电针不同腧穴配伍对心肌缺血损伤大鼠心肌功能的影响
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摘要
目的探讨电针不同腧穴配伍对心肌缺血损伤大鼠心肌功能的影响。方法按随机数字表法将60只雄性Wistar大鼠分为正常组、模型组、腧穴配伍组、常规配穴组四组,每组各15只。造模方法 :盐酸异丙肾上腺素皮下注射(5 m L/kg)。正常组注射同等剂量的0.9%氯化钠溶液,1次/d,连续1周;模型组造模;腧穴配伍组取造模成功的大鼠,电针其双内关穴、双足三里穴及关元穴,每天各1次,连续2周;常规配穴组取双内关、双神门及膻中,余法同腧穴配伍组。观察各组大鼠心电图变化及心肌超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量。结果与正常组比较,模型组心率(HR)加快明显,S-T段振幅抬高显著,差异有高度统计学意义(P<0.01);电针干预后,腧穴配伍组及常规配穴组HR有所减慢,S-T段振幅抬高降低,与模型组比较,差异有统计学意义(P<0.05)。与正常组比较,模型组大鼠心肌SOD活性、GSH-Px活性显著降低,MDA含量显著升高,差异有高度统计学意义(P<0.01);电针干预后,腧穴配伍组及常规配穴组大鼠心肌SOD活性、GSHPx活性显著升高,MDA含量降低,与模型组比较,差异有统计学意义(P<0.05);与常规配穴组比较,腧穴配伍组心肌SOD、GSH-Px活性升高,MDA含量降低,差异有统计学意义(P<0.05)。结论腧穴配伍针刺法具有很好的保护心肌缺血的效果,能够有效减轻心肌细胞变性及心肌组织的损害,为临床运用针灸治疗心肌缺血性疾病提供依据。
Objective To explore the effect of different acupoints of electroacupuncture on myocardial function in rats with myocardial ischemia injury. Methods Sixty male Wistar rats were randomly divided into four groups by random number table method: normal group, model group, acupoint compatibility group, normal distribution group, with 15 rats in each group. Building methods: subcutaneous injection of Isoproterenol Hydrochloride(5 mL/kg) was given. Normal group: the same dose of 0.9% sodium chloride solution was injected, once a day, for one week. Model group was made the model. Acupoint compatibility group: the successful rats of the model were taken, and each day, the electric needle of the double "neiguan", double "zusanli" and "guanyuan" were performed respectively for two weeks. Normal distribution group: "neiguan", double "shenmen" and "danzhong" were pe rformed respectively, the followed method was as the same as the acupoint compatibility group. The changes of electrocardiogram in rats, and the activities of superoxide dismutase(SOD), glutathione peroxidase(GSH-Px) and malondialdehyde(MDA) were observed. Results Compared with normal group, the heart rate(HR) in model group was accelerated significantly, and the amplitude of S-T segment was significantly enhanced, the differences were highly statistically significant(P < 0.01). After the electroacupuncture intervention, the HR of the acupoints in the acupoint compatibility group and normal distribution group was decreased,and the amplitude of S-T was decreased, which had statistically significant differences compared with those of model group(P < 0.05). Compared with the normalgroup, the activities of myocardial SOD and GSH-Px in the model group were decreased significantly, and the content of MDA was increased significantly, the differences were highly statistically significant(P < 0.01). After the electroacupuncture intervention, the activities of SOD and GSH-Px of the myocardium of the rats in the acupoint compatibility group and normal distribution group were significantly increased, and the content of MDA was decreased, which had statistically significant differences compared with those of model group(P < 0.05). Compared with normal distribution group, the activities of myocardial SOD and GSH-Px in the acupoint compatibility group were significantly increased, and the content of MDA was decreased significantly, the differences were statistically significant(P < 0.05).Conclusion Compatibility of acupuncture acupoints method has very good protection effect for myocardial ischemia,which can effectively reduce the myocardial cell degeneration and the myocardial tissue damage, and it can provide basis for clinical controlling the myocardial ischemic diseases with acupuncture.
