通窍活血汤对颅脑损伤模型大鼠海马CA1区超微结构及神经元修复与突触重塑影响
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摘要
目的:分析通窍活血汤对颅脑损伤(TBI)模型大鼠海马CA1区超微结构及神经元修复与突触重塑影响。方法:选取由安康市人民医院实验动物中心提供SPF级Wistar雄性大鼠30只,随机随机数字表法将大鼠分成三组,模型组(10只)、正常组(10只)和观察组(10只),模型组、观察组制备TBI模型,造模第二天起观察组大鼠采用通窍活血汤10g/kg/d灌胃,模型组和正常组大鼠给予生理盐水灌胃,共进行四周。给药后检测大鼠神经功能缺损(m NSS)状况,对大鼠行水迷宫检测,详细记录大鼠正在2分钟内目标象限运动距离、目标区域进入次数及逃避潜伏期状况,SABC法检测大鼠海马CA1区域内SYN1和BDNF蛋白表达状况,观察大鼠海马CA1区电镜超微结构。结果:模型组和观察组大鼠给药1、2周,模型组给药3、4周其m NSS评分较正常组显著升高,观察在升高幅度低于模型组,差异均有统计学意义(P<0. 05);模型组大鼠逃避潜伏期较正常组显著升高,观察组大鼠逃避潜伏期较模型组显著降低,差异均有统计学意义(P<0. 05);观察组大鼠突触素1 (SYN1)、脑源性神经生长因子(BDNF)蛋白表达量较正常组、模型组显著升高,差异有统计学意义(P<0. 05)。结论:通窍活血汤可提升TBI大鼠海马区内SYN1、BDNF表达量,改善大鼠神功功能与认知功能。
Objective: To analyze the effect of Tongqiao Huoxue Decoction on ultrastructure of hippocampal CA1 region,neuronal repair and synaptic remodeling in craniocerebral injury( TBI) model rats. Methods: Thirty male Wistar rats with SPF were provided by the Experimental Animal Center of our hospital. The rats were divided into three groups by randomized random number table. The model group( 10 rats),the normal group( 10 rats) and the observation group( 10 rats) TBI models were prepared in the model group and the observation group,and the rats in the observation group were treated with Tongqiao Huoxue Decoction( 10 g/kg/d) on the second day after model establishment. The rats in the model group and the normal group were given intragastric administration for a total of four weeks. Rats were tested for neurological deficit( m NSS) after drug administration. The rats were examined by water maze. The rats were recorded in detail about the target quadrant movement distance,number of target area entries,and escape latency during 2 minutes. The rat hippocampus was detected by SABC method. The expression of SYN1 and BDNF protein in CA1 region was observed. The ultrastructure of rat hippocampal CA1 region was observed. Results: In the model group and the observation group rats for 1 and 2 weeks,the m NSS scores in the model group were significantly higher than those in the normal group at the 3 rd and 4 th week after administration,and the difference was statistically significant in the observation group( P < 0. 05). The escape latency of the model group was significantly higher than that of the normal group. The escape latency of the observation group was significantly lower than that of the model group( P < 0. 05). The rats in the observation group were synaptophysin. The expression of 1( SYN1) and brain-derived nerve growth factor( BDNF) protein was significantly higher than that in the normal group and the model group,and the difference was statistically significant( P < 0. 05). Conclusion: Tongqiao Huoxue Decoction can increase the expression of SYN1 and BDNF in the hippocampus of TBI rats,and improve the divine function and cognitive function of rats.
Objective: To analyze the effect of Tongqiao Huoxue Decoction on ultrastructure of hippocampal CA1 region,neuronal repair and synaptic remodeling in craniocerebral injury( TBI) model rats. Methods: Thirty male Wistar rats with SPF were provided by the Experimental Animal Center of our hospital. The rats were divided into three groups by randomized random number table. The model group( 10 rats),the normal group( 10 rats) and the observation group( 10 rats) TBI models were prepared in the model group and the observation group,and the rats in the observation group were treated with Tongqiao Huoxue Decoction( 10 g/kg/d) on the second day after model establishment. The rats in the model group and the normal group were given intragastric administration for a total of four weeks. Rats were tested for neurological deficit( m NSS) after drug administration. The rats were examined by water maze. The rats were recorded in detail about the target quadrant movement distance,number of target area entries,and escape latency during 2 minutes. The rat hippocampus was detected by SABC method. The expression of SYN1 and BDNF protein in CA1 region was observed. The ultrastructure of rat hippocampal CA1 region was observed. Results: In the model group and the observation group rats for 1 and 2 weeks,the m NSS scores in the model group were significantly higher than those in the normal group at the 3 rd and 4 th week after administration,and the difference was statistically significant in the observation group( P < 0. 05). The escape latency of the model group was significantly higher than that of the normal group. The escape latency of the observation group was significantly lower than that of the model group( P < 0. 05). The rats in the observation group were synaptophysin. The expression of 1( SYN1) and brain-derived nerve growth factor( BDNF) protein was significantly higher than that in the normal group and the model group,and the difference was statistically significant( P < 0. 05). Conclusion: Tongqiao Huoxue Decoction can increase the expression of SYN1 and BDNF in the hippocampus of TBI rats,and improve the divine function and cognitive function of rats.
