猪PRLR和FSHβ基因多态性检测及其与繁殖性状的关联分析
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摘要
试验旨在研究催乳素受体(prolactin receptor,PRLR)和卵泡刺激素β(follicle-stimulating hormoneβ,FSHβ)基因的多态性及其与母猪繁殖性状的关联分析。采用PCR-RFLP方法对大白猪、杜洛克猪和长白猪3个猪种共487头母猪进行了PRLR和FSHβ基因多态性检验,并采用单因子方差分析、LSD法分析不同基因型与母猪总产仔数、产活仔数、断奶窝重和断奶窝仔数等繁殖性状的相关性。结果表明,PRLR基因在大白猪和长白猪中B等位基因均为优势基因,基因频率分别为0.717和0.548,大白猪BB基因型总产仔数显著高于AA基因型(P<0.05),长白猪BB基因型断奶窝重显著高于AB基因型(P<0.05);FSHβ基因在大白猪、杜洛克猪和长白猪3个猪种中B等位基因均为优势基因,基因频率分别为0.804、0.760和0.789,长白猪BB基因型总产仔数和产活仔数均显著高于AB基因型(P<0.05),呈现BB>AA>AB趋势。因此,PRLR和FSHβ基因对荣昌猪场母猪繁殖性能有一定影响,可作为母猪繁殖性能分子选育的候选参考基因。
This study was aimed to investigate the polymorphism of prolactin receptor(PRLR) and follicle-stimulating hormone β(FSHβ) genes and its association with the reproductive traits in sows.The polymorphism of PRLR and FSHβ genes in 487 sows of Large White,Duroc and Landrace pigs was detected by PCR-RFLP method,and the association between different genotypes and reproductive traits such as total litter size,litter size,weaning litter weight and weaning litter size were analyzed by One-Way ANOVA analysis and LSD method.The results showed that B allele of PRLR gene was predominant allele in Large White and Landrace pigs,and the gene frequency were 0.717 and 0.548,respectively.The total number born of BB genotype in Large White pig was significantly higher than that of AA genotype(P<0.05),the weaning litter weight of BB genotype in Landrace pig was significantly higher than that of AB genotype(P<0.05).B allele of FSHβ gene had a high frequency in Large White,Duroc and Landrace pigs,and the gene frequency were 0.804,0.760 and 0.789,respectively.The total number born and number born alive of BB genotype in Large White pig was significantly higher than that of AB genotype(P<0.05),displaying BB>AA>AB trend.In conclusion,the polymorphism of PRLR and FSHβ genes significantly affected the reproductive traits of sows in Rongchang pigs farm,and they could be considered as candidate selective markers for molecular breeding of sows.
This study was aimed to investigate the polymorphism of prolactin receptor(PRLR) and follicle-stimulating hormone β(FSHβ) genes and its association with the reproductive traits in sows.The polymorphism of PRLR and FSHβ genes in 487 sows of Large White,Duroc and Landrace pigs was detected by PCR-RFLP method,and the association between different genotypes and reproductive traits such as total litter size,litter size,weaning litter weight and weaning litter size were analyzed by One-Way ANOVA analysis and LSD method.The results showed that B allele of PRLR gene was predominant allele in Large White and Landrace pigs,and the gene frequency were 0.717 and 0.548,respectively.The total number born of BB genotype in Large White pig was significantly higher than that of AA genotype(P<0.05),the weaning litter weight of BB genotype in Landrace pig was significantly higher than that of AB genotype(P<0.05).B allele of FSHβ gene had a high frequency in Large White,Duroc and Landrace pigs,and the gene frequency were 0.804,0.760 and 0.789,respectively.The total number born and number born alive of BB genotype in Large White pig was significantly higher than that of AB genotype(P<0.05),displaying BB>AA>AB trend.In conclusion,the polymorphism of PRLR and FSHβ genes significantly affected the reproductive traits of sows in Rongchang pigs farm,and they could be considered as candidate selective markers for molecular breeding of sows.
