岩陀黄酮化合物毒理性试验
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摘要
为研究岩陀黄酮化合物的毒理性,通过对小鼠的急性经口试验和35d亚慢性毒理试验的方式进行安全计量范围的测定。饲喂小鼠的岩陀黄酮化合物含量为67.462%,小鼠急性经口试验无死亡情况,最大耐受量为3 000mg/kg体质量。连续灌胃小鼠35d后,低、中、高剂量组小鼠体质量、内脏系数和生化指标与对照组相比无显著性差异,组织切片无明显的病变损伤。结果表明,岩陀黄酮化合物在本试验计量范围内属于安全无毒。
In order to study the toxicological properties of the flavonoid fromRodgersia Sambucifolia Hemsl,the safe dose measurement range was determined by the acute oral experiment and the35-day sub-chronic toxicological test in mice.The rhizoma rodgersiae flavonoid compound purity was 67.462%,and there was no death in mice acute oral experiment,the maximum tolerated dose is 3 000mg/kg of body weight.After 35 days of continuous gavage,the body weight,visceral coefficient and biochemical indexes of mice in low dose group,medium dose group and high dose group had no significant difference compared with the control group,and there was no obvious lesion damage in the tissue slices.The experiment indicated that the dose scope of the rhizoma rodgersiae flavonoid which was used in the test was safe and non-toxic.
In order to study the toxicological properties of the flavonoid fromRodgersia Sambucifolia Hemsl,the safe dose measurement range was determined by the acute oral experiment and the35-day sub-chronic toxicological test in mice.The rhizoma rodgersiae flavonoid compound purity was 67.462%,and there was no death in mice acute oral experiment,the maximum tolerated dose is 3 000mg/kg of body weight.After 35 days of continuous gavage,the body weight,visceral coefficient and biochemical indexes of mice in low dose group,medium dose group and high dose group had no significant difference compared with the control group,and there was no obvious lesion damage in the tissue slices.The experiment indicated that the dose scope of the rhizoma rodgersiae flavonoid which was used in the test was safe and non-toxic.
引文
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[2]延玺,刘会青,邹永青,等.黄酮类化合物生理活性及合成研究进展[J].有机化学,2008,28(9):1534-1544.
[3]KUMAR S,PANDEY A K.Chemistry and biological activities of flavonoids:a overview[J].Sci World J,2013:162750.
[4]王凯,王洋,孙娟娟,等.苜蓿和红车轴草黄酮提取物对绵羊生长性能和血液相关指标的影响[J].中国兽医学报,2017,37(4):704-709.
[5]朴向民,于营,SIN H H,等.桔梗的体外抗氧化活性及总多酚和黄酮苷元含量分析[J].吉林农业大学学报,2017,39(5):579-584
[6]占今舜,王冰,胡金杰,等.大豆黄酮作为饲料添加剂的应用前景[J].粮食与饲料工业,2013,7:44-46.
[7]谭玲玲,王忠,胡正海.桔梗中总黄酮的提取工艺优化[J].西北药学杂志,2014,11(6):568-570.
[8]张鹏,赵强,杨莉,等.超声波提取加拿大蓬总黄酮的研究[J].中国野生植物资源,2011,30(2):36-37.
[9]王珊珊,吴春.AB-8大孔树脂对芫荽黄酮的纯化工艺研究[J].哈尔滨商业大学学报(自然科学版),2014,30(1):33-35.
[10]邢桂珍,崔玉梅,胡汐柔,等.甘草总黄酮混悬液的急性毒性与长期毒性试验[J].黑龙江畜牧兽医,2014(21):155-156.
[11]钟正贤,周桂芬,陈学芬,等.广西藤茶总黄酮的长期毒性试验[J].时珍国医国药,2003,14(4):193-195.