引文
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[15]赵敏,刘湘衡,王伟民,等.文拉法辛治疗轻型颅脑损伤后认知功能障碍的临床研究[J].中国微侵袭神经外科杂志,2016,21(11):496~499
[16]李琴,李志强.早期应用高压氧联合调神疏肝针电刺激法治疗颅脑损伤后认知功能障碍疗效观察和安全性评价[J].辽宁中医药大学学报,2016,9(12):124~127
[2]张玉琴,马君芳,孟艳艳.中度创伤性脑损伤患者血清神经元特异性烯醇化酶、S100-β蛋白变化及其与认知功能障碍的相关性[J].中华创伤杂志,2017,33(10):27~31
[3] Li PY,Li C F,Li S. Effect of hyperbaric oxygen therapy on BI index,GCS,SAS and SDS in patients with craniocerebral injury[J].Journal of Hainan Medical University,2017,23(16):210~214
[4]焦明义,赵明光.颅脑损伤术后颅骨缺损与脑认知功能损害的研究进展[J].中华神经外科杂志,2016,32(1):97~99
[5]沈夏锋,吴军发,于惠贤,等.一种适用于康复研究的局灶性颅脑外伤大鼠模型[J].中国康复,2014,5(3):163~166
[6] Chen J,Li Y,Zhang R,et al. Combination therapy of stroke in rats with a nitric oxide donor and human bone marrow stromal cells enhances angiogenesis and neurogenesis[J]. Brain Research,2004,15(2):21~28
[7]贾强,只达石,黄慧玲,等.促红细胞生成素对创伤性脑损伤大鼠认知功能影响的实验研究[J].中华神经医学杂志,2010,09(7):686~689
[8] Guo F Y,Liu T,Chen J J,et al. Changes in phagocytosis and expression of microglial cells in craniocerebral injury mice models[J]. J Biol Regul Homeost Agents,2016,30(2):517~521
[9]徐召溪,徐国政.爆炸冲击波致轻型颅脑损伤患者血脑屏障损伤机制及其与迟发性神经功能障碍的关系[J].解放军医学杂志,2016,41(5):425~429
[10] Saichan X,Wei C,Qinglong F,et al. Plasma cortisol as a noninvasive biomarker to assess severity and prognosis of patients with craniocerebral injury[J]. Eur Rev Med Pharmacol Sci,2016,20(18):3835~3838
[11]张国梁,周承扬,庞坚,等.颅脑损伤后认知功能障碍的机制及其药物治疗进展[J].浙江中医药大学学报,2017,41(5):441~446
[12] Jiang W W,Wang Q H,Liao Y J,et al. Effects of dexmedetomidine on TNF-αand interleukin-2in serum of rats with severe craniocerebral injury.[J]. Bmc Anesthesiology,2017,17(1):130~134
[13]张博,杨明飞.颅脑损伤脑功能成像的研究进展[J].实用医学杂志,2016,32(19):3136~3138
[14] Brawanski N,Baumgarten P,Konczalla J,et al. Cerebral foreign body granuloma in brain triggering generalized seizures without obvious craniocerebral injury:A case report and review of the literature[J]. Surgical Neurology International,2016,7(29):775~778
[15]赵敏,刘湘衡,王伟民,等.文拉法辛治疗轻型颅脑损伤后认知功能障碍的临床研究[J].中国微侵袭神经外科杂志,2016,21(11):496~499
[16]李琴,李志强.早期应用高压氧联合调神疏肝针电刺激法治疗颅脑损伤后认知功能障碍疗效观察和安全性评价[J].辽宁中医药大学学报,2016,9(12):124~127