引文
[1] 曹果清.猪PRLR基因和FSHβ亚基基因多态性研究[D].太谷:山西农业大学,2004. CAO G Q.Studies on polymorphism of prolactin receptor gene and foolicle-stimulating hormone beta subunit gene in swine[D].Taigu:Shanxi Agricultural University,2004.(in Chinese)
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[3] SCHENNINK A,TROFF J F,FREKING B A,et al.A novel first exon directs hormone-sensitive transcription of the pig prolactin receptor[J].Journal of Molecular Endocrinol,2013,51(1):1-13.
[4] 林长光,朱志明,李盛霖,等.猪PRLR基因Alu Ⅰ多态与繁殖性状相关性的研究[J].福建农业学报,2008,23(2):137-140. LIN C G,ZHU Z M,LI S L,et al.Relationship between Alu Ⅰ polymorphism of PRLR gene and reproduction trait of swine[J].Fujian Journal of Agricultural Sciences,2008,23(2):137-140.(in Chinese)
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[6] JENKINS Z A,HENRY H M,SIZE J A,et al.Follistatin (FST) growth hormone receptor (GHR) and prolactin receptor (PRLR) genes map to the same region of sheep chromosome 16[J].Animal Genetics,2000,31(4):28-31.
[7] BALATSKVI V N,SAIENKO A M,PENA R N,et al.The genetic diversity of pig breeding on ten production quantitative traits loci[J].Cytology and Genetics,2015,49(5):299-307.
[8] DROGEMULLER C,HAMANN H,DISTL O.Candidate gene markers for litter size in different German pig lines[J].Journal of Animal Science,2001,79(10):2565-2570.
[9] VINCENT A L,EVANS G,SHORT T H.The prolactin receptor gene is associated with increased litter size in pig[J].Swine Research Report,1998,27:15-18.
[10] ROTHSCHILD M F,VINCENT A L,TUGGLE C K,et al.A mutation in the prolactin receptor gene is associated with increased litter size in pigs[J].Animal Genetic,1998,29(1):69.
[11] LINVILLE R C,POMP D,JOHNSON R K,et al.Candidate gene analysis for loci affecting litter size and ovulation rate in swine[J].Animal Science,2001,79(1):60-67.
[12] LI P F,MENG J Z,JING J J,et al.Study on the relationship between expression patterns of cocaine-and amphetamine regulated transcript and hormones secretion in porcine ovarian follicles[J].Biological Research,2018,51(1):6.
[13] IVA A,GIOVANNI L,FRANCESCA M,et al.An in vitro prototype of a porcine biomimetic testis-like cell culture system:A novel tool for the study of reassembled Sertoli and Leydig cells[J].Asian Journal of Andrology,2017,20(2):160-165.
[14] 赵要风,李宁,陈永福,等.猪FSHβ亚基基因5′端调控区的PCR克隆测序及变异分析[J].自然科学进展(国家重点实验室通迅),1996,6(3):351-356. ZHAO Y F,LI N,CHEN Y F,et al.PCR cloning sequencing and mutation analysis of 5′-terminal regulatory region of pig FSHβ subunit gene[J].Natural Science Advances (National Key Laboratory Communications),1996,6(3):351-356.(in Chinese)
[15] 陈克飞,黄路生,李宁,等.猪FSHβ及ESR合并基因型对产仔数性状的影响[J].科学通报,2000,45(18):1963-1966. CHEN K F,HUANG L S,LI N,et al.Effect of pig FSHβ mode and ESR combinational genotype on the number of offspring produced[J].Scientific Report,2000,45(18):1963-1966.(in Chinese)
[16] MELLINK C,LANBIBM Y,YERLE M,et al.PCR amplification and physical localization of the genes for pig FSHβ and LHB[J].Cytogenetics and Cell Genetics,1995,70(3-4):224-227.
