羧甲基茯苓多糖的制备及抗肿瘤活性研究
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摘要
目的提取茯苓药材中的多糖,测定其总糖含量,并进行羧甲基化改性,考察茯苓多糖与修饰后的羧甲基茯苓多糖的抗肿瘤活性。方法采用稀碱浸提法提取茯苓多糖,苯酚-硫酸显色法测定茯苓多糖中总糖含量,通过羧甲基化反应得到羧甲基茯苓多糖。并采用MTT法检测茯苓多糖与羧甲基茯苓多糖对人肝癌细胞HepG-2的毒性反应。结果苯酚-硫酸法测得茯苓多糖中总糖含量为95.96%,羧甲基茯苓多糖与茯苓多糖对HepG-2细胞的增殖均具有一定的抑制作用,其中羧甲基茯苓多糖活性更强。结论经过修饰后的羧甲基茯苓多糖具有更好的抗肿瘤活性。
OBJECTIVE To extract pachyman polysaccharides(PPS) from Poria cocos, and determine the content of total polysaccharide in PPS. To obtain the carboxymethylpachymaran(CMP) by carboxymethylation modification of PPS, and evaluate the antitumor activity of PPS and CMP. METHODS Soaking extraction by diluted alkali solution was used to prepare PPS, and phenol-sulfuric method was used to measure the total polysaccharide. Preparation of CMP by carboxymethylation reaction. MTT assay was used to determine the cytotoxicity of PPS and CMP for HepG-2. RESULTS The content of the total polysaccharide in PPS was 95.96%, which determined by phenol-sulfuric method. Both of PPS and CMP inhibited the proliferation of HepG-2 cells, CMP had stronger activity. CONCLUSION The anti-tumor activity of CMP, which was modified with carboxymethyl, is more prominent than PPS.
OBJECTIVE To extract pachyman polysaccharides(PPS) from Poria cocos, and determine the content of total polysaccharide in PPS. To obtain the carboxymethylpachymaran(CMP) by carboxymethylation modification of PPS, and evaluate the antitumor activity of PPS and CMP. METHODS Soaking extraction by diluted alkali solution was used to prepare PPS, and phenol-sulfuric method was used to measure the total polysaccharide. Preparation of CMP by carboxymethylation reaction. MTT assay was used to determine the cytotoxicity of PPS and CMP for HepG-2. RESULTS The content of the total polysaccharide in PPS was 95.96%, which determined by phenol-sulfuric method. Both of PPS and CMP inhibited the proliferation of HepG-2 cells, CMP had stronger activity. CONCLUSION The anti-tumor activity of CMP, which was modified with carboxymethyl, is more prominent than PPS.
引文
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[4]WASSER S P.Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides[J].Appl Microbiol Biotechnol,2002,60(3):258-274.
[5]CHIHARA G,MAEDA Y,HAMURO J,et al.Inhibition of mouse sarcoma 180 by polysaccharides from Lentinus edodes(Berk.)sing[J].Nature,1969,222(5194):687-688.
[6]WEI X J,HU T J,CHEN J R,et al.Inhibitory effect of carboxymethylpachymaran on cyclophosphamide-induced oxidative stress in mice[J].Int J Biol Macromol,2011,49(4):801-805.
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