芜菁离体培养再生体系建立
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摘要
【目的】研究不同激素对芜菁再生不定芽,不定根的影响;并建立芜菁再生体系。【方法】设置2种浓度NAA激素0. 1 mg/L,0. 5 mg/L;和4种浓度6-BA激素0. 25、0. 5、0. 75、1. 0 mg/L处理2种新疆芜菁、1种温州盘菜的子叶、下胚轴。观察记录子叶、下胚轴的不定芽再生频率和再生根频率。【结果】三种芜菁下胚轴产生不定芽的频率为:柯坪芜菁(75. 32%)>温州盘菜(73. 73)%>喀什芜菁(72. 55%)。NAA为0. 5 mg/L时下胚轴的生根率要高于NAA为0. 1 mg/L时的生根率。【结论】三种芜菁的下胚轴诱导不定芽的效果要优于子叶的诱导效果;柯坪芜菁与喀什芜菁不定芽诱导最适激素浓度为6-BA 0. 75 mg/L、NAA 0. 1 mg/L;温州盘菜最适激素浓度为6-BA 0. 5 mg/L、NAA 0. 1 mg/L。
【Objective】To explore the influence of different hormones on the adventitious buds and roots of turnips( Brassica rapa L.) and establish turnips regeneration system.【Method】Two concentrations of NAA0. 1 mg/L and 0. 5 mg/L and 4 concentrations of 6-BA hormone 0. 25,0. 5,0. 75,1. 0 mg/L were set up to treat the cotyledons and hypocotyls of two kinds of Xinjiang turnips,one kind of Wenzhou for observing the frequency of adventitious bud regeneration and regenerated roots. 【Result】The regeneration rate of three hypocotyls was: Keping( 75. 32%) > Wenzhou( 73. 73%) > Kashi( 72. 55%). When NAA was at0. 5 mg/L,the hypocotyls rooting rate was higher than that when NAA was at 0. 1 mg/L.【Conclusion】The effect of Hypocotyl inducing adventitious bud of three kinds of turnip was better than that cotyledons. The optimal hormone concentrations of adventitious bud induction turnips in Keping and Kashi are 6-BA 0. 75 mg/L and NAA 0. 1 mg/L. and the optimal hormone concentration of Wenzhou dish is 6-BA 0. 5 mg/L and NAA0. 1 mg/L.
【Objective】To explore the influence of different hormones on the adventitious buds and roots of turnips( Brassica rapa L.) and establish turnips regeneration system.【Method】Two concentrations of NAA0. 1 mg/L and 0. 5 mg/L and 4 concentrations of 6-BA hormone 0. 25,0. 5,0. 75,1. 0 mg/L were set up to treat the cotyledons and hypocotyls of two kinds of Xinjiang turnips,one kind of Wenzhou for observing the frequency of adventitious bud regeneration and regenerated roots. 【Result】The regeneration rate of three hypocotyls was: Keping( 75. 32%) > Wenzhou( 73. 73%) > Kashi( 72. 55%). When NAA was at0. 5 mg/L,the hypocotyls rooting rate was higher than that when NAA was at 0. 1 mg/L.【Conclusion】The effect of Hypocotyl inducing adventitious bud of three kinds of turnip was better than that cotyledons. The optimal hormone concentrations of adventitious bud induction turnips in Keping and Kashi are 6-BA 0. 75 mg/L and NAA 0. 1 mg/L. and the optimal hormone concentration of Wenzhou dish is 6-BA 0. 5 mg/L and NAA0. 1 mg/L.
引文
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[2]马和木提,米丽班·霍加,拜合提努尔. GC-MS法测定芜菁子中油脂组成研究[J].新疆师范大学学报(自然科学版),2012,31(2):49-51.Mahamuti,Miliban·Huojia,Baihetinure.(2012). Determination of oil compositions in Brassica rapa L. seed by Gas Chromatography-Mass[J]. Journal of Xinjiang Normal University(Natural Sciences Edition),31(2):49-51.(in Chinese)
[3]孙莲,张丽静,符继红,等. GC-MS分析新疆芜菁子中挥发油的化学成分[J].华西药学杂志,2007,22(4):374-375.SUN Lian,ZHANG Li-jing,FU Ji-hong,et al.(2007).Chemical components of the essential oil from the seeds of Brassica rapa L. by GC-MS[J]. West China Journal of Pharmaceutical Sciences,22(4):374-375.(in Chinese)
[4]周芳,海力茜·陶尔大洪,郁文.维药恰麻古儿化学成分预实验[J].海峡药学,2009,21(6):100-102.ZHOU Fang,Hailiqian Taoerdahong,YU Wen.(2009). Preliminary experiment on chemical composition of uygur medicine[J]. Strait Pharmaceutical Journal,21(6):100-102.(in Chinese)
[5]拜年.维药恰麻古儿化学成分的基础研究[D].乌鲁木齐:新疆医科大学硕士学位论文,2011.BAI Nian.(2011). Fundamental studies on chemical constituents of Brassica rapa L.[D]. Master Dissertation. Xinjiang Medical University,Urumqi.(in Chinese)
