不同PRRS弱毒苗免疫血清对FcγRIIb介导ADE效应的影响
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摘要
为了研究蓝耳病不同毒株疫苗在ADE效应下的表现,将疫苗血清复合物(用105.0TCID50的HP-PRRSV田间分离株ZJ-2014,与不同弱毒疫苗CH-1R、R98、TJM-F92的免疫血清在不同的稀释梯度下混合作用24 h,获得血清复合物)接种于PAM细胞,进行蓝耳病病毒增殖试验,检测病毒RNA拷贝数。以兔抗猪FcγRIIb血清进行封闭构建对照组,而后接种PAM细胞。通过比较封闭前后PRRS病毒增殖数量的差异,确定不同疫苗血清兔抗猪FcγRIIb多抗对ADE的阻断作用。试验结果表明:TJM-F92株疫苗的血清抗原复合物对ADE效应的阻断效果最为明显,提示在未来蓝耳病疫苗研制及临床应用中,TJMF92株可以成为优选毒株。
In order to study ADE effect of differrent stains of PRRSV,The HP-PRRSV field isolate ZJ-2014 with 105.0 TCID50 was mixed with the immune serum of three vaccines(TJM-F92,CH-1 R,R98) for 24 hours under different dilution gradients. Antigen-antibody complexes of different neutralized states were inoculated with PAM for PRRSV proliferation assay, and PRRSV copy number was detected by PCR. The control group used rabbit anti-porcine FcγR before inoculation of PAM with antigen-antibody complex.IIb serum to block the FcγR on the cell surfaceIIb. Comparing the same vaccine sera in the test group and the control group at the same dilution, the difference in the amount of virus was harvested to determine the rabbit anti-porcine FcγRIIb multi-antibody blockade of ADE. The results showed that TJM-F92 strain attenuated vaccine immune serum can significantly weaken FcγRIIb mediated ADE effect.Its suggested that TJM-F92 can be the preferred stanin in the development.
In order to study ADE effect of differrent stains of PRRSV,The HP-PRRSV field isolate ZJ-2014 with 105.0 TCID50 was mixed with the immune serum of three vaccines(TJM-F92,CH-1 R,R98) for 24 hours under different dilution gradients. Antigen-antibody complexes of different neutralized states were inoculated with PAM for PRRSV proliferation assay, and PRRSV copy number was detected by PCR. The control group used rabbit anti-porcine FcγR before inoculation of PAM with antigen-antibody complex.IIb serum to block the FcγR on the cell surfaceIIb. Comparing the same vaccine sera in the test group and the control group at the same dilution, the difference in the amount of virus was harvested to determine the rabbit anti-porcine FcγRIIb multi-antibody blockade of ADE. The results showed that TJM-F92 strain attenuated vaccine immune serum can significantly weaken FcγRIIb mediated ADE effect.Its suggested that TJM-F92 can be the preferred stanin in the development.
引文
[1]谢印乾,沈志强,王桂花,等.猪繁殖与呼吸综合征免疫学研究进展[J].动物医学进展,2006,27(6):6-10.
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[5]袁宝,任文陟.猪繁殖与呼吸综合征病毒分子生物学研究进展[J].吉林畜牧兽医,2006,27(1):35-37.
[6]蒋志政,张改平,刘明阳,等.抗猪FcγRⅡb多抗阻断猪繁殖与呼吸综合征病毒抗体依赖增强作用研究[J].河南农业科学,2012,41(4):139-142.
[7]杨青原,秦运杰,张宜娜,等.不同特性IgG促进PRRSV在PAM细胞中的增值作用[J].中国兽医学报,2014,36(6):859-865.
[8]段二珍,周永辉.FcγRIIb介导的PRRS感染的抗体依赖性增强作用[J].中国兽医学报,2012,32(8):1105-1109.
[9]史平玲,王爱萍,乔松林,等.IgG Fc家畜受体研究进展[J].河南农业科学,2010(1):124-128.
[10]冯丽丽,张丽萍,张改平,等.脂多糖对猪肺泡巨噬细胞分泌IL-1β及TNF-α的影响[J].河南农业科学,2009(3):106-109.
[2]冯俊杰,罗雅艳,周俊梅,等.登革病毒2型prM蛋白的表达及其抗体的ADE作用研究[J].热带医学杂志,2013(4):377-381,411.
[3]Yoon K J,Wu L L,Zimmerman J J,et al.Antibody-dependent enhancement(ADE)of porcine reproductive and respiratory syndrome virus(PRRSV)infection in pigs[J].Viral Immunol,1996,9(1):51-63.
[4]仇华吉,童光志.抗体依赖性增强作用的免疫生物学特点及其意义[J].中国兽医科技,1999,29(7):19-20.
[5]袁宝,任文陟.猪繁殖与呼吸综合征病毒分子生物学研究进展[J].吉林畜牧兽医,2006,27(1):35-37.
[6]蒋志政,张改平,刘明阳,等.抗猪FcγRⅡb多抗阻断猪繁殖与呼吸综合征病毒抗体依赖增强作用研究[J].河南农业科学,2012,41(4):139-142.
[7]杨青原,秦运杰,张宜娜,等.不同特性IgG促进PRRSV在PAM细胞中的增值作用[J].中国兽医学报,2014,36(6):859-865.
[8]段二珍,周永辉.FcγRIIb介导的PRRS感染的抗体依赖性增强作用[J].中国兽医学报,2012,32(8):1105-1109.
[9]史平玲,王爱萍,乔松林,等.IgG Fc家畜受体研究进展[J].河南农业科学,2010(1):124-128.
[10]冯丽丽,张丽萍,张改平,等.脂多糖对猪肺泡巨噬细胞分泌IL-1β及TNF-α的影响[J].河南农业科学,2009(3):106-109.