大麦白粉病抗性的遗传分析与QTL定位
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摘要
为探究大麦白粉病抗性遗传,定位其抗性QTL,本研究以抗病品种Gairdner和感病品种扬饲麦1号杂交F1花药培养构建的DH群体及亲本为材料,对大麦白粉病抗性进行鉴定与遗传分析,并利用91对在亲本间多态性好的SSR标记构建了群体的遗传连锁图谱,采用Windows QTL IciMapping 4. 0软件中的完备区间-加性模型对大麦白粉病抗性QTL进行定位。结果表明,DH群体各系间存在丰富的大麦白粉病抗性遗传变异。共检测到5个与大麦白粉病抗性相关的QTLs。其中3个时期均检测到qPM-2Ha位于Bmag0711-AWBMS56区间,可解释的表型变异为7. 48%～12. 50%; qPM-4Ha位于EBmac0906-HVM68区间,可解释的表型变异为23. 07%～32. 09%; 2个时期均检测到qPM-2Hb位于Bmag0749-GBM1475区间,可解释的表型变异为6. 22%～8. 13%。qPM-2Ha、qPM-4Ha及qPM-2Hb白粉病抗性基因均来源于抗病亲本Gairdner,qPM-3Ha和qPM-4Hb白粉病抗性基因来源于感病亲本扬饲麦1号,qPM-2Hb和qPM-3Ha可能是2个新的大麦白粉病抗性QTLs位点。本研究结果为大麦白粉病抗性基因的发掘、精细定位、克隆及分子标记辅助选择育种奠定了基础。
In order to explore the inheritance of resistance to barley powdery mildew and locate its resistance QTLs,in the present study,a total of 173 doubled-haploid( DH) lines generated from a cross between a disease-resistant cultivar Gairdner and a susceptible Chinese landrace barley Yangsimai 1 were used to identify the resistance to barley powdery mildew and andyzed its genetic characteristics. A genetic linkage map including 91 pairs of SSR markers with obvious polymorphism between the parents was constructed. QTL mapping for barley powdery mildew was carried out by using the complete interval-additive model( Inclusive Composite Interval-Additive Mapping,ICIM-ADD) method in Windows QTL IciMapping 4. 0 software. The results showed that there were abundant genetic variations of barley powdery mildew resistance in the DH population. Five QTLs for the disease index of barley powdery mildew were identified,among which two QTLs( qPM-2 Ha and qPM-4 Ha) were detected in all three stages. qPM-2 Ha was located in the Bmag0711 ～AWBMS56 interval,explained 7. 48% ～ 12. 50% of barley powdery mildew resistant variation; qPM-4 Ha was located in the EBmac0906 ～ HVM68 interval,explained 23. 07% ～ 32. 09% of barley powdery mildew resistant variation; qPM-2 Hb was detected in the first and second stages,located in the Bmag0749 ～ GBM1475 interval,explained 6. 22% ～8. 13% of barley powdery mildew resistant variation. qPM-2 Ha,qPM-4 Ha and qPM-2 Hb that enhance the resistance to powdery mildew were derived from Gairdner,the resistant parent,while qPM-3 Ha and qPM-4 Hb were derived from the susceptible parent Yangsimai 1. Two putative QTLs,qPM-2 Hb and qPM-3 Ha likely represent new loci for barley powdery mildew resistance. The results have laid a foundation for further exploration,fine mapping,gene cloning and molecular-marker-assisted selection breeding of barley powdery mildew.
In order to explore the inheritance of resistance to barley powdery mildew and locate its resistance QTLs,in the present study,a total of 173 doubled-haploid( DH) lines generated from a cross between a disease-resistant cultivar Gairdner and a susceptible Chinese landrace barley Yangsimai 1 were used to identify the resistance to barley powdery mildew and andyzed its genetic characteristics. A genetic linkage map including 91 pairs of SSR markers with obvious polymorphism between the parents was constructed. QTL mapping for barley powdery mildew was carried out by using the complete interval-additive model( Inclusive Composite Interval-Additive Mapping,ICIM-ADD) method in Windows QTL IciMapping 4. 0 software. The results showed that there were abundant genetic variations of barley powdery mildew resistance in the DH population. Five QTLs for the disease index of barley powdery mildew were identified,among which two QTLs( qPM-2 Ha and qPM-4 Ha) were detected in all three stages. qPM-2 Ha was located in the Bmag0711 ～AWBMS56 interval,explained 7. 48% ～ 12. 50% of barley powdery mildew resistant variation; qPM-4 Ha was located in the EBmac0906 ～ HVM68 interval,explained 23. 07% ～ 32. 09% of barley powdery mildew resistant variation; qPM-2 Hb was detected in the first and second stages,located in the Bmag0749 ～ GBM1475 interval,explained 6. 22% ～8. 13% of barley powdery mildew resistant variation. qPM-2 Ha,qPM-4 Ha and qPM-2 Hb that enhance the resistance to powdery mildew were derived from Gairdner,the resistant parent,while qPM-3 Ha and qPM-4 Hb were derived from the susceptible parent Yangsimai 1. Two putative QTLs,qPM-2 Hb and qPM-3 Ha likely represent new loci for barley powdery mildew resistance. The results have laid a foundation for further exploration,fine mapping,gene cloning and molecular-marker-assisted selection breeding of barley powdery mildew.