[17] 赵要风,李宁,肖璐,等.猪FSHβ亚基基因结构区逆转座子插入突变及其与猪产仔数关系的研究[J].中国科学(C辑),1999,29(1):81-86. ZHAO Y F,LI N,XIAO L,et al.Studies on retrotransposition of transposon insertions and their relationship to the number of pigs born[J].Chinese Science (C series),1999,29(1):81-86.(in Chinese)
[18] 刘婵娟.猪ESR和FSHβ基因的遗传变异及其与繁殖性能关系的研究[D].泰安:山东农业大学,2007. LIU C J.Genetic variants of ESR and FSHβ gene and their association with reproduction traits in pigs[D].Tai’an:Shandong Agricultural University,2007.(in Chinese)
[19] MIHAILV N V,USATOV A V,GETMANTSEVA L V,et al.Associations between PRLR/Alu I gene polymorphism with reproductive growth and meat traits in pigs[J].Cytology and Genetics,2014,48(5):323-326.
[20] ZHAO Y F,LI N,XIAO L.FSHβ subunit gene is associated with major gene controlling litter size in commercial pig breeds[J].Science in China Series C-life Sciences,1998,41(6):664-668.
[21] 曾昭智,江涛,张锦红,等.西藏小型猪ESR基因Pvu Ⅱ多态性与生长繁殖性能相关分析[J].广东药学院学报,2009,25(2):192-195. ZENG Z Z,JIANG T,ZHANG J H,et al.Correlation analysis of the relationship between PvuⅡ polymorphisms of ESR gene and growth and reproduction traits of Tibet mini-pig[J].Journal of Guangdong Pharmaceutical College,2009,25(2):192-195.(in Chinese)
[22] 张淑君,熊远著,邓昌彦,等.大白母猪的ESR和PRLR二个基因位点多态性及其与产仔数相关性初探[J].华中农业大学学报,2001,20(1):11-14. ZHANG S J,XIONG Y Z,DENG C Y,et al.Study on the polymorphisms of ESR,PRLR and the relationship between polymorphism and litter size in Large White sows[J].Journal of Huazhong Agricultural University,2001,20(1):11-14.(in Chinese)
[23] 冯富彦,李新建,吕刚,等.豫南黑猪及3个引进猪种FSHβ和PRLR基因多态性与产仔性能的关系[J].华北农学报,2011,26(5):66-70. FENG F Y,LI X J,LYU G,et al.Relationship of genetic polymorphism of FSHβ and PRLR genes with litter size traits in Yunan Black pig and three foreign pig breeds[J].Acta Agriculturae Boreali-Sinica,2011,26(5):66-70.(in Chinese)
[24] 李小平,施启顺,柳小春,等.催乳素受体基因对大白、长白猪产仔数的影响[J].养猪,2005,6:19-20. LI X P,SHI Q S,LIU X C,et al.The effect of PRLR gene to litter size in Large White and Landrace pigs[J].Swine Production,2005,6:19-20.(in Chinese)
[25] WANG H T,CHEN L,JIANG Y W,et al.Association of gene polymorphisms of estrogen receptor follicle-stimulating hormone β and leptin with follicular cysts in Large White sows[J].Theriogenology,2017,103:143-148.
[26] 严隆盛,孙敬礼,王永庆,等.FSHβ和PRLR基因多态性对长白猪繁殖性能的影响[J].安徽农业科学,2009,37(30):15082-15083. YAN L S,SUN J L,WANG Y Q,et al.The influence of FSHβ and PRLR gene polymorphism on reproductive performance of Landrace sows[J].Anhui Agricultural University,2009,37(30):15082-15083.(in Chinese)
[27] HUNYADI B á,BALOGH P,NAGY K,et al.Association and polymorphism study of seven candidate genes with reproductive traits in three pig breeds in Hungary[J].Acta biochimica Polonica,2016,63(2):359-364.
