板蓝根色胺酮金属配合物合成及与DNA切割作用研究
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摘要
通过溶剂热法首次合成来源板蓝根等菘蓝植物的两个色胺酮铁配合物[Fe(Try)_2]CH_3OH(1),[Fe(I-Try)_4](2),并得到晶体结构。通过琼脂凝胶电泳法,紫外可见光谱法研究表明,色胺酮铁配合物对DNA具有较强切割能力,且配合物2强于配合物1,结合常数为4.29×10~4dm~3·mol~(-1)和5.21×10~5dm~3·mol~(-1)。
Two complexes [Fe( Try)_2]CH_3OH( 1),[Fe( I-Try)_4]( 2) of tryptanthrin from medicinal plants of Isatis indigotica were synthesized by solvothermal method for the first time,and the crystal structure was obtained. The results showed that the cleavage ability of DNA for the two complexes was improved by agarose gel electrophoresis and UV spectroscopy,and the complex2 was stronger than that of complex 1,the binding constant was 4. 29 × 10~4 dm~3 mol~(-1) and 5. 21 × 10~5 dm~3 mol~(-1).
Two complexes [Fe( Try)_2]CH_3OH( 1),[Fe( I-Try)_4]( 2) of tryptanthrin from medicinal plants of Isatis indigotica were synthesized by solvothermal method for the first time,and the crystal structure was obtained. The results showed that the cleavage ability of DNA for the two complexes was improved by agarose gel electrophoresis and UV spectroscopy,and the complex2 was stronger than that of complex 1,the binding constant was 4. 29 × 10~4 dm~3 mol~(-1) and 5. 21 × 10~5 dm~3 mol~(-1).
引文
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[2]缪珊,孙纪元,谢艳华,等.色胺酮的研究进展[J].中国药理学通报,2008,24(2):152.
[3]Chen ZF,Liu YC,Liang H,et al.Potential new inorganic antitumour agents from combining the anticancer traditional Chinese medicine(TCM)liriodenine with metal ions,and DNA binding studies[J].Dalton Trans,2009,(2):262.
[4]Chen ZF,Liang H.Progresses in TCM metal-based antitumour agents[J].Anticancer Agents Med Chem,2010,10:412.
[5]陈振锋,彭艳,谭明雄,等.基于中药活性成分的金属基抗肿瘤药物前期研究[J].化学进展,2009,21(5):929.
[6]李力,徐永莉,张月云,等.DNA分子标记在中药鉴定中的应用及发展趋势分析[J],时珍国医国药,2009,20(11):2845.
[7]Hu FL,Wang HF,Guo D,et al.Controlledformation of chiral networks and their reversible chiroptical switchingbehaviour by UV/microwave irradiation[J].Chem.Commun.,2016,52:7990.
[8]Hu FL,Wu W,Liang P,et al.Construction ofentangled coordination polymers based on M2+ions,4,4'-{[1,2-phenylenebis(methylene)]bis(oxy)}dibenzoateand different n-donor ligands[J].Cryst Growth Des,2013,13:5050.
[9]Hu FL,Wang SL,Lang JP,et al.In-situ X-ray diffraction snapshotting:determination of the kinetics of a photodimerization within asingle crystal[J].Sci Rep,2014,4:6815.
[10]Harsh RH,Barton JK,Novel dipyridophenazine complexes of ruthenium(II):exploring luminescent reporters of DNA[J].J.Am.Chem.Soc.1992,114:5919.
[11]Tong CL,Hu Z,Liu WP,Study on the interaction between paraquat and calf thymus DNA by spectral methods[J].Acta Chim Sin,2010,68:1404.