci Rep,2018,8(1):14172.
[46]Schmuck MR,Temme T,Dach K,et al.Omnisphero:a high-content image analysis(HCA)approach for phenotypic developmental neurotoxicity(DNT)screenings of organoid neurosphere cultures in vitro.Arch Toxicol,2017,91(4):2017-2028.
[47]Joshi P,Yu K,Kang S,et al.3D-cultured neural stem cell microarrays on a micropillar chip for high-throughput developmental neurotoxicology.Exp Cell Res,2018,370(2):680-691.
[48]Gazina EV,Morrisroe E,Mendis GDC,et al.Method of derivation and differentiation of mouse embryonic stem cells generating synchronous neuronal networks.J Neurosci Methods,2018,293:53-58.
[49]Sandstr?m J,Eggermann E,Charvet I,et al.Development and characterization of a human embryonic stem cell-derived 3D neural tissue model for neurotoxicity testing.Toxicol In Vitro,2017,38:124-135.
[50]Seidel D,Obendorf J,Englich B,et al.Impedimetric real-time monitoring of neural pluripotent stem cell differentiation process on microelectrode arrays.Biosens Bioelectron,2016,86:277-286.
[51]Harry GJ,Billingsley M,Bruinink A,et al.In vitro techniques for the assessment of neurotoxicity.Environ Health Perspect,1998,106 Suppl 1:131-158.
[2]Evans MJ,Kaufman MH.Establishment in culture of pluripotential cells from mouse embryos.Nature,1981,292(5819):154-156.
[3]Spielmann H,Pohl I,Doring B,et al.The embryonic stem cell test(EST),an in vitro embryotoxicity test using two permanent mouse cell lines:3T3 fibroblasts and embryonic stem cells.In Vitro Toxicol,1997,10(1):119-127.
[4]Aikawa N,Kunisato A,Nagao K,et al.Detection of thalidomide embryotoxicity by in vitro embryotoxicity testing based on human iPScells.J Pharmaco Sci,2014,124(2):201-207.
[5]Fang H,Zhi Y,Yu Z,et al.The embryonic toxicity evaluation of deoxynivalenol(DON)by murine embryonic stem cell test and human embryonic stem cell test models.Food Control,2018,86:234-240.
[6]Luo S,Fang HQ,Yang H,et al.Comparison of embryotoxicity of di(2-ethylhexyl)phthalate using mouse and human embryonic stem cell test models in vitro.Chin J Prev Med,2016,50(7):645-651.(in Chinese)罗莎,方海琴,杨辉,等.两种胚胎干细胞实验模型评价邻苯二甲酸(2-乙基己基)酯胚胎发育毒性的比较研究.中华预防医学杂志,2016,50(7):645-651.
[7]Rice D,Barone SJ.Critical periods of vulnerability for the developing nervous system:evidence from humans and animal models.Environ Health Perspect,2000,108 Suppl 3:511-533.
[8]Kraft AD,Aschner M,Cory-Slechta DA,et al.Unmasking silent neurotoxicity following developmental exposure to environmental toxicants.Neurotoxicol Teratol,2016,55:38-44.
[9]Grandjean P,Landrigan PJ.Neurobehavioural effects of developmental toxicity.Lancet Neurol,2014,13(3):330-338.
[10]Yi H,Xie B,Liu B,et al.Derivation and identification of motor neurons from human urine-derived induced pluripotent stem cells.Stem Cells Int,2018,2018:3628578.
[11]Santos DP,Kiskinis E.Generation of spinal motor neurons from human pluripotent stem cells.Methods Mol Biol,2017,1538:53-66.
[12]Datta I,Ganapathy K,Tattikota SM,et al.Directed differentiation of human embryonic stem cell-line HUES9 to dopaminergic neurons in a serum-free defined culture niche.Cell Biol Int,2013,37(1):54-64.
[13]Kawasaki H,Mizuseki K,Nishikawa S,et al.Induction of midbrain dopaminergic neurons from ES cells by stromal cell-derived inducing activity.Neuron,2000,28(1):31-40.
