摘要
【目的】明确猪源抗菌肽对禽源沙门氏菌和大肠杆菌的抑菌作用,为新型抗菌药物的研发和猪源抗菌肽的生产应用提供科学依据。【方法】以猪源抗菌肽为材料,采用液体培养基抑制测定法,结合体内外试验,分别测定猪源抗菌肽对禽源沙门氏菌和禽源大肠杆菌的抗菌活力值,研究其体内外抑菌作用。【结果】对不同浓度的抗菌肽抗菌活力值的测定结果表明,与0.5、0.2和0.1mg·mL~(-1)的猪源抗菌肽相比较,1.0mg·mL~(-1)的抗菌肽对含有106 CFU·mL~(-1)的菌液抑菌效果最好;与阿莫西林、红霉素、四环素等15种抗生素相比较,猪源抗菌肽对禽源沙门氏菌FJS13株和禽源大肠杆菌FJE61株的抑菌圈最大,其抗菌效果最好;抗菌肽在雏鸡体内的抑菌试验表明,攻毒鸡皮下注射猪源抗菌肽有利于提高雏鸡的存活率,在雏鸡体内也具有一定的抑菌作用。【结论】猪源抗菌肽可用于抑制禽源沙门氏菌和大肠杆菌。
【Objective】Activity of porcine antibacterial peptides(PAP)against avian Salmonellaand Escherichia coli was determined for drug development.【Method】Inhibitive activity of PAP on avian pathogens was studied in vitro on an liquid medium and by an in vivo test.【Results】PAP at a concentration of 1.0 mg·mL~(-1) showed a higher bacteriostatic effect on the solution contaning 10~6 CFU·mL~(-1) of the pathogens than at 0.5,0.2 or 0.1 mg·mL~(-1).Compared with 15 antibiotics,such as amoxicillin,erythromycin,tetracycline,etc.,PAP induced the largest inhibition zone on the growth of avian Salmonella FJS13 and E.coli FJE61.In a challenging test on chicken,the PAP administration improved the survival rate of the subcutaneous injected birds.【Conclusion】PAP could be used as a bioagent to combat the infection on chickens by avian Salmonellaand E.coli.
引文
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