真空臭氧消毒机对水果杀菌效果的实验研究
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摘要
目的观察真空臭氧消毒机对水果的杀菌效果。方法以大肠杆菌(Escherichia coli) 8099作为指示微生物,以苹果、圣女果、草莓作为受试对象,采用模拟现场试验进行研究。结果在消毒机箱内压强为50 k Pa的条件下,对苹果分别消毒30 s、60 s、90 s,其平均杀灭对数值依次为4. 52±0. 36、5. 17±0. 06、6. 27±0. 23,杀灭率均高达100%;对圣女果分别消毒150 s、180 s、210 s,其平均杀灭对数值依次为2. 23±0. 11、2. 30±0. 09、3. 21±0. 09,平均杀灭率分别为(99. 40±0. 14)%、(99. 49±0. 10)%、(99. 94±0. 01)%;对草莓分别消毒360 s、480 s、600 s,其平均杀灭对数值依次为1. 52±0. 13、1. 71±0. 35、2. 22±0. 57,平均杀灭率分别为(96. 90±0. 85)%、(97. 69±1. 36)%、(99. 01±1. 03)%。结论在本实验条件下,真空臭氧消毒机对水果表面的大肠杆菌有一定的杀菌效果,且作用时间较短,可以用于杀灭水果表面的微生物。
Objective To observe the sterilization effect of vacuum ozone disinfector on fruit. Methods Using Escherichia coli( Escherichia coli) 8099 as the indicator microorganism,the experiment was carried out with simulated field experiments with apples,cherry tomatoes and strawberries as subjects. Results Under the condition that the pressure was 50 kPa in the disinfection machine box,the average killing value of apple disinfected 30 s,60 s and 90 s is 4. 52 ± 0. 36,5. 17 ± 0. 06,6. 27± 0. 23,and the killing rate was as high as 100%. The average killing rate of cherry tomatoes disinfection of 150 s,180 s and210 s was 2. 23 ± 0. 11,2. 30 ± 0. 09,3. 21 ± 0. 09,and the average killing rate was( 99. 40 ± 0. 14) %,( 99. 49 ± 0. 10)%,( 99. 94 ± 0. 01) %. The average killing value was 1. 52 ± 0. 13,1. 71 ± 0. 35,2. 22 ± 0. 57,and the average killing rate was( 96. 90 ± 0. 85) %,( 97. 69 ± 1. 36) %,( 99. 01 ± 1. 03) %. Conclusion The vacuum ozone disinfector has a certain bactericidal effect on the Escherichia coli on the fruit surface,and the effect of time is shorter,which can be used to kill microbes on the surface of the fruit.
Objective To observe the sterilization effect of vacuum ozone disinfector on fruit. Methods Using Escherichia coli( Escherichia coli) 8099 as the indicator microorganism,the experiment was carried out with simulated field experiments with apples,cherry tomatoes and strawberries as subjects. Results Under the condition that the pressure was 50 kPa in the disinfection machine box,the average killing value of apple disinfected 30 s,60 s and 90 s is 4. 52 ± 0. 36,5. 17 ± 0. 06,6. 27± 0. 23,and the killing rate was as high as 100%. The average killing rate of cherry tomatoes disinfection of 150 s,180 s and210 s was 2. 23 ± 0. 11,2. 30 ± 0. 09,3. 21 ± 0. 09,and the average killing rate was( 99. 40 ± 0. 14) %,( 99. 49 ± 0. 10)%,( 99. 94 ± 0. 01) %. The average killing value was 1. 52 ± 0. 13,1. 71 ± 0. 35,2. 22 ± 0. 57,and the average killing rate was( 96. 90 ± 0. 85) %,( 97. 69 ± 1. 36) %,( 99. 01 ± 1. 03) %. Conclusion The vacuum ozone disinfector has a certain bactericidal effect on the Escherichia coli on the fruit surface,and the effect of time is shorter,which can be used to kill microbes on the surface of the fruit.
引文
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[2]鄢元波,燕南,丁昆仑,等.臭氧消毒对村镇饮用水生物稳定性的影响[J].中国农村水利水电,2016(3):100-103,106.
[3]徐阳,傅燕翔,王慧娟.医用臭氧消毒柜测试方法[J].计量与测试技术,2016,43(12):21-22.
[4]陈贵秋,庄世锋,朱应凯,等.电解式臭氧空气净化消毒机消毒性能的研究[J].中国消毒学杂志,2008,25(5):494-496.
[5]中华人民共和国卫生部.消毒技术规范(2002年版)[M].北京:中华人民共和国卫生部,2002:162.
[6]张秀文,张永寿,郑庆海,等.便携式野战预真空臭氧消毒灭菌器的研制及其效果检测[J].实用医药杂志,2012,29(6):554-555.
[7]许玉姣.环境空气中臭氧浓度变化影响因素的相关性分析[J].甘肃科技纵横,2017,46(9):41-43,68.
[8]崔树玉,李子尧,孙启华,等.臭氧对空气消毒影响因素的研究[J].中华医院感染学杂志,2008,18(10):1392-1394.
[9] Turnbull GA,Morgan JA,Whipps JM,et al. The role of motility in the in vitro attachment of Pseudomonas putida Pa W8 to wheat Roots[J]. FEMS Microbiology Ecology,2001,35(1):57-65.