引文
[1]Hua W,Zhang X Q,Zhu J H,Yang J M.A study of genetic diversity of colored barley(Hordeum vulgare L.)using SSR markers[J].Genetic Resources and Crop Evolution,2015,62(3):395-406
[2]栾海业,臧慧,沈会权,张英虎,乔海龙,陶红,陈健,陈和.大麦白化颖壳突变体的转录组学分析[J].核农学报,2017,31(12):2332-2339
[3]Jensen H R,Dreiseitl A,Sadiki M,Schoen D J.High diversity,low spatial structure and rapid pathotype evolution in Moroccan populations of Blumeria graminis f.sp.hordei[J].European Journal of Plant Pathology,2013,136(2):323-336
[4]Zhu J H,Wang J M,Jia Q J,Yang J M,Zhou Y J,Lin F,Hua W,Shang Y.Pathotypes and genetic diversity of Blumeria graminis f.sp.hordei in the winter barley regions in China[J].Journal of Integrative Agriculture,2010,9(12):1787-1798
[5]陈升位,王楠,陈疏影,刘新友,吴细权,郭华春.不同大麦品种的白粉病抗性鉴定和生态适应性评价[J].麦类作物学报,2013,33(3):600-603
[6]黄金堂,郭媛贞,陈德禄,许国诚,陈炳坤.我国栽培大麦白粉病抗性特点分析[J].麦类作物学报,2002,22(1):80-83
[7]Backes G,Madsen L H,Jaiser H,Stougaard J,Herz M,MohlerⅤ,Jahoor A.Localisation of genes for resistance against Blumeria graminis f.sp.hordei and Puccinia graminis in a cross between a barley cultivar and a wild barley(Hordeum vulgare ssp.spontaneum)line[J].Theoretical and Applied Genetics,2003,106(2):353-362
[8]Giese H.Powdery mildew resistance genes in the Mla and Mlk regions on barley chromosome 5[J].Hereditas,2009,95(1):51-62
[9]严隽析.大麦对大麦白粉病菌抗性遗传的初步研究[J].植物病理学报,1996(1):24-24
[10]俞志隆,黄培忠.大麦遗传与改良[M].上海:上海科学技术出版社,1994:321-334
[11]黄金堂.大麦白粉病抗性的遗传分析[J].麦类作物学报,2011,31(1):35-40
[12]Chekowski J,Tyrka M,Sobkiewicz A.Resistance genes in barley(Hordeum vulgare L.)and their identification with molecular markers[J].Journal of Applied Genetics,2003,44(3):291-309
[13]Weibull J,Walther U,Sato K,HabekuA,Kopahnke D,Proeseler G.Chapter 8 Diversity in Resistance to Biotic Stresses[M].Japan:Developments in Plant Genetics and Breeding,2003:143-178
[14]韩德俊,李振岐,曹莉,陈耀锋.大麦抗白粉病基因Mlo的研究进展[J].西北植物学报,2003,23(3):496-502
[15]朱靖环,周益军,杨建明.大麦白粉病菌遗传学研究进展[J].植物保护学报,2014,41(1):109-117
[16]Li H B,Zhou M X.Quantitative trait loci controlling barley powdery mildew and scald resistances in two different barley doubled haploid populations[J].Molecular Breeding,2011,27(4):479-490
[17]王建康.数量性状基因的完备区间作图方法[J].作物学报,2009,35(2):239-245
[18]李慧慧,张鲁燕,王建康.数量性状基因定位研究中若干常见问题的分析与解答[J].作物学报,2010,36(6):918-931
[19]Mc Couch S R,Cho Y G,Yano P E,Blinstrub M,Morishima H,Kinoshita T.Report on QTL nomenclature[J].Rice Genet Newslett,1997,11(14):11-13
[20]李韬.小麦白粉病小种特异抗性新基因Pm-M53的遗传分析[D].扬州:扬州大学,2005
[21]陈厚德,王彰明,李清哈,华松林.大麦白粉菌无性阶段生物学特性研究[J].大麦与谷类科学,1987(4):30-37
[22]杜娟,王建军,曾亚文,杨涛,普晓英,杨树明.高密度高氮肥对大麦白粉病抗病性及产量性状的影响[J].浙江农业学报,2011,23(1):117-121
[23]Heun M.Mapping quantitative powdery mildew resistance of barley using a restriction fragment length polymorphism map[J].Genome,2011,35(6):1019-1025