[6]刘晔峰,龚凌霄,刘连亮,等.西藏芜菁营养成分测定及提高缺氧耐受性的动物实验研究[J].食品工业科技,2012,33(9):412-416.LIU Ye-feng,GONG Lin-xiao,LIU Lian-liang,et al.(2012). Determination on nutritional content of Tibetan turnip(Brassica rapa L.)and experimental study on improvement of mice hypoxia tolerance[J]. Science and Technology of Food Industry,33(9):412-416.(in Chinese)
[7]陶月良,邱君正,林华,等.芜菁、萝卜和大头菜块根品质及营养价值比较研究[J].特产研究,2002,24(1):37-40.TAO Yue-liang,QIU Jun-zheng,LIN Hua,et al.(2002).Comparative studies on quality and nutrient value of the tubers of three Brassica vegetables among Turnip,Radish and kohlrahi[J]. Special Wild Economic Animal and Plant Research,24(1):37-40.(in Chinese)
[8]马菁.新疆恰玛古中类黄酮提取、纯化及微胶囊化的研究[D].石河子:石河子大学硕士学位论文,2010.MA Jing.(2010). Study on extraction,purification and the microcapsule of Xingjiang Qiamagu[D]. Master Dissertation. Shihezi University,Shihezi.(in Chinese)
[9]马彦玲.芜菁子的化学成分基础研究[D].乌鲁木齐:新疆医科大学硕士学位论文,2011.MA Yan-ling.(2011). Fundamental studies on the chemical constituents of Brassica rapa L. seed[D]. Master Dissertation.Xinjiang Medical University,Urumqi.(in Chinese)
[10]王菁.新疆芜菁总黄酮止咳、平喘、祛痰药理作用的研究[D].乌鲁木齐:新疆医科大学硕士学位论文,2013.WANG Jing,(2013). Study on Xinjiang tuinip total falconoid of relieving cough and asthma,expectorant effect[D]. Master Dissertation. Xinjiang Medical University,Urumqi.(in Chinese)
[11]陈卓尔,古娜娜·对山别克,乌英,等.新疆芜菁水提物抗肿瘤活性初步研究[J].西北药学杂志,2016,(3):264-267.CHEN Zhuo-er,Gunana Duishanbieke,WU Ying,et al.(2016). Study on the anti-tumor effect of aqueous extract from Brassica rapa L.[J]. Northwest Pharmaceutical Journal,(3):264-267.(in Chinese)
[12]马龙,屈卫东,邓淑文,等.新疆芜菁抗衰老作用的实验研究[J].新疆医学院学报,1998,(1):36-39.MA Long,QU Wei-dong,DENG Shu-wen,et al.(1998).Experimental research on the antiaging effects of Xinjiang Turnip[J]. Journal of Xinjiang Medical University,(1):36-39.(in Chinese)
[13]王伟,杨晓君,高蕾,等.响应面法优化新疆芜菁多糖提取工艺及体外抗氧化活性研究[J].食品工业科技,2018,39(2):229-233.WANG Wei,YANG Xiao-jun,GAO Lei,et al.(2018). Optimization the extraction process of polysaccharide by response surface methodology from Brassica rapa L. and its antioxidant activity[J]. Science and Technology of Food Industry,39(2):229-233.(in Chinese)
[14]张谦筱,安熙强,白利平,等.恰玛古多糖的抗氧化功能及其片剂的制备工艺[J].食品安全质量检测学报,2017,8(6):2079-2 085.ZHANG Qian-xiao,AN Xi-qiang,BAI Li-ping,et al.(2017). Antioxidant function of Brassica rapa L. polysaccharide and processing technology of its tablet[J]. Journal of Food Safety&Quality,8(6):2,079-2,085.(in Chinese)
[15]张昌伟.芜菁花叶病毒诱导的基因沉默系统在不结球白菜各器官的沉默效应[C].中国园艺学会.中国园艺学会2017年论文摘要集.中国园艺学会:2017:1.ZHANG Chang-wei.(2017). The silencing effect of the gene silencing system induced by turnip Mosaic virus in different organs of Chinese cabbage[C]. Proceedings of the Annual Meeting of China Horticultural Society:1(in Chinese)
[16]祝富祥.芜菁花叶病毒的分子变异及其P3蛋白在病毒与寄主互作中的功能研究[D].长春:吉林大学硕士学位论文,2016.ZHU Fu-xiang.(2016). Molecular variability of Turnip mosaic virus and the function of P3 protein in the interaction between virus and host[D]. Master Dissertation. Jilin University,Changchun.(in Chinese)
[17]王艳.芜菁花叶病毒编码蛋白与拟南芥At SWEET1蛋白互作研究[D].长春:吉林大学硕士学位论文,2017.WANG Yan.(2017). The interaction between turnip mosaic virus encoded proteins and At SWEET1 protein in Arabidopsis thaliana[D]. Master Dissertation. JILin University,Changchun.(in Chinese)
[18]王艳.芜菁花叶病毒编码蛋白与拟南芥SWEET家族蛋白的互作研究[C].中国植物病理学会.中国植物病理学会2016年学术年会论文集.中国植物病理学会:2016:1.WANG Yan.(2016). Study on the interaction between the encoding protein of turnip Mosaic virus and the SWEET family protein of arabidopsis thaliana[C]. Proceedings of the Annual Meeting of Chinese Society for Plant Pathology:1(in Chinese)
[19]赵学敏,夏宏勋.芜菁花叶病毒研究进展[J].现代农业科技,2008,(17):170-171,173.ZHAO Xue-min,XIA Hong-xun.(2008). Research progress of turnip Mosaic virus[J]. Modern Agricultural Science and Technology,(17):170-171,173.(in Chinese)
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