健脑通胶囊对AD大鼠单胺氧化酶(MAO)影响的实验研究
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摘要
目的:观察健脑通胶囊对AD大鼠单胺氧化酶(MAO)的影响,从免疫组化方面探讨其作用机制。方法:从84只SD大鼠中随机选出12只(雌雄各半)为正常对照组,剩余72只进行AD模型复制,造模成功后死亡3只,剩余69只随机分成5组:AD模型组(12只)、脑复康组(12只)、健脑通胶囊小剂量组(15只)、健脑通胶囊中剂量组(15只)、健脑通胶囊大剂量组(15只);健脑通胶囊大、中、小剂量组分别予健脑通胶囊0.94g.kg-1·d-1、0.47g.kg-1·d-1、0.235g.kg-1·d-1灌胃,西药组给予脑复康片混悬液灌胃,剂量为50mg/mL,正常对照组予以等量生理盐水灌胃,每天上午给药1次,连续30d。结果:健脑通胶囊各组与脑复康组脑组织中单胺氧化酶(MAO)含量均高于模型组(P<0.05),且健脑通胶囊大、中剂量组优于小剂量组,差异有统计学意义(P<0.05)。结论:健脑通胶囊能减少AD模型大鼠单胺氧化酶(MAO)含量,延缓老年痴呆的发展,从而有效保护脑功能。
Objective: To observe the effect of Jiannaotong capsules on monoamine oxidase(MAO) level in rats with Alzheimer's disease(AD) and to investigate its action mechanism from the aspect of immunohistochemistry.Methods: Twelve rats(female-to-male ratio = 1: 1) were randomly selected from 84 AD rats as normal control group,and the other 72 rats were treated to establish an AD model.Three rats died while the model was induced.Sixty-nine surviving rats were randomly divided into AD model group(n = 12),piracetam group(n = 12),low-dose Jiannaotong capsule group(n = 15),middle-dose Jiannaotong capsule group(n = 15),and high-dose Jiannaotong capsule group(n = 15).The high-,middle-,and low-dose Jiannaotong capsule groups received Jiannaotong capsules(0.94g / kg.d,0.47 g / kg.d,and 0.235 g / kg.d,respectively) by gavage,the piracetam group received suspension of piracetam tablets(50 mg / ml) by gavage,and the normal control group received an equal volume of saline by gavage.Medication was given once in the morning every day for 30 consecutive days.Results: The Jiannaotong capsule groups and piracetam group had significantly higher MAO levels in brain tissue than the model group(P < 0.05),and the high-and middle-dose Jiannaotong capsule groups were significantly superior to the low-dose Jiannaotong capsule group in this regard(P < 0.05).Conclusion: Jiannaotong capsules can decrease MAO level in brain tissue in AD rats,thus delaying the progression of AD and protecting brain function.
Objective: To observe the effect of Jiannaotong capsules on monoamine oxidase(MAO) level in rats with Alzheimer's disease(AD) and to investigate its action mechanism from the aspect of immunohistochemistry.Methods: Twelve rats(female-to-male ratio = 1: 1) were randomly selected from 84 AD rats as normal control group,and the other 72 rats were treated to establish an AD model.Three rats died while the model was induced.Sixty-nine surviving rats were randomly divided into AD model group(n = 12),piracetam group(n = 12),low-dose Jiannaotong capsule group(n = 15),middle-dose Jiannaotong capsule group(n = 15),and high-dose Jiannaotong capsule group(n = 15).The high-,middle-,and low-dose Jiannaotong capsule groups received Jiannaotong capsules(0.94g / kg.d,0.47 g / kg.d,and 0.235 g / kg.d,respectively) by gavage,the piracetam group received suspension of piracetam tablets(50 mg / ml) by gavage,and the normal control group received an equal volume of saline by gavage.Medication was given once in the morning every day for 30 consecutive days.Results: The Jiannaotong capsule groups and piracetam group had significantly higher MAO levels in brain tissue than the model group(P < 0.05),and the high-and middle-dose Jiannaotong capsule groups were significantly superior to the low-dose Jiannaotong capsule group in this regard(P < 0.05).Conclusion: Jiannaotong capsules can decrease MAO level in brain tissue in AD rats,thus delaying the progression of AD and protecting brain function.
引文
[1]李文彬,张炳烈,马向展,等.吡乙酰胺对三氯化铝中毒大白鼠回避反应受损的对抗作用[J].中国药理与毒理学杂志,1988(2):1320.
[2]张江华,杨丽萍.启智胶囊治疗轻度认知障碍60例[J].陕西中医,2009,30(2):193-194.
[3]周大兴,李昌煜,张文龙,等.石菖蒲的促进小鼠学习记忆和提高耐缺氧力的作用[J].现代应用药学,1993,10(4):4.
[4]韩怡凡,陈先瑜.中枢胆碱能递质系统与学习记忆关系的研究[J].生理科学进展,1983,14(3):216.
[2]张江华,杨丽萍.启智胶囊治疗轻度认知障碍60例[J].陕西中医,2009,30(2):193-194.
[3]周大兴,李昌煜,张文龙,等.石菖蒲的促进小鼠学习记忆和提高耐缺氧力的作用[J].现代应用药学,1993,10(4):4.
[4]韩怡凡,陈先瑜.中枢胆碱能递质系统与学习记忆关系的研究[J].生理科学进展,1983,14(3):216.