Objective To explore the effect of different acupoints of electroacupuncture on myocardial function in rats with myocardial ischemia injury. Methods Sixty male Wistar rats were randomly divided into four groups by random number table method: normal group, model group, acupoint compatibility group, normal distribution group, with 15 rats in each group. Building methods: subcutaneous injection of Isoproterenol Hydrochloride(5 mL/kg) was given. Normal group: the same dose of 0.9% sodium chloride solution was injected, once a day, for one week. Model group was made the model. Acupoint compatibility group: the successful rats of the model were taken, and each day, the electric needle of the double "neiguan", double "zusanli" and "guanyuan" were performed respectively for two weeks. Normal distribution group: "neiguan", double "shenmen" and "danzhong" were pe rformed respectively, the followed method was as the same as the acupoint compatibility group. The changes of electrocardiogram in rats, and the activities of superoxide dismutase(SOD), glutathione peroxidase(GSH-Px) and malondialdehyde(MDA) were observed. Results Compared with normal group, the heart rate(HR) in model group was accelerated significantly, and the amplitude of S-T segment was significantly enhanced, the differences were highly statistically significant(P < 0.01). After the electroacupuncture intervention, the HR of the acupoints in the acupoint compatibility group and normal distribution group was decreased,and the amplitude of S-T was decreased, which had statistically significant differences compared with those of model group(P < 0.05). Compared with the normalgroup, the activities of myocardial SOD and GSH-Px in the model group were decreased significantly, and the content of MDA was increased significantly, the differences were highly statistically significant(P < 0.01). After the electroacupuncture intervention, the activities of SOD and GSH-Px of the myocardium of the rats in the acupoint compatibility group and normal distribution group were significantly increased, and the content of MDA was decreased, which had statistically significant differences compared with those of model group(P < 0.05). Compared with normal distribution group, the activities of myocardial SOD and GSH-Px in the acupoint compatibility group were significantly increased, and the content of MDA was decreased significantly, the differences were statistically significant(P < 0.05).Conclusion Compatibility of acupuncture acupoints method has very good protection effect for myocardial ischemia,which can effectively reduce the myocardial cell degeneration and the myocardial tissue damage, and it can provide basis for clinical controlling the myocardial ischemic diseases with acupuncture.
引文
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[11]唐丹丽,刘寨华,隋宇,等.中药防治心肌缺血再灌注损伤炎症反应作用机制研究概况[J].中医杂志,2014,55(11):980-983.
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[13]路雅雯,嵇波,戴健,等.不同频率电针及刺激次数对心肌缺血大鼠内关穴与非经非穴点皮肤血流灌注量的影响[J].北京中医药大学学报,2016,39(12):1041-1047.
[14]王华,望庐山,梁凤霞,等.电针治疗对慢性心肌缺血大鼠心电图、心肌病理形态及PI3K/Akt信号通路的影响[J].中国针灸,2016,36(4):389-395.
[15]王华,卢继东,吴松,等.电针不同腧穴对心肌缺血模型大鼠细胞凋亡及心肌细胞mi RNAs表达的影响[J].中国针灸,2016,36(3):281-286.
[16]卢志强,张艳军,崔广智,等.心肌缺血模型的制作方法研究进展[J].中国药理学通报,2012,28(8):1053-1057.
[17]彭峰林,黄丽丽,郭艳菊,等.运动性心肌缺血损伤模型大鼠的制备[J].中国组织工程研究,2016,20(40):6007-6013.
[18]项红兵,梅伟,田玉科,等.心肌保护新亮点:中枢远端干预减轻心肌缺血再灌注损伤[J].中华麻醉学杂志,2016,36(7):769-770.
[19]Zhou YH,Han QF,Wang LH,et al.High mobility group box 1 protein attenuates myocardial ischemia reperfusion injury via inhibition of the p38 mitogen-activated protein kinase signalingpathway[J].Exp Ther Med,2017,14(2):1582-1588.
[20]孙彦辉,梁玉磊,张闯,等.不同时间电针对血管性痴呆小鼠学习记忆能力与海马氧自由基的影响[J].中国老年学杂志,2016,36(7):1549-1551.
[21]Liu X,Yu Z,Huang X,et al.Peroxisome proliferator-activated receptorγ(PPARγ)mediates the protective effect of quercetin against myocardial ischemia-reperfusion injury via suppressing the NF-κB pathway[J].Am J Transl Res,2016,8(12):5169-5186.
