摘要
为了解鸡源致病性大肠杆菌的生物学特性和耐药性,试验通过采集病料、细菌分离培养、16S rRNA基因鉴定、致病性试验、药敏试验等方法。结果表明:分离出的20株细菌的培养特征、镜检特征均符合大肠杆菌特征;16S rRNA基因序列经BLAST比对与大肠杆菌同源性达99.9%~100%,确定20株分离菌为鸡源大肠杆菌;20株大肠杆菌的致病力为40%~100%;10株鸡源致病性大肠杆菌对15种抗菌药物均呈现多重耐药,耐药率为33.3%~93.3%,耐药情况较严重和复杂。
To understand the biological traits and drug resistance of pathogenic Escherichia coli from chicken, we conducted the study by collecting disease materials, bacterial isolation and culture, 16 S rRNA gene identification,pathogenicity test, drug sensitivity test and other tests. The results show that the culture and microscopic traits of the20 isolates are consistent with the traits of E. coli; the 16 S rRNA gene sequence was 99.9%-100% homologous to E.coli by BLAST, and 20 isolates were identified as chicken-derived Escherichia coli; 20 strains of Escherichia coli have a pathogenicity of 40% to 100%; 10 strains of chicken-derived pathogenic Escherichia coli have multi-drug resistance to 15 antimicrobial agents, and the resistance rate is 33.3%-93.3%. The drug-resistant situation is serious and complicated.
引文
[1]张俊丰,黄东璋.鸡肠道大肠杆菌的分离鉴定及耐药性分析[J].中国畜牧兽医,2013,40(11):179-183.
[2]于阿芳.鸡致病性大肠杆菌的药敏试验、血清型鉴定及耐药基因的PCR检测[J].泰安:山东农业大学,2009.
[3]王宇,王永芬.鸡大肠杆菌病的流行病学研究[J].信阳师范学院学报,2007,20(3):317-340.
[4] MURRAY B E.New aspects of antimicrobial resistence and resulting the therapeutic dilemmas[J].Infect Dis,1991,163:1185.
[5]严玉霖,雷华.鹅大肠杆菌的分离及药敏试验[M].中国畜牧兽医,2011,38(10):160-162.
[6]秦双双,张红.鸭源致病性大肠杆菌的分离鉴定及致病性观察[J].湖北农业科学,2011,50(1):119-121.
[7]冯方周,张晓冬.辽西地区致病性鸡大肠杆菌的分离鉴定及16SrRNA序列分析[J].黑龙江畜牧兽医,2017(15):191-195.
[8]张轶,王春光.猪源大肠杆菌的分离、鉴定及耐药性分析[J].中国农学通报,2005,21(12):23-30.
[9]刘玉涛,吴学敏.福建省鸡源大肠杆菌的分离鉴定及药敏试验[M].中国农学通报,2014,30(2):65-68.
[10]郝智慧,肖希龙.不同区域鸡大肠杆菌对抗菌药的耐药耐药性比较[J].中国收兽医科学,2009,39(1):84-88.
[11]常宏伟,赵俊.17株产H2S致泄大肠埃希菌的微生物学研究大肠杆菌的分离鉴定及药敏试验[J].中华疾病控制杂志,2009,(3):320-323.
[12]刁有祥,李久芹.山东省鸡大肠杆菌的分离鉴定[J].中国预防兽医学报,2002,24(1):21-23.
[13]徐海花,牛仲相.鸡源致病性大肠杆菌地方株的分离与鉴定[J].山东家禽,2004:7-10.
[14]孙协军,陈琴琴.六安市鸡大肠杆菌的分离鉴定及药敏试验[J].畜牧与饲料科学,2008,5:43-45.
[15]师福山,赵德明.禽大肠杆菌的分离与16SrRNA的鉴定[J].中国畜牧兽医,2009,36(2):111-113.