益消方对自发性糖尿病模型KKAy小鼠认知功能影响的Morris水迷宫观察
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摘要
目的:探讨益消方对糖尿病KKAy小鼠学习记忆行为的影响。方法:24只雄性KKAy小鼠随机分为模型组及中药组,12只雄性C57BL/6J小鼠为正常组。中药组予以中药复方益消方灌服,模型组及正常组予灌服等量蒸馏水,在灌胃4周和8周后分别进行Morris水迷宫观察。采用Morris水迷宫图像自动采集和软件分析系统记录逃避潜伏期、游泳轨迹、圆台穿越次数、初始角度等。结果:定位航行试验中,与正常组比较,灌胃4周、8周后模型组逃避潜伏期均明显延长,差异有统计学意义(P <0. 05);灌胃8周后,中药组逃避潜伏期在训练第4、5天时与模型组比较明显缩短,差异有统计学意义(P <0. 05)。空间探索试验中,灌胃8周后模型组目标象限游泳时间占总时间百分比、圆台穿越次数较4周后有所减少,且与正常组及中药组差距增加,初始角度与正常组比较明显增加,差异有统计学意义(P <0. 05)。结果显示益消方缩短糖尿病动物模型小鼠逃避潜伏期,增加原圆台穿越次数及目标象限游泳时间。结论:益消方具有一定延缓糖尿病小鼠认知功能障碍发生进程的功效,改善其学习记忆行为。
Objective: To observe the improve effects of traditional Chinese medicine( TCM) Yixiao Decoction on learning,memory behavior of the KKAy mice. Methods: A total of 24 male KKAy mice were randomly divided into model group and TCM group.A total of 12 male C57 BL/6 J mice were normal group. The mice of different groups were treated with relevant medicinal respectively by intragastrical method,as the TCM group were fed with Yixiao Decoction. The model group and normal group were fed with distilled water. All groups of mice were performed Morris water maze behavior test respectively when the finish of gavage for 4 and 8 weeks. The Morris water maze's tracking and analytic system of the experiential animal trace was adopted to record the escape latency,the swimming trajectory of the space exploration test,times across the platform and initial angle,etc. Results: Compared with the normal group,the escape latency of the model group was obviously increased at 4 and 8 weeks after intragastric administration( P < 0. 05 =. And the Chinese medicine group was shorter than that of the model group on the 4 th and 5 th day after 8 weeks of gastric administration( P < 0. 05 =. In the space exploration test,the percentage of time in target quadrant and the crossing times of model group decreased after 8 weeks in contrast with 4 weeks. The difference between the model group and normal group,traditional Chinese medicine group was increased and the initial angle was significantly increased compared with normal group( P < 0. 05 =.The results showed that Yixiao Decoction could shorten the escape latency of diabetic mice,increase the number of times of traversing the original platform and swimming time in the target quadrant. Conclusion: Yixiao Decoction has the effects of delaying the occurrence of cognitive impairment in diabetic mice and improving their learning and memory behavior.
Objective: To observe the improve effects of traditional Chinese medicine( TCM) Yixiao Decoction on learning,memory behavior of the KKAy mice. Methods: A total of 24 male KKAy mice were randomly divided into model group and TCM group.A total of 12 male C57 BL/6 J mice were normal group. The mice of different groups were treated with relevant medicinal respectively by intragastrical method,as the TCM group were fed with Yixiao Decoction. The model group and normal group were fed with distilled water. All groups of mice were performed Morris water maze behavior test respectively when the finish of gavage for 4 and 8 weeks. The Morris water maze's tracking and analytic system of the experiential animal trace was adopted to record the escape latency,the swimming trajectory of the space exploration test,times across the platform and initial angle,etc. Results: Compared with the normal group,the escape latency of the model group was obviously increased at 4 and 8 weeks after intragastric administration( P < 0. 05 =. And the Chinese medicine group was shorter than that of the model group on the 4 th and 5 th day after 8 weeks of gastric administration( P < 0. 05 =. In the space exploration test,the percentage of time in target quadrant and the crossing times of model group decreased after 8 weeks in contrast with 4 weeks. The difference between the model group and normal group,traditional Chinese medicine group was increased and the initial angle was significantly increased compared with normal group( P < 0. 05 =.The results showed that Yixiao Decoction could shorten the escape latency of diabetic mice,increase the number of times of traversing the original platform and swimming time in the target quadrant. Conclusion: Yixiao Decoction has the effects of delaying the occurrence of cognitive impairment in diabetic mice and improving their learning and memory behavior.
