芍药汤对致病性大肠杆菌的抑菌效果研究
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摘要
为观察芍药汤抗大肠杆菌的作用效果,试验分为体外牛津杯抑菌试验和小鼠体内抗大肠杆菌感染试验两部分。通过观察统计体外牛津杯抑菌试验中抑菌圈大小,小鼠的死亡数及死亡率,解剖小鼠观察其各个脏器的变化,血常规检测小鼠血液中白细胞、红细胞及血小板的变化。结果表明:芍药汤培养皿未出现抑菌圈。在预防及治疗试验中,芍药汤组小鼠死亡率及血液中各项白细胞指标均极显著低于阴性对照组(P<0.01);各项红细胞及血小板指标均极显著地高于阴性对照组(P<0.01)。通过解剖芍药汤组与阴性对照组小鼠,发现阴性对照组中小鼠小肠的肠系膜充血现象较为明显。说明芍药汤对感染大肠杆菌的小鼠具有较好的防治效果,但不是通过直接抑制大肠杆菌实现的。
In order to observe the effect of Shaoyao Decoction against Escherichia coli, the experiment was divided into two parts: the in vitro Oxford Cup antibacterial test and the mouse anti-E. coli infection test. By observing the size of the inhibition zone in the Oxford Cup antibacterial test in vitro, the death and mortality of the mice, the mice were dissected to observe the changes of various organs, the changes of white blood cells, red blood cells and platelets in the blood of mice were routinely detected by blood. The results showed that there was no inhibition zone in the Peony Petri dish. In the prevention and treatment trials, the mortality and blood leukocyte indexes in the Shaoyao Decoction group were significantly lower than those in the negative control group(P<0.01), the red blood cell and platelet indexes were significantly higher than the negative control group(P<0.01). By dissecting the Shaoyao Decoction group and the negative control group, it was found that the mesenteric congestion of the small intestine of the negative control group was more obvious. It is indicated that Shaoyaotang has good control effect on mice infected with E. coli, but it is not achieved by directly inhibiting E. coli.
In order to observe the effect of Shaoyao Decoction against Escherichia coli, the experiment was divided into two parts: the in vitro Oxford Cup antibacterial test and the mouse anti-E. coli infection test. By observing the size of the inhibition zone in the Oxford Cup antibacterial test in vitro, the death and mortality of the mice, the mice were dissected to observe the changes of various organs, the changes of white blood cells, red blood cells and platelets in the blood of mice were routinely detected by blood. The results showed that there was no inhibition zone in the Peony Petri dish. In the prevention and treatment trials, the mortality and blood leukocyte indexes in the Shaoyao Decoction group were significantly lower than those in the negative control group(P<0.01), the red blood cell and platelet indexes were significantly higher than the negative control group(P<0.01). By dissecting the Shaoyao Decoction group and the negative control group, it was found that the mesenteric congestion of the small intestine of the negative control group was more obvious. It is indicated that Shaoyaotang has good control effect on mice infected with E. coli, but it is not achieved by directly inhibiting E. coli.
引文
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[2]Xiangfeng Kong,Yuanliang Hu,Rong Rui,et al.Effects of Chinese herbal medicinal in‐gredients on peripherallym-phoeyte prolifera‐tion and serum antibody titer after vacc-ina‐tion in chiekens[J].International Immuno‐pharmacology,2004(7):975-982.
[3]陈希,穆祥,许剑琴.抗菌中药筛选思路的解析及实践[J].中国兽药杂志,2016,50(2):1-6.
[4]罗敏,李帅军,胡响当,等.芍药汤对胃肠湿热型溃疡性结肠炎大鼠结肠黏膜核因子-κB DNA结合活性的影响[J].中国医药导报,2015,12(3):4-6,169.
[5]张保国,梁晓夏,刘庆芳.黄芩汤现代药效学研究与临床运用[J].中国中药杂志,2008(22):2587-2590.
[6]张苏,王志斌,王跃,等.小檗碱在溃疡性结肠炎中作用及机制的研究进展[J].国际消化病杂志,2016,36(3):136-138,193.
[7]LI YH,XIAO HT,HU DD,et al.Berberine ame‐liorateschronic relapsing dextran sulfate so‐dium-induced colitisin C57BL/6 mice by sup‐pressing Th17 responses[J].Pharmacol Res,2016,110:227-239.
[8]ZHANG LC,WANG Y,TONG LC,et al.Berberine alleviatesdextran sodium sulfate-induced colitis by improvingintestinal barrier func‐tion and reducing inflammation andoxidative stress[J].Exp Ther Med,2017,13(6):3374-3382.
[9]潘琦虹,李燕珍,刘端勇,等.当归多糖对结肠炎小鼠peyer's patches结T淋巴细胞亚群水平的调控作用[J].江西中医药,2015,46(10):37-38,46.
[10]郭振军.大黄多糖治疗溃疡性结肠炎的机制研究[D].广州:第四军医大学,2013.
[11]刘海荣,马露,唐方,等.木香对溃疡性结肠炎大鼠干预作用的影响[J].世界华人消化杂志,2016,24(33):4467-4473.
[12]岳云升,李仲兴,王秀华,等.用新方法进行大黄对308株临床菌株抗菌活性研究[J].航空航天医药,2004(1):16-18.
[13]刘荣欣,鲁改儒,郭吉勇.中药及其组方对大肠杆菌的体外抑菌试验[J].安徽农业科学,2011,39(4):2265-2267.
[14]邱妍,王志蕊,李雯静,等.几种常见中药对大肠杆菌的体内外抑菌效果[J].畜牧兽医科技信息,2012(10):29-31.
[15]任志华,王婵,邓俊良,等.中药组方对腹腔注射大肠杆菌小鼠血清Th17、Treg型细胞因子的影响[J].中国兽医学报,2017,37(12):2378-2383.
[16]杨建英,黄名钱,肖莉春,等.中药复方对大肠杆菌攻毒小鼠的保护作用[J].天然产物研究与开发,2015,27(9):1607-1611.
[17]邸秀梅,肖秀玲,严绥平.黄芩药理学作用研究进展[J].内蒙古中医药,2007(4):57-59.