带鱼蛋白水解肽-金属配合物制备工艺
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摘要
为提高带鱼下脚料的利用率为目的,以带鱼下脚料为原料,用木瓜蛋白酶水解,并分别与氯化亚铁、氯化镁、氯化锌和氯化钙螯合,采用单因素试验及正交试验进行优化,测定最佳螯合条件。研究表明:当金属元素添加量为2%时,带鱼蛋白水解肽-金属配合物(铁肽、钙肽、镁肽和锌肽)最佳螯合工艺条件为:螯合时间30 min, pH 7.0,温度30℃。此时铁肽、钙肽、镁肽和锌肽最大螯合率分别为68.9%, 63.2%, 65.7%和42.3%。
To improve the utilization rate of the waste, the fish waste was hydrolysed by papain from hairtail, which was chelated with ferrous chloride, magnesium chloride, zinc chloride and calcium chloride separately. The optimum enzymatic hydrolysis conditions and chelation conditions were determined by signal-factor and orthogonal experiments. The research showed that the best chelation conditions was 30 ℃, pH 7.0, and 30 min. Under the best condition, the rates of chelation for PH-Fe~(2+), PH-Zn~(2+), PH-Ca~(2+) and PH-Mg~(2+) were 68.9%, 65.7%, 63.2% and 42.3%, respectively.
To improve the utilization rate of the waste, the fish waste was hydrolysed by papain from hairtail, which was chelated with ferrous chloride, magnesium chloride, zinc chloride and calcium chloride separately. The optimum enzymatic hydrolysis conditions and chelation conditions were determined by signal-factor and orthogonal experiments. The research showed that the best chelation conditions was 30 ℃, pH 7.0, and 30 min. Under the best condition, the rates of chelation for PH-Fe~(2+), PH-Zn~(2+), PH-Ca~(2+) and PH-Mg~(2+) were 68.9%, 65.7%, 63.2% and 42.3%, respectively.
引文
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[12]钟国清.氨基酸微量元素鳌合物的制备方法研究[J].饲料工业,2004,25(1):47-48.
[2]黄镇繁.带鱼下脚料蛋白质提取与分离研究[J].渔业现代化,2008,3(5):52-55.
[3]SARAH G,PAULE B N,JUSTIN L,et al.Ability of GHTD-amide and analogs to enhance insulin activity through zinc chelation and dispersal of insulin oligomers[J].Peptides,2009,30:1088-1097.
[4]AMBAR A,VANESSA S,EMMANUELLE M,et al.Zinc(Ⅱ)binds to the neuroprotective peptide humanin[J].Journal of Inorganic Biochemistry,2006,100:1672-1678.
[5]林慧敏.带鱼下脚料酶解小肽亚铁螯合物结构鉴定及其生物活性研究[D].福州:福建农林大学,2012.
[6]宁正详.食品成分分析手册[M].北京:中国轻工业出版社,1998.
[7]肖珊.乳清蛋白肽螯合钙的制备及其微胶囊化的研究[D].广州:华南理工大学,2014.
[8]赵立娜,汪少芸,张亚娇,等.响应面法优化乳清蛋白肽螯合钙离子的研究[J].中国食品学报,2014,14(4):64-71.
[9]罔伟.真鲷鱼鱗皮胶原肽锌的制取、结构和性质研究[D].天津:天津科技大学,2013.
[10]俞园园.类人胶原蛋白-锌螯合物的制备及相关研究[J].中国食物与营养,2013,19(8):46-49.
[11]崔潇,江虹锐,刘小玲,等.响应面法优化罗非鱼鱼皮胶原多肽螯合镁的工艺条件的研究[J].食品工业科技,2013,24(15):238-245.
[12]钟国清.氨基酸微量元素鳌合物的制备方法研究[J].饲料工业,2004,25(1):47-48.