姜黄素对小鼠急性酒精肝损伤的保护作用
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摘要
目的:研究姜黄素减轻酒精导致急性肝损伤的保护功能。方法:60只小鼠被随机均分为5组,分别为空白对照组、乙醇模型组、治疗低剂量组、中剂量组、高剂量组。每日经口灌胃给予受试样品,空白对照组和模型对照组给予蒸馏水。给予受试样品结束时将乙醇模型组及各治疗组一次灌胃给予50%乙醇,空白对照组给蒸馏水。检测小鼠肝脏匀浆液的丙二醛(MDA)、谷胱甘肽(GSH)、甘油三酯(TG)以及血清中丙氨酸氨基转移酶(ALT)和天门冬酸氨基转移酶(AST)。结果:与模型组比较,各治疗剂量组肝匀浆液中MDA和TG水平(MDA:17.237±2.214,12.511±3.791,17.377±4.641;TG:0.446±0.075,0.512±0.132,0.479±0.136)(P<0.05)均显著降低,GSH显著升高(GSH:40.918±8.324,20.787±12.606,21.377±12.041)(P<0.01);血清AST及ALT水平(AST:173.650±12.607,110.879±10.945,127.399±8.212;ALT:65.988±13.588,45.957±9.304,55.745±15.802)均显著降低(P<0.01)。结论:姜黄素能够减轻酒精诱导的急性肝损伤。
Aim: To study the protective effect of curcumin to alleviate acute liver injury caused by alcohol. Method: 60 mice were randomly divided into 5 groups: blank control group,ethanol model group,the curcumin treatment low dose group,middle dose group and high dose group(40,80,120 mg/kg BW). Mice in the treatment groups were intragastrically infused with different doses of curcumin daily,and the blank control group and model control group were given distilled water daily. At the end of the test,the ethanol model group and each treatment group were given 50% ethanol at one time and the distilled water was given to the blank control group. The levels of malondialdehyde(MDA),glutathione(GSH),triglyceride(TG)in the liver homogenate of mice were estimated. The levels of serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST)were measured. Results: Compared with the ethanol model group,from low dose treatment to high dose treatment,the levels of MDA and TG in the liver homogenate were significantly decreased(MDA: 17.237±2.214,12.511±3.791,17.377±4.641;TG: 0.446±0.075,0.512±0.132,0.479±0.136)(P<0.05),and the levels of GSH was significantly increased(GSH: 40.918±8.324,20.787±12.606,21.377±12.041)(P <0.01). The levels of serum AST and ALT were significantly decreased(AST: 173.650±12.607,110.879±10.945,127.399 ±8.212;ALT: 65.988 ±13.588,45.957±9.304,55.745±15.802)(P<0.01). Conclusion: Curcumin can mitigate the alcohol-induced acute hepatic injury in vivo.
Aim: To study the protective effect of curcumin to alleviate acute liver injury caused by alcohol. Method: 60 mice were randomly divided into 5 groups: blank control group,ethanol model group,the curcumin treatment low dose group,middle dose group and high dose group(40,80,120 mg/kg BW). Mice in the treatment groups were intragastrically infused with different doses of curcumin daily,and the blank control group and model control group were given distilled water daily. At the end of the test,the ethanol model group and each treatment group were given 50% ethanol at one time and the distilled water was given to the blank control group. The levels of malondialdehyde(MDA),glutathione(GSH),triglyceride(TG)in the liver homogenate of mice were estimated. The levels of serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST)were measured. Results: Compared with the ethanol model group,from low dose treatment to high dose treatment,the levels of MDA and TG in the liver homogenate were significantly decreased(MDA: 17.237±2.214,12.511±3.791,17.377±4.641;TG: 0.446±0.075,0.512±0.132,0.479±0.136)(P<0.05),and the levels of GSH was significantly increased(GSH: 40.918±8.324,20.787±12.606,21.377±12.041)(P <0.01). The levels of serum AST and ALT were significantly decreased(AST: 173.650±12.607,110.879±10.945,127.399 ±8.212;ALT: 65.988 ±13.588,45.957±9.304,55.745±15.802)(P<0.01). Conclusion: Curcumin can mitigate the alcohol-induced acute hepatic injury in vivo.
引文
[1]World Health Organization.2018 Global Status Report on Alcohol and Health.
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[8]Bao W,Li K,Rong S,et al.Curcumin alleviates ethanol-induced hepatocytes oxidative damage involving heme oxygenase-1 induction[J].J Ethnopharmacol,2010,128(2):549-553.
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[2]中华医学会肝病学分会脂肪肝和酒精性肝病学组.酒精性肝病诊疗指南(2010年修订版)[J].中华肝脏病杂志,2010,18(3):167-170.
[3]汪正辉,王泰龄,等.酒精性肝病[M].北京:中国医药科技出版社,2000:3-50,61-69,116-122,128-135.
[4]舒志成,徐容容.天然化合物姜黄素的生物学作用综述[J].饮料工业,2017,20(4):55-58.
[5]王政,张静,惠伯棣,等.姜黄素对小鼠肝损伤的影响[J].食品科学,2007,28(10):501-503.
[6]Chang Guo,Jingfan Ma,Qionghong Zhong.Curcumin improves alcoholic fatty liver by inhibiting fatty acid biosynthesis[J].Toxicology and Applied Pharmacology,2017,328:1-9.
[7]Hae-In Lee,Robin A.Mc Gregor,Myung-Sook Choi.Low doses of curcumin protect alcohol-induced liver damage by modulation of the alcohol metabolic pathway,CYP2E1 and AMPK[J].Life Sciences,2013,93:693-699.
[8]Bao W,Li K,Rong S,et al.Curcumin alleviates ethanol-induced hepatocytes oxidative damage involving heme oxygenase-1 induction[J].J Ethnopharmacol,2010,128(2):549-553.
[9]肖潇,刘烈刚.姜黄素对慢性酒精肝损伤保护作用的研究[D].武汉:华中科技大学,2012.