[14]Hu Y,Qu ZY,Cao SY,et al.Directed differentiation of basal forebrain cholinergic neurons from human pluripotent stem cells.J Neurosci Methods,2016,266:42-49.
[15]Vazin T,Ball K A,Lu H,et al.Efficient derivation of cortical glutamatergic neurons from human pluripotent stem cells:a model system to study neurotoxicity in Alzheimer's disease.Neurobiol Dis,2014,62:62-72.
[16]Pei Y,Peng J,Behl M,et al.Comparative neurotoxicity screening in human iPSC-derived neural stem cells,neurons and astrocytes.Brain Res,2016,1638(Pt A):57-73.
[17]Bian J,Zheng J,Li S,et al.Sequential differentiation of embryonic stem cells into neural epithelial-like stem cells and oligodendrocyte progenitor cells.PLoS One,2016,11(5):e155227.
[18]Arber C,Precious SV,Cambray S,et al.Activin A directs striatal projection neuron differentiation of human pluripotent stem cells.Development,2015,142(7):1375-1386.
[19]Reichman S,Slembrouck A,Gagliardi G,et al.Generation of storable retinal organoids and retinal pigmented epithelium from adherent human iPS cells in Xeno-free and feeder-free conditions.Stem Cells,2017,35(5):1176-1188.
[20]Matsuoka AJ,Morrissey ZD,Zhang C,et al.Directed differentiation of human embryonic stem cells toward placode-derived spiral ganglion-like sensory neurons.Stem Cells Transl Med,2017,6(3):923-936.
[21]Pamies D,Barreras P,Block K,et al.A human brain microphysiological system derived from induced pluripotent stem cells to study neurological diseases and toxicity.ALTEX,2017,34(3):362-376.
[22]Schwartz MP,Hou Z,Propson NE,et al.Human pluripotent stem cell-derived neural constructs for predicting neural toxicity.Proc Natl Acad Sci U S A,2015,112(40):12516-12521.
[23]Druwe I,Freudenrich TM,Wallace K,et al.Sensitivity of neuroprogenitor cells to chemical-induced apoptosis using a multiplexed assay suitable for high-throughput screening.Toxicology,2015,333:14-24.
[24]Tiwari SK,Agarwal S,Chauhan LK,et al.Bisphenol-A impairs myelination potential during development in the hippocampus of the rat brain.Mol Neurobiol,2015,51(3):1395-1416.
[25]Visan A,Hayess K,Sittner D,et al.Neural differentiation of mouse embryonic stem cells as a tool to assess developmental neurotoxicity in vitro.Neurotoxicology,2012,33(5):1135-1146.
[26]Guan S,Xu J,Guo Y,et al.Pyrroloquinoline quinone against glutamate-induced neurotoxicity in cultured neural stem and progenitor cells.Int J Dev Neurosci,2015,42:37-45.
[27]Augustyniak J,Lenart J,Zychowicz M,et al.Sensitivity of hiPSC-derived neural stem cells(NSC)to Pyrroloquinoline quinone depends on their developmental stage.Toxicol In Vitro,2017,45(Pt 3):434-444.
[28]Menzner AK,Gilbert DF.A protocol for in vitro High-throughput chemical susceptibility screening in differentiating NT2 stem cells.Methods Mol Biol,2017,1601:61-70.
[29]Inohana M,Eguchi A,Nakamura M,et al.Developmental exposure to aluminum chloride irreversibly affects postnatal hippocampal neurogenesis involving multiple functions in mice.Toxicological Sci,2018,164(1):264-277.
[30]Yang D,Lein PJ.Polychlorinated biphenyls increase apoptosis in the developing rat brain.Curr Neurobiol,2010,1(1):70-76.
[31]Mori H,Sasaki G,Nishikawa M,et al.Effects of subcytotoxic cadmium on morphology of glial fibrillary acidic protein network in astrocytes derived from murine neural stem/progenitor cells.Environ Toxicol Pharmacol,2015,40(2):639-644.