[24]Silvar C,Dhif H,Igartua E,Kopahnke D,Gracia M P,Lasa J M,Ordon F,Casas A M.Identification of quantitative trait loci for resistance to powdery mildew in a Spanish barley landrace[J].Molecular Breeding,2010,25(4):581-592
[25]Büschges R,Hollricher K,Panstruga R,Simons G,Wolter M,Frijters A,van Daelen R,van der Lee T,Diergaarde P,Groenendijk J,T9psch S,Vos P,Salamini F,Schulze-Lefert P.The barley Mlo gene:a novel control element of plant pathogen resistance[J].Cell,1997,88(5):695-705
[26]Halterman D,Zhou F,Wei F,Wise R P,Schulze-Lefert P.The MLA6 coiled-coil,NBS-LRR protein confers Avr Mla6-dependent resistance specificity to Blumeria graminis f.sp.hordei in barley and wheat[J].Plant Journal,2001,25(3):335-348
[27]黄金堂,李清华,陈海玲.大麦种质资源白粉病抗性鉴定与应用[J].植物遗传资源学报,2008,9(1):101-104
[28]Zhou F,Schulze-Lefert P.Cell-autonomous expression of barley Mla1 confers race-specific resistance to the powdery mildew fungus via a Rar1-independent signaling pathway[J].Plant Cell,2001,13(2):337-350
[29]Hilbers S,Fischbeck G,Jahoor A.Localization of the Laevigatum resistance gene Ml La against powdery mildew in the barley genome by the use of RFLP markers[J].Plant Breeding,1992,109(4):335-338
[30]G9rg R,Hollricher K,Schulze-Lefert P.Functional analysis and RFLP-mediated mapping of the Mlg resistance locus in barley[J].Plant Journal,1993,3(6):857-866
[31]Hinze K,Schulze-Lefert P.Restriction fragment length polymorphism-mediated targeting of the mlo resistance locus in barley(Hordeum vulgare L.)[J].Proceedings of the National Academy of Sciences of the United States of America,1991,88(9):3691-3695
[2]栾海业,臧慧,沈会权,张英虎,乔海龙,陶红,陈健,陈和.大麦白化颖壳突变体的转录组学分析[J].核农学报,2017,31(12):2332-2339
[3]Jensen H R,Dreiseitl A,Sadiki M,Schoen D J.High diversity,low spatial structure and rapid pathotype evolution in Moroccan populations of Blumeria graminis f.sp.hordei[J].European Journal of Plant Pathology,2013,136(2):323-336
[4]Zhu J H,Wang J M,Jia Q J,Yang J M,Zhou Y J,Lin F,Hua W,Shang Y.Pathotypes and genetic diversity of Blumeria graminis f.sp.hordei in the winter barley regions in China[J].Journal of Integrative Agriculture,2010,9(12):1787-1798
[5]陈升位,王楠,陈疏影,刘新友,吴细权,郭华春.不同大麦品种的白粉病抗性鉴定和生态适应性评价[J].麦类作物学报,2013,33(3):600-603
[6]黄金堂,郭媛贞,陈德禄,许国诚,陈炳坤.我国栽培大麦白粉病抗性特点分析[J].麦类作物学报,2002,22(1):80-83
[7]Backes G,Madsen L H,Jaiser H,Stougaard J,Herz M,MohlerⅤ,Jahoor A.Localisation of genes for resistance against Blumeria graminis f.sp.hordei and Puccinia graminis in a cross between a barley cultivar and a wild barley(Hordeum vulgare ssp.spontaneum)line[J].Theoretical and Applied Genetics,2003,106(2):353-362
[8]Giese H.Powdery mildew resistance genes in the Mla and Mlk regions on barley chromosome 5[J].Hereditas,2009,95(1):51-62
[9]严隽析.大麦对大麦白粉病菌抗性遗传的初步研究[J].植物病理学报,1996(1):24-24
[10]俞志隆,黄培忠.大麦遗传与改良[M].上海:上海科学技术出版社,1994:321-334
[11]黄金堂.大麦白粉病抗性的遗传分析[J].麦类作物学报,2011,31(1):35-40
