真空臭氧消毒机对微生物杀灭效果的实验研究
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摘要
目的观察一种新型真空臭氧消毒机的杀菌效果。方法以大肠杆菌8 099、金黄色葡萄球菌6 538、鼠伤寒沙门菌为受试菌种,采用载体定量杀菌试验和模拟现场试验进行研究,按照《消毒卫生规范》(2002)版进行评价。结果:在臭氧浓度为8. 33 mg/L、消毒机箱内压强为50 k Pa的条件下,该消毒机对玻片上的大肠杆菌、金黄色葡萄球菌、鼠伤寒沙门菌消毒60 s后,3种菌的平均杀灭对数值分别为6. 04±0. 02、5. 90±0. 18、5. 91±0. 19;对苹果表面的大肠杆菌、金黄色葡萄球菌、鼠伤寒沙门菌消毒90 s后,3种菌的平均杀灭对数值分别为5. 13±0. 02、6. 32±0. 20、5. 22±0. 08。结论真空臭氧消毒机对微生物具有良好的杀灭效果。
Objective To observe the sterilization effect of a new vacuum ozone sterilizer. Methods Taking Escherichia coli8099,Staphylococcus aureus 6538 and Salmonella typhus of mice as the test strains,quantitative germicidal test and simulated field test of vectors were used to evaluate in accordance with disinfection hygiene standards( 2002). Results Under the condition of an ozone concentration of 8. 33 mg/L and pressure of 50 k Pa,after 60 seconds of disinfection of Escherichia coli,Staphylococcus aureus and Salmonella typhoid on the glass slides,the average killing pairs of the disinfectant were 6. 04 ±0. 02,5. 90 ± 0. 18 and 5. 91 ± 0. 19,respectively. After 90 seconds sterilization of Escherichia coli,Staphylococcus aureus and Salmonella typhoid on the apple surface,the average killing pairs were 5. 13 ± 0. 02,6. 32 ± 0. 20 and 5. 22 ± 0. 08,respectively. Conclusion The vacuum ozone sterilizer has a good sterilization effect on the microorganism.
Objective To observe the sterilization effect of a new vacuum ozone sterilizer. Methods Taking Escherichia coli8099,Staphylococcus aureus 6538 and Salmonella typhus of mice as the test strains,quantitative germicidal test and simulated field test of vectors were used to evaluate in accordance with disinfection hygiene standards( 2002). Results Under the condition of an ozone concentration of 8. 33 mg/L and pressure of 50 k Pa,after 60 seconds of disinfection of Escherichia coli,Staphylococcus aureus and Salmonella typhoid on the glass slides,the average killing pairs of the disinfectant were 6. 04 ±0. 02,5. 90 ± 0. 18 and 5. 91 ± 0. 19,respectively. After 90 seconds sterilization of Escherichia coli,Staphylococcus aureus and Salmonella typhoid on the apple surface,the average killing pairs were 5. 13 ± 0. 02,6. 32 ± 0. 20 and 5. 22 ± 0. 08,respectively. Conclusion The vacuum ozone sterilizer has a good sterilization effect on the microorganism.
引文
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[9] Xu WY,Xu XB,Lin MY,et al. Long-term trends of surface ozone and its influencing factors at the Mt Waliguan GAW station,ChinaPart 2:The roles of anthropogenic emissions and climate variability[J]. Atmospheric Chemistry&Physics,2018,18(2):773-798.
[2] Mao YQ,Guo D,Yao WK,et al. Effects of conventional ozonation and electro-peroxone pretreatment of surface water on disinfection by-product formation during subsequent chlorination[J]. Water Research,2018,130:322-332.
[3]中华人民共和国卫生部.卫生部关于印发《消毒技术规范》(2002年版)的通知[EB/OL].[2018-10-28]. http://www. moh. gov. cn/zhjcj/s9139/200804/86d017920ad84e64a90806717719624f. shtml? tdsourcetag=s_pcqq_aiomsg.
[4] Lim KT,Teh CS, Thong KL. Loop-mediated isothermal amplification assay for the rapid detection of Staphylococcus aureus[J]. BioMed Research International,2013:895816.
[5]曲梅,黄瑛,吕冰,等.北京市肠道门诊腹泻儿童沙门菌感染状况和耐药性分析[J].现代预防医学,2017,44(22):4091-4095.
[6]许玉姣.环境空气中臭氧浓度变化影响因素的相关性分析[J].甘肃科技纵横,2017,46(9):41-43,68.
[7] Sychantha D,Little DJ,Chapman RN,et al. Pat B1 is an Oacetyltransferase that decorates secondary cell wall polysaccharides[J]. Nature Chemical Biology,2018,14(1):79-85.
[8]秦彦珉,饶健,叶宝英,等.臭氧水消毒器对微生物杀灭效果的试验观察[J].现代预防医学,2006,33(9):1615-1616.
[9] Xu WY,Xu XB,Lin MY,et al. Long-term trends of surface ozone and its influencing factors at the Mt Waliguan GAW station,ChinaPart 2:The roles of anthropogenic emissions and climate variability[J]. Atmospheric Chemistry&Physics,2018,18(2):773-798.