摘要
目的建立改进的QuEChERS-超高效液相色谱-串联质谱法测定毛木耳中23种植物生长调节剂的分析方法。方法样品经1%乙酸乙腈溶液提取后,比较了C_(18)和PEP-2的净化和去除色素能力,同时对提取试剂和PEP-2净化剂用量进行了优化,采用超高效液相色谱-串联质谱测定。结果在优化的条件下,23种植物生长调节剂在相关范围内线性关系良好(r>0.999),方法定量限为0.1~5μg/kg,在0.01、0.05、0.1 mg/kg 3个添加浓度下平行6次实验,回收率为80.6%~116.6%,RSD为0.9%~11.2%。结论该法满足国内外植物生长调节剂残留限量要求,可为食用菌中植物生长调节剂残留检测提供参考。
Objective To establish a method for determination of 23 plant growth regulators in Auricularia cornea by modified QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry(UPLS-MS/MS). Methods The samples were extracted with 0.1% acetic acetonitrile solution, then the capacity of purification and pigment removal of C_(18)and PEP-2 were compared. At the same time, the amount of extraction reagent and PEP-2 purifier was optimized, and samples were detected by UPLC-MS/MS. Results Under the optimum conditions, 23 plant growth regulators had linear relationships(r>0.999), and the limits of quantitation were ranged from 0.1 to 5 μg/kg. The recoveries at 3 spiked levels(0.01, 0.05 and O.lmg/kg) were 82.6%-116.6%, with the relative standard deviations(RSD) of 0.9%-11.2%(n=6). Conclusion This method is satisfied the demand of the plant growth regulators residue limits, which can provide technical support for detection of plant growth regulator residues in edible fungi.
引文
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