[12]Chekowski J,Tyrka M,Sobkiewicz A.Resistance genes in barley(Hordeum vulgare L.)and their identification with molecular markers[J].Journal of Applied Genetics,2003,44(3):291-309
[13]Weibull J,Walther U,Sato K,HabekuA,Kopahnke D,Proeseler G.Chapter 8 Diversity in Resistance to Biotic Stresses[M].Japan:Developments in Plant Genetics and Breeding,2003:143-178
[14]韩德俊,李振岐,曹莉,陈耀锋.大麦抗白粉病基因Mlo的研究进展[J].西北植物学报,2003,23(3):496-502
[15]朱靖环,周益军,杨建明.大麦白粉病菌遗传学研究进展[J].植物保护学报,2014,41(1):109-117
[16]Li H B,Zhou M X.Quantitative trait loci controlling barley powdery mildew and scald resistances in two different barley doubled haploid populations[J].Molecular Breeding,2011,27(4):479-490
[17]王建康.数量性状基因的完备区间作图方法[J].作物学报,2009,35(2):239-245
[18]李慧慧,张鲁燕,王建康.数量性状基因定位研究中若干常见问题的分析与解答[J].作物学报,2010,36(6):918-931
[19]Mc Couch S R,Cho Y G,Yano P E,Blinstrub M,Morishima H,Kinoshita T.Report on QTL nomenclature[J].Rice Genet Newslett,1997,11(14):11-13
[20]李韬.小麦白粉病小种特异抗性新基因Pm-M53的遗传分析[D].扬州:扬州大学,2005
[21]陈厚德,王彰明,李清哈,华松林.大麦白粉菌无性阶段生物学特性研究[J].大麦与谷类科学,1987(4):30-37
[22]杜娟,王建军,曾亚文,杨涛,普晓英,杨树明.高密度高氮肥对大麦白粉病抗病性及产量性状的影响[J].浙江农业学报,2011,23(1):117-121
[23]Heun M.Mapping quantitative powdery mildew resistance of barley using a restriction fragment length polymorphism map[J].Genome,2011,35(6):1019-1025
[24]Silvar C,Dhif H,Igartua E,Kopahnke D,Gracia M P,Lasa J M,Ordon F,Casas A M.Identification of quantitative trait loci for resistance to powdery mildew in a Spanish barley landrace[J].Molecular Breeding,2010,25(4):581-592
[25]Büschges R,Hollricher K,Panstruga R,Simons G,Wolter M,Frijters A,van Daelen R,van der Lee T,Diergaarde P,Groenendijk J,T9psch S,Vos P,Salamini F,Schulze-Lefert P.The barley Mlo gene:a novel control element of plant pathogen resistance[J].Cell,1997,88(5):695-705
[26]Halterman D,Zhou F,Wei F,Wise R P,Schulze-Lefert P.The MLA6 coiled-coil,NBS-LRR protein confers Avr Mla6-dependent resistance specificity to Blumeria graminis f.sp.hordei in barley and wheat[J].Plant Journal,2001,25(3):335-348
[27]黄金堂,李清华,陈海玲.大麦种质资源白粉病抗性鉴定与应用[J].植物遗传资源学报,2008,9(1):101-104
[28]Zhou F,Schulze-Lefert P.Cell-autonomous expression of barley Mla1 confers race-specific resistance to the powdery mildew fungus via a Rar1-independent signaling pathway[J].Plant Cell,2001,13(2):337-350
[29]Hilbers S,Fischbeck G,Jahoor A.Localization of the Laevigatum resistance gene Ml La against powdery mildew in the barley genome by the use of RFLP markers[J].Plant Breeding,1992,109(4):335-338
[30]G9rg R,Hollricher K,Schulze-Lefert P.Functional analysis and RFLP-mediated mapping of the Mlg resistance locus in barley[J].Plant Journal,1993,3(6):857-866
[31]Hinze K,Schulze-Lefert P.Restriction fragment length polymorphism-mediated targeting of the mlo resistance locus in barley(Hordeum vulgare L.)[J].Proceedings of the National Academy of Sciences of the United States of America,1991,88(9):3691-3695