激光诱导小鼠视网膜形态变化的实验研究
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摘要
目的探讨不同功率的激光对C57BL/6J小鼠视网膜形态变化的影响。方法选择6～8周龄的雄性C57BL/6J小鼠40只,体重25～30 g,经直接检眼镜及裂隙灯显微镜检查眼部情况均正常,分成对照组和激光组(一组100 mw,二组150 mw,三组200 mw)每组各10只小鼠。对照组不做任何处理,激光组小鼠用复方托比卡胺散瞳后,设定激光波长532 nm,光斑直径50μm,曝光时间0.1 s,用不同功率激光在距离小鼠视盘1～2 PD位置进行光凝,每只眼做2～3个光凝点,分别于光凝后1周和2周行眼底照相、OCT及FFA检查,观察眼底变化情况。结果激光功率为100 mw时,眼底照相检查可见激光斑为淡灰色,FFA检查激光斑处未见明显渗漏,OCT检查可见色素上皮层未见明显损害,视网膜神经纤维层损伤。激光功率为150 mw时,眼底照相检查可见激光斑呈雾状灰色混浊,FFA检查可见激光斑处荧光渗漏,OCT检查示视网膜RPE层不连续,视网膜神经纤维层下有高反射光团出现,随着时间推移表现加重。激光功率为200 mw时,眼底照相示激光斑呈灰白色,FFA检查显示激光斑处及周围呈现高荧光,晚期荧光明显增强,OCT检查显示:视网膜囊样水肿,神经纤维层下出现团状高反射区。结论激光功率设定为200 mw,光斑直径为50μm,波长532 nm,曝光时间0.1 s时可以形成较典型的湿性AMD动物模型。
Objective To explore the effect of different laser power on retinal morphology of C57 BL/6 J mice. Methods 40 male C57 BL/6 J mice, aged 6～8 weeks, weighing 25～30 g, were examined by direct ophthalmoscope and slit lamp microscope. They were divided into two kinds of groups: the control group and the laser groups(the first group was100 mw, the second group was 150 mw and the third group was 200 mw). There were 10 mice in each group. No treatment was given in the control group. The optical maser wavelength was 532 nm, the spot diameter was 50 μm, the exposure time was 0.1 s in the laser groups after dilating pupil with compound tobikamide. The mice in the laser groups were photocoagulated with different power laser at the 1～2 PD position, each eye was given 2～3 light condensation points. Fundus photography, OCT and FFA were performed to check fundus changes one week and two weeks after photocoagulation. Results When laser power was 100 mw, laser spot was light gray in fundus radiography, leakage wasnot found in laser spot by FFA,no obvious damage was found in retinal pigment epithelium by OCT, but retinal nerve fiber layer was injured. When the laser power was 150 mw, the laser spot is foggy with gray in fundus photography, fluorescence leakage was found in laser spot by FFA, the retinal pigment epithelium is discontinuous by OCT and there was a high reflection light mass under the retinal nerve fiber layer and light mass increaseed over time. When the laser power was 200 mw, fundus photography showed that the laser spot was gray white, FFA showed high fluorescence at and around the laser spot, the late fluorescence was obviously enhanced, and the OCT showed that the retina had cystic edema and there was a high reflex area under the nerve fiber layer. Conclusion When the laser power is 200 mw, the spot diameter is 50 μm, the wavelength is 532 nm and the exposure time is 0.1 s, a typical wet AMD animal model was formed.