[2] 刘燕.甘肃省猪种ESR和FSHβ基因多态性与繁殖性状的相关性研究[D].兰州:甘肃农业大学,2008. LIU Y.Study on the polymorphism of ESR,FSHβ and the association with reproduction traits in Gansu pigs[D].Lanzhou:Gansu Agricultural University,2008.(in Chinese)
[3] SCHENNINK A,TROFF J F,FREKING B A,et al.A novel first exon directs hormone-sensitive transcription of the pig prolactin receptor[J].Journal of Molecular Endocrinol,2013,51(1):1-13.
[4] 林长光,朱志明,李盛霖,等.猪PRLR基因Alu Ⅰ多态与繁殖性状相关性的研究[J].福建农业学报,2008,23(2):137-140. LIN C G,ZHU Z M,LI S L,et al.Relationship between Alu Ⅰ polymorphism of PRLR gene and reproduction trait of swine[J].Fujian Journal of Agricultural Sciences,2008,23(2):137-140.(in Chinese)
[5] 阮征,王春芳,韩艳云,等.以催乳素受体基因(PRLR)作为母猪产仔数候选基因的分析[J].上海畜牧兽医通讯,2002,1:20-21. RUAN Z,WANG C F,HAN Y Y,et al.Candidate gene analysis of the prolactin receptor gene (PRLR) for sow litter size[J].Shanghai Journal of Animal Husbandry and Veterinary Medicine,2002,1:20-21.(in Chinese)
[6] JENKINS Z A,HENRY H M,SIZE J A,et al.Follistatin (FST) growth hormone receptor (GHR) and prolactin receptor (PRLR) genes map to the same region of sheep chromosome 16[J].Animal Genetics,2000,31(4):28-31.
[7] BALATSKVI V N,SAIENKO A M,PENA R N,et al.The genetic diversity of pig breeding on ten production quantitative traits loci[J].Cytology and Genetics,2015,49(5):299-307.
[8] DROGEMULLER C,HAMANN H,DISTL O.Candidate gene markers for litter size in different German pig lines[J].Journal of Animal Science,2001,79(10):2565-2570.
[9] VINCENT A L,EVANS G,SHORT T H.The prolactin receptor gene is associated with increased litter size in pig[J].Swine Research Report,1998,27:15-18.
[10] ROTHSCHILD M F,VINCENT A L,TUGGLE C K,et al.A mutation in the prolactin receptor gene is associated with increased litter size in pigs[J].Animal Genetic,1998,29(1):69.
[11] LINVILLE R C,POMP D,JOHNSON R K,et al.Candidate gene analysis for loci affecting litter size and ovulation rate in swine[J].Animal Science,2001,79(1):60-67.
[12] LI P F,MENG J Z,JING J J,et al.Study on the relationship between expression patterns of cocaine-and amphetamine regulated transcript and hormones secretion in porcine ovarian follicles[J].Biological Research,2018,51(1):6.
[13] IVA A,GIOVANNI L,FRANCESCA M,et al.An in vitro prototype of a porcine biomimetic testis-like cell culture system:A novel tool for the study of reassembled Sertoli and Leydig cells[J].Asian Journal of Andrology,2017,20(2):160-165.
[14] 赵要风,李宁,陈永福,等.猪FSHβ亚基基因5′端调控区的PCR克隆测序及变异分析[J].自然科学进展(国家重点实验室通迅),1996,6(3):351-356. ZHAO Y F,LI N,CHEN Y F,et al.PCR cloning sequencing and mutation analysis of 5′-terminal regulatory region of pig FSHβ subunit gene[J].Natural Science Advances (National Key Laboratory Communications),1996,6(3):351-356.(in Chinese)
[15] 陈克飞,黄路生,李宁,等.猪FSHβ及ESR合并基因型对产仔数性状的影响[J].科学通报,2000,45(18):1963-1966. CHEN K F,HUANG L S,LI N,et al.Effect of pig FSHβ mode and ESR combinational genotype on the number of offspring produced[J].Scientific Report,2000,45(18):1963-1966.(in Chinese)
[16] MELLINK C,LANBIBM Y,YERLE M,et al.PCR amplification and physical localization of the genes for pig FSHβ and LHB[J].Cytogenetics and Cell Genetics,1995,70(3-4):224-227.
