芝麻饼肥中芝麻素和芝麻林素含量的测定
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摘要
利用高效液相色谱法同时测定芝麻饼肥中芝麻素和芝麻林素的含量。液相色谱条件为:流动相为甲醇水溶液(V_(甲醇)∶V_水=80∶20),流速1.0 m L/min,进样量5.0μL,柱温为室温,紫外检测器波长287 nm。检验结果表明:被测两峰完全分离,且峰形较好,芝麻素和芝麻林素均在1~100 mg/L范围内线性关系良好,R~2分别为1.000 0、0.999 9;该方法的精密度、重现性及准确度均较好,芝麻素平均回收率在98.2%~100.2%之间,芝麻林素平均回收率在96.7%~99.8%之间;样品提取液在2 h内稳定。芝麻饼肥中芝麻素和芝麻林素总含量为1.2~133.7 mg/100 g,芝麻素与芝麻林素含量比例为0.9~3.1。试验验证了高效液相色谱法同时测定芝麻饼肥中芝麻素、芝麻林素含量的可行性,为芝麻饼肥的质量检测提供了技术支持。
The contents of sesamin and sesamolin in sesame cake fertilizer samples were determined by high-performance liquid chromatography at the same time. The determination conditions of high-performance liquid chromatography were as follows: methanol solution(V_(methanol)∶V_(water)=80 ∶20) as mobile phase,flow rate 1.0m L/min,injection volume 5.0 μL, column temperature room temperature, and UVD wavelength 287 nm.Results showed that the two tested peaks were separated completely and had good peak shapes; both the sesamin and sesamolin had good linear relationship within 1 to 100 μg/m L with correlation coefficients of 1.000 0 and0.999 9, respectively. The method had high precision, high repeatability and high accuracy; the average recoveries of sesamin and sesamolin were within 98.2% to 100.2% and 96.7% to 99.8%,respectively; and the sample extract was stable in two hours. The total content of sesamin and sesamolin in sesame cake fertilizer was1.2 to 133.7 mg/100 g,and the ratio of sesamin to sesamolin was 0.9 to 3.1. The experiments verified the feasibility of using high-performance liquid chromatography to determine sesamin and sesamolin in sesame cake fertilizer at the same time,thereby providing technical support for quality detection of sesame cake fertilizer.
The contents of sesamin and sesamolin in sesame cake fertilizer samples were determined by high-performance liquid chromatography at the same time. The determination conditions of high-performance liquid chromatography were as follows: methanol solution(V_(methanol)∶V_(water)=80 ∶20) as mobile phase,flow rate 1.0m L/min,injection volume 5.0 μL, column temperature room temperature, and UVD wavelength 287 nm.Results showed that the two tested peaks were separated completely and had good peak shapes; both the sesamin and sesamolin had good linear relationship within 1 to 100 μg/m L with correlation coefficients of 1.000 0 and0.999 9, respectively. The method had high precision, high repeatability and high accuracy; the average recoveries of sesamin and sesamolin were within 98.2% to 100.2% and 96.7% to 99.8%,respectively; and the sample extract was stable in two hours. The total content of sesamin and sesamolin in sesame cake fertilizer was1.2 to 133.7 mg/100 g,and the ratio of sesamin to sesamolin was 0.9 to 3.1. The experiments verified the feasibility of using high-performance liquid chromatography to determine sesamin and sesamolin in sesame cake fertilizer at the same time,thereby providing technical support for quality detection of sesame cake fertilizer.
引文
[1]SUJA K P,ABRAHAM J T,THAMIZH S N,et al.Antioxidant efficacy of sesame cake extract in vegetable oil protection[J].Food Chemistry,2004,84:393-400.
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[3]武雪萍,刘增俊,赵跃华,等.施用芝麻饼肥对植烟根际土壤酶活性和微生物碳、氮的影响[J].植物营养与肥料学报,2005,11(4):541-546.
[4]李泳雪,曾伟杰,吴斌.薄层扫描法测定便通胶囊中芝麻素的含量[J].中国中药杂志,2002,27(7):517-518.
[5]冯志勇,谷克仁.紫外光谱法测定芝麻素与芝麻林素含量[J].中国粮油学报,2006,21(3):296-300.
[6]陈伟,刘红彦,倪云霞.C18净化-HPLC测定芝麻中芝麻素和芝麻林素含量[J].中国油脂,2011,36(3):71-74.
[2]SUJA K P,JAYALEKSHMY A,ARUMUGHANC.Antioxidant activity of sesame cake extract[J].Food Chemistry,2005,91:213-219.
[3]武雪萍,刘增俊,赵跃华,等.施用芝麻饼肥对植烟根际土壤酶活性和微生物碳、氮的影响[J].植物营养与肥料学报,2005,11(4):541-546.
[4]李泳雪,曾伟杰,吴斌.薄层扫描法测定便通胶囊中芝麻素的含量[J].中国中药杂志,2002,27(7):517-518.
[5]冯志勇,谷克仁.紫外光谱法测定芝麻素与芝麻林素含量[J].中国粮油学报,2006,21(3):296-300.
[6]陈伟,刘红彦,倪云霞.C18净化-HPLC测定芝麻中芝麻素和芝麻林素含量[J].中国油脂,2011,36(3):71-74.