健脾灸对佐剂性关节炎大鼠心肌超微结构、RLX、ET及NO的影响
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摘要
目的:观察健脾灸对佐剂性关节炎(adjuvant arthritis,AA)大鼠心肌超微结构的影响及AA大鼠松弛素(relaxin,RLX)和内皮素-1(endothelin,ET-1)及一氧化氮(nitric oxide,NO)水平的影响。方法:将52只Wistar雄性大鼠随机分为空白组、模型组、药物组和艾灸组,每组13只。各组大鼠右后足内注射弗氏完全佐剂(Freund′s complete adjuvant,CFA)致炎,复制成AA模型。分别在造模前1 d、致炎后每3天、治疗第20天检测4组大鼠体质量、足跖肿胀度及关节炎指数(arthritis index,AI)。治疗20 d后,采用电镜观察大鼠心肌超微结构的变化,同时采用实时荧光定量PCR、Western blot法检测AA模型大鼠RLX和ET-NO系统各指标改变情况。结果:与空白组比较,其他3组大鼠心肌超微结构均有损伤,其中模型组较为明显。与模型组比较,艾灸组、药物组大鼠治疗后RLX、NO水平显著降低,ET-1显著升高(P<0.05或P<0.01);足跖肿胀度、关节炎指数显著降低(P<0.01)。结论:健脾灸可以改善AA大鼠心肌超微结构的损伤,降低关节炎指数、足跖肿胀度,降低RLX、NO水平,升高ET-1水平。
Objective:To observe the effects of Jianpi Moxibustion on myocardial ultrastructure,relaxin(RLX),endothelin(ET) and nitric oxide(NO) levels in rats with adjuvant arthritis(AA).Methods:52 Wistar male rats were randomly divided into blank group,model control group,drug treatment group and moxibustion treatment group,with 13 rats in each group.Rats in each group were injected with Freund′s complete adjuvant(CFA) into right hind foot to induce inflammation,and then duplicated into AA model.Body mass,foot swelling and arthritis index(AI) were measured 1 day before modeling,3 days after inflammation and 20 days after treatment.After 20 days of treatment,the changes of myocardial ultrastructure in rats were observed by electron microscopy,and the changes of RLX and ET-NO system in AA model rats were detected by real-time fluorescence quantitative PCR and Western blot.Results:Compared with the blank group,all the other three groups had myocardial ultrastructure damage,especially in the model group.Compared with the model group,the levels of RLX and NO in the moxibustion group and drug group were significantly lower.ET-1 was significantly higher(P<0.05 or P<0.01),and the swelling degree of the plantar and arthritis index were significantly lower(P<0.01).Conclusion:Jianpi Moxibustion can improve myocardial ultrastructure damage,reduce arthritis index,foot swelling degree,reduce RLX and NO levels,and increase ET-1 level in AA rats.
Objective:To observe the effects of Jianpi Moxibustion on myocardial ultrastructure,relaxin(RLX),endothelin(ET) and nitric oxide(NO) levels in rats with adjuvant arthritis(AA).Methods:52 Wistar male rats were randomly divided into blank group,model control group,drug treatment group and moxibustion treatment group,with 13 rats in each group.Rats in each group were injected with Freund′s complete adjuvant(CFA) into right hind foot to induce inflammation,and then duplicated into AA model.Body mass,foot swelling and arthritis index(AI) were measured 1 day before modeling,3 days after inflammation and 20 days after treatment.After 20 days of treatment,the changes of myocardial ultrastructure in rats were observed by electron microscopy,and the changes of RLX and ET-NO system in AA model rats were detected by real-time fluorescence quantitative PCR and Western blot.Results:Compared with the blank group,all the other three groups had myocardial ultrastructure damage,especially in the model group.Compared with the model group,the levels of RLX and NO in the moxibustion group and drug group were significantly lower.ET-1 was significantly higher(P<0.05 or P<0.01),and the swelling degree of the plantar and arthritis index were significantly lower(P<0.01).Conclusion:Jianpi Moxibustion can improve myocardial ultrastructure damage,reduce arthritis index,foot swelling degree,reduce RLX and NO levels,and increase ET-1 level in AA rats.
引文
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[13]舒晴,孙丹红,王华,等.针刺与艾灸对气虚证心率变异性的不同影响:随机对照研究[J].中国针灸,2017,37(1):25-30.
[14]高梓珊,甘君学,郭江燕,等.艾灸调控IL-17对实验性RA大鼠血清RANKL和TGF-β含量的影响[J].南京中医药大学学报,2016,32(6):543-547.
[15]殷媛,陈天帷,张瑞,等.艾灸对胶原诱导性关节炎大鼠血清白介素-17和肿瘤坏死因子α含量的影响[J].针刺研究,2017,42(2):149-152,177.
[16]丰小燕.浅谈痹症的病因及治疗[J].世界最新医学信息文摘,2015,15(19):98.
[2]李永梅,任立群.心肌间质细胞在心肌纤维化中的作用[J].心血管病学进展,2007,28(4):600-602.
[3]李忠仁.实验针灸学[M].2版.北京:中国中医药出版社,2007.
[4]郑利岩,张小卿,王熙,等.实验大鼠常用经穴检测与定位[J].辽宁中医杂志,2010,37(10):2058-2060.
[5]杨琴,杨桂枝,王蕾.雷公藤多苷对佐剂性关节炎模型大鼠踝关节相关指标的影响[J].中国临床康复,2017,47(10):189-192.
[6]熊英,顾一煌.艾灸预处理对急性力竭运动大鼠心肌损伤及蛋白激酶C的影响[J].中国老年学杂志,2016,36(7):1575-1577.
[7]徐璐.隔姜艾灸预防蒽环类药物所致心肌损害的实验研究[J].中国现代医生,2016,54(28):139-141.
[8]王茎,曾永蕾.艾灸对慢性心衰模型大鼠心功能及ET的影响[J].中医药临床杂志,2012,24(4):326-328.
[9]周杰华,郭雪申.宁心通脉包合物对冠心病患者血浆内皮素与一氧化氮水平的影响[J].中医临床研究,2015,1(3):6.
[10]CASTRO M G,RODRIGNEZ-PASCUAL F,MAGAN-MARCHAL N,et al.Screening of the endothelinl gene(EDNI) in a cohort of patients with essential left ventricuLar hypertrophy[J].Ann Hum Genet,2007,71(5):601-610.
[11]武凤琴,陈庆伟,王茎.艾灸调节机体免疫功能的研究进展[J].中医药临床杂志,2016,28(4):454-456.
[12]詹振吉,王健.艾灸对机体免疫系统的影响[J].中医学报,2016,31(3):449-452.
[13]舒晴,孙丹红,王华,等.针刺与艾灸对气虚证心率变异性的不同影响:随机对照研究[J].中国针灸,2017,37(1):25-30.
[14]高梓珊,甘君学,郭江燕,等.艾灸调控IL-17对实验性RA大鼠血清RANKL和TGF-β含量的影响[J].南京中医药大学学报,2016,32(6):543-547.
[15]殷媛,陈天帷,张瑞,等.艾灸对胶原诱导性关节炎大鼠血清白介素-17和肿瘤坏死因子α含量的影响[J].针刺研究,2017,42(2):149-152,177.
[16]丰小燕.浅谈痹症的病因及治疗[J].世界最新医学信息文摘,2015,15(19):98.