[2]Reczuch K,Milewski K,W諭sek W,et al.Bioresorbable scaffolds in the treatment ofcoronary artery disease.Expert consensus statement of the Association of Cardiovascular Interventions of the Polish Cardiac Society(ACVI PCS)[J].Kardiol Pol,2017,75(8):817-835.
[3]Michniewicz E,Mlodawska E,Lopatowska P,et al.Patients with atrial fibrillation and coronary artery disease-Double trouble[J].Adv Med Sci,2017,63(1):30-35.
[4]陈茜.标本配穴电针干预对慢性心肌缺血模型大鼠心肌线粒体结构功能影响研究[D].武汉:湖北中医药大学,2016.
[5]谢俊,吴松,梁凤霞,等.不同配穴预处理对心肌缺血模型大鼠抗自由基酶的影响[J].湖北中医药大学学报,2014,16(6):5-7.
[6]成泽东,俞耸在,陈以国.循经针刺对心肌缺血大鼠心肌组织Toll样受体4蛋白的调控作用[J].北京中医药大学学报,2016,39(2):173-176.
[7]李梦,胡玲,蔡荣林,等.电针内关、心俞穴对急性心肌缺血大鼠脊髓背根神经电活动及下丘脑室旁核去甲肾上腺素、多巴胺浓度的影响[J].中西医结合学报,2012,10(8):874-879.
[8]谢俊.“标本配穴”法电针防治大鼠心肌缺血损伤及其线粒体信号通路调控机制[D].武汉:湖北中医药大学,2016.
[9]胡元亮.小动物针灸技法手册[M].北京:化学工业出版社,2009:131-134.
[10]华兴邦.大鼠穴位图谱的研制[J].实验动物与动物实验,1991,11(6):1-5.
[11]唐丹丽,刘寨华,隋宇,等.中药防治心肌缺血再灌注损伤炎症反应作用机制研究概况[J].中医杂志,2014,55(11):980-983.
[12]袁秋贞,郑旭,刘富,等.心肌片对缺血再灌注心肌细胞凋亡的影响[J].陕西中医,2017,38(1):127-129.
[13]路雅雯,嵇波,戴健,等.不同频率电针及刺激次数对心肌缺血大鼠内关穴与非经非穴点皮肤血流灌注量的影响[J].北京中医药大学学报,2016,39(12):1041-1047.
[14]王华,望庐山,梁凤霞,等.电针治疗对慢性心肌缺血大鼠心电图、心肌病理形态及PI3K/Akt信号通路的影响[J].中国针灸,2016,36(4):389-395.
[15]王华,卢继东,吴松,等.电针不同腧穴对心肌缺血模型大鼠细胞凋亡及心肌细胞mi RNAs表达的影响[J].中国针灸,2016,36(3):281-286.
[16]卢志强,张艳军,崔广智,等.心肌缺血模型的制作方法研究进展[J].中国药理学通报,2012,28(8):1053-1057.
[17]彭峰林,黄丽丽,郭艳菊,等.运动性心肌缺血损伤模型大鼠的制备[J].中国组织工程研究,2016,20(40):6007-6013.
[18]项红兵,梅伟,田玉科,等.心肌保护新亮点:中枢远端干预减轻心肌缺血再灌注损伤[J].中华麻醉学杂志,2016,36(7):769-770.
[19]Zhou YH,Han QF,Wang LH,et al.High mobility group box 1 protein attenuates myocardial ischemia reperfusion injury via inhibition of the p38 mitogen-activated protein kinase signalingpathway[J].Exp Ther Med,2017,14(2):1582-1588.
[20]孙彦辉,梁玉磊,张闯,等.不同时间电针对血管性痴呆小鼠学习记忆能力与海马氧自由基的影响[J].中国老年学杂志,2016,36(7):1549-1551.
[21]Liu X,Yu Z,Huang X,et al.Peroxisome proliferator-activated receptorγ(PPARγ)mediates the protective effect of quercetin against myocardial ischemia-reperfusion injury via suppressing the NF-κB pathway[J].Am J Transl Res,2016,8(12):5169-5186.