引文
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[10]田文杨,龙泓竹,吴淑馨,等.自发性糖尿病模型KKAy小鼠海马的形态学改变及中药益气养阴散结复方对其影响[J].长春中医药大学学报,2016,32(6):1129-1132.
[11]Morris RGM.Spatial localization does not require the presence of local cues[J].Learn Motiv,1981,12(2):239-260.
[12]王宪玲,贾建平.自发性2型糖尿病小鼠发病早期认知功能的研究[J].首都医科大学学报,2007,28(1):75-77.
[13]张静.补肾醒脑法对2型糖尿病认知功能障碍小鼠性激素信号通路相关激素水平、海马Erα和ErβmRNA的影响研究[D].成都:成都中医药大学,2016.
[14]Yin H,Wang W,Yu W,et al.Changes in Synaptic Plasticity and Glutamate Receptors in Type 2 Diabetic KK-Ay Mice[J].J Alzheimers Dis,2017,57(4):1207-1220.
[15]吕仁和,肖永华,刘滔波.分期论治糖尿病[J].药品评价,2008,5(1):35-37.
[2]潘政雯,刘安诺,刘鸿雁,等.2型糖尿病患者工作记忆损伤特点、影响因素研究进展[J].山东医药,2018,58(27):105-108.
[3]Alagiakrishnan K,Sankaralingam S,Ghosh M,et al.Antidiabetic drugs and their potential role in treating mild cognitive impairment and Alzheimer's disease[J].Discov Med,2013,16(90):277-286.
[4]Strachan MW,Reynolds RM,Marioni RE,et al.Cognitive function,dementia and type 2 diabetes mellitus in the elderly[J].Nat Rev Endocrinol,2011,7(2):108-114.
[5]王淑洁,王双双.中西医结合治疗对老年糖尿病患者糖代谢、血脂及血流动力学的影响[J].新乡医学院学报,2018,35(7):626-628.
[6]Zhai Y,Meng X,Luo Y,et al.Notoginsenoside R1 ameliorates diabetic encephalopathy by activating the Nrf2 pathway and inhibiting NL-RP3 inflammasome activation[J].Oncotarget,2018,9(10):9344-9363.
[7]Huang YC,Tsay HJ,Lu MK,et al.Astragalus membranaceus-Polysaccharides Ameliorates Obesity,Hepatic Steatosis,Neuroinflammation and Cognition Impairment without Affecting Amyloid Deposition in Metabolically Stressed APPswe/PS1dE9 Mice[J].Int J Mol Sci,2017,18(12):475-583.
[8]谭剑斌,赵敏,杨杏芬,等.夏枯草对氧化应激损伤的保护作用研究[J].中国实验方剂学杂志,2016,22(4):89-94.
[9]Chu X,Zhou S,Sun R,et al.Chrysophanol Relieves Cognition Deficits and Neuronal Loss Through Inhibition of Inflammationin Diabetic Mice[J].Neurochem Res.2018 Apr;43(4):972-983.
[10]田文杨,龙泓竹,吴淑馨,等.自发性糖尿病模型KKAy小鼠海马的形态学改变及中药益气养阴散结复方对其影响[J].长春中医药大学学报,2016,32(6):1129-1132.
[11]Morris RGM.Spatial localization does not require the presence of local cues[J].Learn Motiv,1981,12(2):239-260.
[12]王宪玲,贾建平.自发性2型糖尿病小鼠发病早期认知功能的研究[J].首都医科大学学报,2007,28(1):75-77.
[13]张静.补肾醒脑法对2型糖尿病认知功能障碍小鼠性激素信号通路相关激素水平、海马Erα和ErβmRNA的影响研究[D].成都:成都中医药大学,2016.
[14]Yin H,Wang W,Yu W,et al.Changes in Synaptic Plasticity and Glutamate Receptors in Type 2 Diabetic KK-Ay Mice[J].J Alzheimers Dis,2017,57(4):1207-1220.
[15]吕仁和,肖永华,刘滔波.分期论治糖尿病[J].药品评价,2008,5(1):35-37.