抗疲劳功能饮料配方用量及其功效研究
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摘要
通过小鼠试验探讨不同用量组合的配方功能饮料抗疲劳的作用。方法:160只雄性ICR小鼠按体重随机分为4组,即溶剂对照组、配方饮料组1、配方饮料组2和阳性对照组。以经口灌胃方式分别给予各组蒸馏水、两个不同剂量受试样品以及某西洋参保健口服液,每日1次,连续30d。而后各组进行负重游泳试验以及血清尿素、肝糖原和血乳酸含量的测定。结果:与溶剂对照组相比,两个剂量受试样品组均能够显著增加小鼠负重游泳的时间(P<0.01)、显著降低小鼠游泳后体内血清尿素的含量水平(P<0.01)、显著降低血乳酸曲线下面积(P<0.01),配方饮料组1还能显著提高小鼠肝糖原含量水平(P<0.01)。结论:该配方功能饮料具有缓解小鼠体力疲劳的功能,其作用效果与牛磺酸和咖啡因的含量水平相关。
The objective was to research the anti-fatigue effects of formula functional beverage with different dosages through the mouse test. Methods: According to the body weight,160 male ICR mice were randomly divided into 4 groups(solvent control group,formula beverage group 1,formula beverage group 2 and the positive control group). Each had oral oral gavage of distilled water,two different doses of samples and certain American ginseng health oral liquid respectively with once a day for 30 consecutive days. Then,serum urea,liver glycogen and levels of lactate acid were determined before and after swimming tests. Results: Compared with solvent control group,two sample groups could significantly increase the swimming time(P<0.01),significantly reduce the level of serum urea in the body after swimming(P<0.01),significantly lower the area under the blood lactic acid curve(P<0.01),and formula beverage sample group 1 could also significantly increase the content of liver glycogen(P<0.01). Conclusion: The formula functional beverage has anti-fatigue effects in mice and its effect is related to the content of taurine and caffeine.
The objective was to research the anti-fatigue effects of formula functional beverage with different dosages through the mouse test. Methods: According to the body weight,160 male ICR mice were randomly divided into 4 groups(solvent control group,formula beverage group 1,formula beverage group 2 and the positive control group). Each had oral oral gavage of distilled water,two different doses of samples and certain American ginseng health oral liquid respectively with once a day for 30 consecutive days. Then,serum urea,liver glycogen and levels of lactate acid were determined before and after swimming tests. Results: Compared with solvent control group,two sample groups could significantly increase the swimming time(P<0.01),significantly reduce the level of serum urea in the body after swimming(P<0.01),significantly lower the area under the blood lactic acid curve(P<0.01),and formula beverage sample group 1 could also significantly increase the content of liver glycogen(P<0.01). Conclusion: The formula functional beverage has anti-fatigue effects in mice and its effect is related to the content of taurine and caffeine.
引文
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[2]宋福杰.浅议运动疲劳的自由基损伤[J].内江科技,2010(7):40.
[3]赵瑞芹,冯敬业.国内外亚健康问题的研究进展及对比分析[J].国外医学情报,2002,23(3):14-17.
[4]杨俊丽,崔杨.疲劳亚健康状态人群中医体质特征分析[J].中国中医药科技,2018,25(1):122-123.
[5]程兆宇,房磊.蓝靛果钙果抗疲劳饮料的研制[J].食品研究与开发,2015,36(20):63-66.
[6]郭敬廷,郭享,赵梦蝶,等.黑参黑蒜抗疲劳饮料的研制[J].吉林医药学院学报,2018,39(2):91-94.
[7]张容标,林云,陈冠敏,等.复方牛磺酸肌醇口服液缓解小鼠体力疲劳的研究[J].预防医学论坛,2014,20(12):920-922.
[8]PEACOCK A,MARTIN F H,CARR A.Energy drink ingredients.Contribution of caffeine and taurine to performance outcomes[J].Appetite,2013,64:1-4.
[9]FUKUDA D H,SMITH A E,KENDALL K L,et al.The possible combinatory effects of acute consumption of caffeine,creatine and amino acids on the improvement of anaerobic running performance in humans[J].Nutrition Research,2010,30(9):607-614.
[10]李海霞,陈榕,周丹,等.咖啡因的合成及其药理作用的研究进展[J].华西药学杂志,2011,26(2):182-187.
[11]罗敏荣,熊正英.赖氨酸与运动能力的关系[J].四川体育科学,2006(3):43-46.
[12]汪多仁.肌醇的开发与应用进展[J].化工中间体,2002,(18/19):23-25.
[13]CHEN Yinbin,WANG Yufang,HOU Wei,et al.Effect of B-complex vitamins on the antifatigue activity and bioavailability of ginsenoside Re after oral administration[J].Journal of Ginseng Research,2017,41(2):119-121.
[14]RICHARDSON J H.The effect of vitamin B6 on muscle fatigue[J].Sports Med Phys Fitness,1981,21(2):209-214.
[15]白宣英.生脉宝口服液有恢复疲劳作用[J].国外医学中医中药分册,2001,23(3):190.
[16]翟鹏贵,刘小杰,舒志成,等.牛磺酸复合饮料抗疲劳的实验研究[J]饮料工业,2013,26(1):16-18.
[17]方文龙.太阳神“圣力”功能饮料的生理功效[J].广州食品工业科技,1995(4):34-38.
[18]卫生部.保健食品检验与评价技术规范[S].北京,2003:87-93.
[19]杨小英,刘华钢,陈刚毅,等.牛磺酸抗运动性疲劳的研究及进展[J]体育科技,2006,27(4):46-50.
[20]NARKHEDE A N,JAGTAP S D,NIRMAL P S,et al.Anti-fatigue effect of Amarkand on endurance exercise capacity in rats[J].BMC Complementary and Alternative Medicine,2016,16(23):1-7.
[21]肖立宁,陈剑伟,高朝辉,等.氨基酸维生素制剂的抗疲劳作用研究[J].解放军医学杂志,2012,37(1):23-25.
[22]许文豪.运动生物化学概论[M].北京:高等教育出版社,2001:47-56.