[17] 赵要风,李宁,肖璐,等.猪FSHβ亚基基因结构区逆转座子插入突变及其与猪产仔数关系的研究[J].中国科学(C辑),1999,29(1):81-86. ZHAO Y F,LI N,XIAO L,et al.Studies on retrotransposition of transposon insertions and their relationship to the number of pigs born[J].Chinese Science (C series),1999,29(1):81-86.(in Chinese)
[18] 刘婵娟.猪ESR和FSHβ基因的遗传变异及其与繁殖性能关系的研究[D].泰安:山东农业大学,2007. LIU C J.Genetic variants of ESR and FSHβ gene and their association with reproduction traits in pigs[D].Tai’an:Shandong Agricultural University,2007.(in Chinese)
[19] MIHAILV N V,USATOV A V,GETMANTSEVA L V,et al.Associations between PRLR/Alu I gene polymorphism with reproductive growth and meat traits in pigs[J].Cytology and Genetics,2014,48(5):323-326.
[20] ZHAO Y F,LI N,XIAO L.FSHβ subunit gene is associated with major gene controlling litter size in commercial pig breeds[J].Science in China Series C-life Sciences,1998,41(6):664-668.
[21] 曾昭智,江涛,张锦红,等.西藏小型猪ESR基因Pvu Ⅱ多态性与生长繁殖性能相关分析[J].广东药学院学报,2009,25(2):192-195. ZENG Z Z,JIANG T,ZHANG J H,et al.Correlation analysis of the relationship between PvuⅡ polymorphisms of ESR gene and growth and reproduction traits of Tibet mini-pig[J].Journal of Guangdong Pharmaceutical College,2009,25(2):192-195.(in Chinese)
[22] 张淑君,熊远著,邓昌彦,等.大白母猪的ESR和PRLR二个基因位点多态性及其与产仔数相关性初探[J].华中农业大学学报,2001,20(1):11-14. ZHANG S J,XIONG Y Z,DENG C Y,et al.Study on the polymorphisms of ESR,PRLR and the relationship between polymorphism and litter size in Large White sows[J].Journal of Huazhong Agricultural University,2001,20(1):11-14.(in Chinese)
[23] 冯富彦,李新建,吕刚,等.豫南黑猪及3个引进猪种FSHβ和PRLR基因多态性与产仔性能的关系[J].华北农学报,2011,26(5):66-70. FENG F Y,LI X J,LYU G,et al.Relationship of genetic polymorphism of FSHβ and PRLR genes with litter size traits in Yunan Black pig and three foreign pig breeds[J].Acta Agriculturae Boreali-Sinica,2011,26(5):66-70.(in Chinese)
[24] 李小平,施启顺,柳小春,等.催乳素受体基因对大白、长白猪产仔数的影响[J].养猪,2005,6:19-20. LI X P,SHI Q S,LIU X C,et al.The effect of PRLR gene to litter size in Large White and Landrace pigs[J].Swine Production,2005,6:19-20.(in Chinese)
[25] WANG H T,CHEN L,JIANG Y W,et al.Association of gene polymorphisms of estrogen receptor follicle-stimulating hormone β and leptin with follicular cysts in Large White sows[J].Theriogenology,2017,103:143-148.
[26] 严隆盛,孙敬礼,王永庆,等.FSHβ和PRLR基因多态性对长白猪繁殖性能的影响[J].安徽农业科学,2009,37(30):15082-15083. YAN L S,SUN J L,WANG Y Q,et al.The influence of FSHβ and PRLR gene polymorphism on reproductive performance of Landrace sows[J].Anhui Agricultural University,2009,37(30):15082-15083.(in Chinese)
[27] HUNYADI B á,BALOGH P,NAGY K,et al.Association and polymorphism study of seven candidate genes with reproductive traits in three pig breeds in Hungary[J].Acta biochimica Polonica,2016,63(2):359-364.