Objective To explore the effect of different laser power on retinal morphology of C57 BL/6 J mice. Methods 40 male C57 BL/6 J mice, aged 6～8 weeks, weighing 25～30 g, were examined by direct ophthalmoscope and slit lamp microscope. They were divided into two kinds of groups: the control group and the laser groups(the first group was100 mw, the second group was 150 mw and the third group was 200 mw). There were 10 mice in each group. No treatment was given in the control group. The optical maser wavelength was 532 nm, the spot diameter was 50 μm, the exposure time was 0.1 s in the laser groups after dilating pupil with compound tobikamide. The mice in the laser groups were photocoagulated with different power laser at the 1～2 PD position, each eye was given 2～3 light condensation points. Fundus photography, OCT and FFA were performed to check fundus changes one week and two weeks after photocoagulation. Results When laser power was 100 mw, laser spot was light gray in fundus radiography, leakage wasnot found in laser spot by FFA,no obvious damage was found in retinal pigment epithelium by OCT, but retinal nerve fiber layer was injured. When the laser power was 150 mw, the laser spot is foggy with gray in fundus photography, fluorescence leakage was found in laser spot by FFA, the retinal pigment epithelium is discontinuous by OCT and there was a high reflection light mass under the retinal nerve fiber layer and light mass increaseed over time. When the laser power was 200 mw, fundus photography showed that the laser spot was gray white, FFA showed high fluorescence at and around the laser spot, the late fluorescence was obviously enhanced, and the OCT showed that the retina had cystic edema and there was a high reflex area under the nerve fiber layer. Conclusion When the laser power is 200 mw, the spot diameter is 50 μm, the wavelength is 532 nm and the exposure time is 0.1 s, a typical wet AMD animal model was formed.
引文
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[2] Hodgson R, Reason T, Trueman D, et al. Challenges associated with estimating utility in wet age-related macular degeneration:A novel regression analysis to capture the bilateral nature of the disease[J].Advances In Therapy,2017,34(10):2360-2370.
[3] Lim L S,Mitchell P,Seddon J M, et al.Age-related macular degenera tion[J]. Lancet, 2012,379(9827):1728-1738.
[4] Mehta S. Age-related macular degeneration[J].Prim care 2015,42(3):377-391.
[5] Sakimoto S,Sakaguchi H,Ohji M,et al.Consecutive case series with long-term follow-up of full macular translocation for myopic choroidal neovascularisation[J].Br J Ophthalmol, 2014,98(9):1221-1225.
[6] Hoerster R,Muether P S,Vierkotten S,et al.In-vivo and ex-vivo characterization of laser-induced choroidal neovascularization variability in mice[J].Graefes Arch Clin Exp Ophthalmol,2012,250(11):1579-1586.
[7] Giani A,Thanos A,Roh M I,et al.In vivo evaluation of laser-induced choroidal neovascularization using spectral-domain optical coherence tomography[J]. Invest Ophthalmol Vis Sci, 2011,52(6):3880-3887.
[8] Shah R S,Soetikno B T,Lajko M,et al. A mouse model for laser-induced choroidal neovascularization[J]. Vis Exp, 2015,(106):53502.
[9]张明,夏庆杰,张军军,等.激光诱导弥猴眼脉络膜新生血管动物模型[A],第三届全球华人眼科学术大会暨中华医学会第十一届全国眼科学术大会论文汇编[C].2006.
[10]李婷,宋艳.荧光素眼底血管造影评估雷珠单抗治疗渗出型年龄相关性黄斑变性的疗效及其与视力相关性的研究[J].中华眼科医学杂志,2016,6(1):31-35.
[11]魏丽丽.眼底荧光血管造影在年龄相关性黄斑变性患者中的临床应用价值[J].江苏医药,2014,40(12):1470-1471.
[12] Liu T,Hui L,Wang Y S,et al. In-vivo investigation of laser-induced choroidal neovascularization in rat using spectaral-domain optical coherence tomography(SD-OCT)[J].Graefes Arch Clin Exp Ophthalmol,2013,251(5):1293-1301.
[13] Pron G.Optical coherence tomography monitoring strategies for AVEGF-treated age-related macular degeneration:an evidencebased analysis[J].Ant Health Technol Assess Ser,2014,14(10):1-64.
[14] PhilipJ.Rosenfeld.Optical coherence tomography and the development of antiangiogenic therapies in neovascular age-related macular degeneration[J].Invest Ophthalmol Vis Sci, 2016, 57(9):14-26.