[32]Parsons-White AB,Spitzer N.Environmentally relevant manganese overexposure alters neural cell morphology and differentiation in vitro.Toxicol In Vitro,2018,50:22-28.
[33]Zimmer B,Lee G,Balmer NV,et al.Evaluation of developmental toxicants and signaling pathways in a functional test based on the migration of human neural crest cells.Environ Health Perspect,2012,120(8):1116-1122.
[34]Nyffeler J,Karreman C,Leisner H,et al.Design of a high-throughput human neural crest cell migration assay to indicate potential developmental toxicants.ALTEX,2017,34(1):75-94.
[35]Ryan KR,Sirenko O,Parham F,et al.Neurite outgrowth in human induced pluripotent stem cell-derived neurons as a high-throughput screen for developmental neurotoxicity or neurotoxicity.Neurotoxicology,2016,53:271-281.
[36]Rai NK,Ashok A,Rai A,et al.Exposure to As,Cd and Pb-mixture impairs myelin and axon development in rat brain,optic nerve and retina.Toxicol Appl Pharmacol,2013,273(2):242-258.
[37]Barateiro A,Fernandes A.Temporal oligodendrocyte lineage progression:in vitro models of proliferation,differentiation and myelination.Biochim Biophys Acta,2014,1843(9):1917-1929.
[38]Yl?-Outinen L,Heikkil?J,Skottman H,et al.Human cell-based micro electrode array platform for studying neurotoxicity.Front Neuroeng,2010,3:111.
[39]Cicero CE,Mostile G,Vasta R,et al.Metals and neurodegenerative diseases.A systematic review.Environ Res,2017,159:82-94.
[40]Caito S,Aschner M.Neurotoxicity of metals.Handb Clin Neurol,2015,131:169-189.
[41]Chandravanshi LP,Gupta R,Shukla RK.Developmental neurotoxicity of arsenic:involvement of oxidative stress and mitochondrial functions.Biol Trace Elem Res,2018,186(1):185-198.
[42]Cariccio VL,SamàA,Bramanti P,et al.Mercury involvement in neuronal damage and in neurodegenerative diseases.Biol Trace Elem Res,2019,187(2):341-356.
[43]Pamies D,Block K,Lau P,et al.Rotenone exerts developmental neurotoxicity in a human brain spheroid model.Toxicol Appl Pharmacol,2018,354:101-114.
[44]O'Carroll D,Schaefer A.General principals of miRNA biogenesis and regulation in the brain.Neuropsychopharmacology,2013,38(1):39-54.
[45]Jiang C,Logan S,Yan Y,et al.Signaling network between the dysregulated expression of microRNAs and m RNAs in propofol-induced developmental neurotoxicity in mice.Sci Rep,2018,8(1):14172.
[46]Schmuck MR,Temme T,Dach K,et al.Omnisphero:a high-content image analysis(HCA)approach for phenotypic developmental neurotoxicity(DNT)screenings of organoid neurosphere cultures in vitro.Arch Toxicol,2017,91(4):2017-2028.
[47]Joshi P,Yu K,Kang S,et al.3D-cultured neural stem cell microarrays on a micropillar chip for high-throughput developmental neurotoxicology.Exp Cell Res,2018,370(2):680-691.
[48]Gazina EV,Morrisroe E,Mendis GDC,et al.Method of derivation and differentiation of mouse embryonic stem cells generating synchronous neuronal networks.J Neurosci Methods,2018,293:53-58.
[49]Sandstr?m J,Eggermann E,Charvet I,et al.Development and characterization of a human embryonic stem cell-derived 3D neural tissue model for neurotoxicity testing.Toxicol In Vitro,2017,38:124-135.
[50]Seidel D,Obendorf J,Englich B,et al.Impedimetric real-time monitoring of neural pluripotent stem cell differentiation process on microelectrode arrays.Biosens Bioelectron,2016,86:277-286.
[51]Harry GJ,Billingsley M,Bruinink A,et al.In vitro techniques for the assessment of neurotoxicity.Environ Health Perspect,1998,106 Suppl 1:131-158.