水分逆境下PEG诱导引发种子对糯玉米芽苗的生理调控
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摘要
应用种子引发技术在不同土壤水分逆境条件下对糯玉米的出苗和幼苗生理调节进行了研究。结果表明,采用PEG诱导引发种子技术可拓宽糯玉米种子对水分逆境的适应范围,显著提高水分逆境下种子的发芽率和种子活力指数,并增强幼苗的根系活力,根尖更为明显。种子引发可增强幼苗抗氧化系统中APX、CAT、POD和SOD活性,尤其是APX和CAT变化更为活跃;另外,种子引发可改善游离氨基酸、还原糖、脯氨酸、可溶性糖和可溶性蛋白含量等渗透调节物质,较未引发处理减少膜质过氧化。PEG引发种子技术是应对土壤水分逆境下糯玉米种子发芽受阻和出苗困难的有效措施,可提高种子对水分逆境的耐受能力。
In this paper, seed priming technology was applied to study the emergence and physiological regulation of waxy maize seedling under different soil water stress conditions. The results showed that the PEG-induced seeding technique could broaden the adaptability of waxy maize seeds to water stress,significantly increase the seed germination rate and seed vigor index under water stress, and enhance the root vigor of seedlings, especially the root tip was more obvious. Seed priming can enhance the activity of APX, CAT, POD and SOD in seedling antioxidant system, especially the change of APX and CAT was more active. In addition, seed priming can improve free amino acid, reducing sugar, proline, soluble sugar and soluble protein content and other osmotic regulatory substances, and reduce membrane peroxidation compaire with untreated seed priming. PEG-induced seed technology is an effective measure to copy with the germination hindrance and seedling emergence difficulty of waxy corn seeds under soil water stress, which can improve the tolerance of seeds to water stress.
In this paper, seed priming technology was applied to study the emergence and physiological regulation of waxy maize seedling under different soil water stress conditions. The results showed that the PEG-induced seeding technique could broaden the adaptability of waxy maize seeds to water stress,significantly increase the seed germination rate and seed vigor index under water stress, and enhance the root vigor of seedlings, especially the root tip was more obvious. Seed priming can enhance the activity of APX, CAT, POD and SOD in seedling antioxidant system, especially the change of APX and CAT was more active. In addition, seed priming can improve free amino acid, reducing sugar, proline, soluble sugar and soluble protein content and other osmotic regulatory substances, and reduce membrane peroxidation compaire with untreated seed priming. PEG-induced seed technology is an effective measure to copy with the germination hindrance and seedling emergence difficulty of waxy corn seeds under soil water stress, which can improve the tolerance of seeds to water stress.
引文
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[2]王鹏,祝丽香,陈香香,等.种子引发对盐胁迫条件下桔梗种子萌发及幼苗生长的影响[J].山东农业科学,2017,49(09):71-76.
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[4]宋子叶,郭尚敬,杨万立,等.不同引发剂对小麦种子发芽的影响[J].河北农业科学,2018,22(04):13-15,21.
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[11]詹振楠,王文娟.种子引发对盐胁迫枸杞种子萌发的影响[J].广东农业科学,2018,45(06):14-18.
[12]祁瑞林,张红瑞,彭涛,等.不同引发剂对贮藏夏枯草种子引发效果的影响[J].浙江农业科学,2018,59(02):283-286.
[13]Foyer C,Fletcher J.Plant antioxidants:colour me healthy[J].Biologist (London,England).2001,48(3):115-120.
[14]Zhang C,He P,Yu Z,et al.Effect of zinc sulphate and PEG priming on ageing seed germination and antioxidase activities of Perilla frutescens seedlings[J].China Journal of Chinese Materia Medica.2010,35(18):2372-2377.
[15]Parida AK,Dagaonkar VS,Phalak MS,et al.Alterations in photosynthetic pigments,protein and osmotic components in cotton genotypes subjected to short-term drought stress followed by recovery[J].Plant Biotechnology Reports.2007,1(1):37-48.
[16]牛晓雪,牟萌,李保华,等.FeSO4引发提高秦艽种子萌发的生理机制[J].中国生态农业学报,2018(12):1828-1835.
[17]Chen K,Arora R.Dynamics of the antioxidant system during seed osmopriming,post-priming germination,and seedling establishment in Spinach (Spinacia oleracea)[J].Plant Science.2011,180(2):212-220.
[2]王鹏,祝丽香,陈香香,等.种子引发对盐胁迫条件下桔梗种子萌发及幼苗生长的影响[J].山东农业科学,2017,49(09):71-76.
[3]古吉,马文广.不同萌发温度下红花大金元种子引发温度的筛选研究[J].种子,2017,36(03):16-20.
[4]宋子叶,郭尚敬,杨万立,等.不同引发剂对小麦种子发芽的影响[J].河北农业科学,2018,22(04):13-15,21.
[5]Hur S.Effect of osmoconditioning on the productivity of Italian ryegrass and sorghum under suboptimal conditions.Korean Journal of Animal Sciences (Korea Republic).1991.
[6]Yuan-Yuan S,Yong-Jian S,Ming-Tian W,et al.Effects of seed priming on germination and seedling growth under water stress in rice[J].Acta Agronomica Sinica.2010,36(11):1931-1940.
[7]孙璐,周宇飞,汪澈,等.高粱品种萌发期耐盐性筛选与鉴定[J].中国农业科学,2012,45(09):1714-1722.
[8]张宪政.作物生理研究法[M].北京:农业出版社,1992.
[9]Gao Y-P,Young L,Bonham-Smith P,et al.Characterization and expression of plasma and tonoplast membrane aquaporins in primed seed of Brassica napus during germination under stress conditions[J].Plant Molecular Biology.1999,40(4):635-644.
[10]Hur S.Effect of osmoconditioning on the productivity of Italian ryegrass and sorghum under suboptimal conditions[J].Korean Journal of Animal Sciences (Korea Republic).1991.
[11]詹振楠,王文娟.种子引发对盐胁迫枸杞种子萌发的影响[J].广东农业科学,2018,45(06):14-18.
[12]祁瑞林,张红瑞,彭涛,等.不同引发剂对贮藏夏枯草种子引发效果的影响[J].浙江农业科学,2018,59(02):283-286.
[13]Foyer C,Fletcher J.Plant antioxidants:colour me healthy[J].Biologist (London,England).2001,48(3):115-120.
[14]Zhang C,He P,Yu Z,et al.Effect of zinc sulphate and PEG priming on ageing seed germination and antioxidase activities of Perilla frutescens seedlings[J].China Journal of Chinese Materia Medica.2010,35(18):2372-2377.
[15]Parida AK,Dagaonkar VS,Phalak MS,et al.Alterations in photosynthetic pigments,protein and osmotic components in cotton genotypes subjected to short-term drought stress followed by recovery[J].Plant Biotechnology Reports.2007,1(1):37-48.
[16]牛晓雪,牟萌,李保华,等.FeSO4引发提高秦艽种子萌发的生理机制[J].中国生态农业学报,2018(12):1828-1835.
[17]Chen K,Arora R.Dynamics of the antioxidant system during seed osmopriming,post-priming germination,and seedling establishment in Spinach (Spinacia oleracea)[J].Plant Science.2011,180(2):212-220.