水族抗痹症药酒动物急性毒性试验
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摘要
目的:研究水族抗痹症药酒的急性毒性,确定其安全范围。方法:抗痹症药酒及对照白酒不同浓度小鼠一次灌胃给药,观察7 d内小鼠死亡数,Bliss法测定小鼠的LD_(50)。结果:抗痹症药酒小鼠一次经口给药LD_(50)=29.664ml/kg,LD_(50)的95%可信限为26.452~31.904ml/kg;白酒对照组小鼠一次经口给药LD_(50)=27.222 ml/kg,LD_(50)的95%的可信限=24.194~29.759ml/kg。结论:抗痹症药酒临床建议成人适用量23.4ml/d,服用安全。
Objective: To study the acute toxicity of aquatic anti-arthralgia medicinal liquor and determine its safety scope. Methods: Mice with different concentrations of aquatic anti-arthralgia medicinal liquor and controlled liquor were administered intragastrically once a day,and the number of mice died within 7 days was observed. The LD_(50) of mice was determined by Bliss method. Result: The one-dose oral administration of aquatic anti-arthralgia medicinal liquor LD_(50) was 29.664ml/kg,the 95% confidence limit of LD_(50) was26.452 ~ 31.904ml/kg. In the control group of white wine,one dose administration LD_(50) was 27.222ml/kg,the 95% confidence limit of LD_(50)= 24.194 ~ 29.759 ml/kg. Conclusion: The clinical application of aquatic anti-arthralgia medicinal liquor is recommended to be 23.4ml/d for adult use.
Objective: To study the acute toxicity of aquatic anti-arthralgia medicinal liquor and determine its safety scope. Methods: Mice with different concentrations of aquatic anti-arthralgia medicinal liquor and controlled liquor were administered intragastrically once a day,and the number of mice died within 7 days was observed. The LD_(50) of mice was determined by Bliss method. Result: The one-dose oral administration of aquatic anti-arthralgia medicinal liquor LD_(50) was 29.664ml/kg,the 95% confidence limit of LD_(50) was26.452 ~ 31.904ml/kg. In the control group of white wine,one dose administration LD_(50) was 27.222ml/kg,the 95% confidence limit of LD_(50)= 24.194 ~ 29.759 ml/kg. Conclusion: The clinical application of aquatic anti-arthralgia medicinal liquor is recommended to be 23.4ml/d for adult use.
引文
[1]陈奇.中药药理研究方法学[M].北京:人民卫生出版社,1993:112-118.
[2]徐叔云,卞如濂,陈修.药理实验方法学[M].3版.北京:人民卫生出版社,2002:227-229.
[3]刘毓谷.卫生毒理学基础[M].北京:人民卫生出版社,1987:91.
[4]王燕林,梁文妹,李容2),等.某54度食用白酒对大鼠肝细胞TNF-α表达及凋亡的影响[J].贵州医科大学学报,2017,42(6):661-665.
[2]徐叔云,卞如濂,陈修.药理实验方法学[M].3版.北京:人民卫生出版社,2002:227-229.
[3]刘毓谷.卫生毒理学基础[M].北京:人民卫生出版社,1987:91.
[4]王燕林,梁文妹,李容2),等.某54度食用白酒对大鼠肝细胞TNF-α表达及凋亡的影响[J].贵州医科大学学报,2017,42(6):661-665.