珍珠提取物对黑色素细胞酪氨酸酶活性和黑色素合成的影响
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摘要
研究珍珠提取物对小鼠黑素瘤细胞内酪氨酸酶活性及黑色素合成的影响,为珍珠提取物在美白领域的应用提供理论依据。实验对珍珠提取物进行了成分分析,并以小鼠皮肤黑色素瘤细胞(B16F10)为细胞模型,采用CCK-8法测定珍珠提取物对黑色素细胞活力的影响,以L-DOPA为底物测定细胞内酪氨酸酶活性,采用比色法测定细胞中的黑色素含量。实验结果:在一定添加量下,珍珠提取物对B16F10细胞没有毒性,且能抑制酪氨酸酶活性,减少黑色素的生成。表明珍珠提取物能有效抑制黑色素合成,达到美白的效果。
In order to study the effect of pearl extract on tyrosinase activity and melanin synthesis in murine melanoma cells and the mouse skin melanoma cells( B16 F10) were used as cell model. In this experiment,the component of pearl extract has been analyzed and CCK-8 method was used to determine the cell proliferation rate,the activity of melanocytes in the cells was determined by L-DOPA method,and the content of melanin in the cells was determined by colorimetry. The results showed that pearl extract had no toxicity to B16 F10 cells,and have effects to inhibit tyrosinase activity and reduce melanin production. Experiments showed that pearl extract can effectively inhibit melanin synthesis to achieve the effect of whitening.
In order to study the effect of pearl extract on tyrosinase activity and melanin synthesis in murine melanoma cells and the mouse skin melanoma cells( B16 F10) were used as cell model. In this experiment,the component of pearl extract has been analyzed and CCK-8 method was used to determine the cell proliferation rate,the activity of melanocytes in the cells was determined by L-DOPA method,and the content of melanin in the cells was determined by colorimetry. The results showed that pearl extract had no toxicity to B16 F10 cells,and have effects to inhibit tyrosinase activity and reduce melanin production. Experiments showed that pearl extract can effectively inhibit melanin synthesis to achieve the effect of whitening.
引文
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[2]杨安全,王菁,张丽华,等.珍珠提取物的美白功效研究[J].药物生物技术,2016,23(02):146-149.
[3]杨安全,沈玥琦,张丽华,等.珍珠活性成分的护肤功效研究[J].香料香精化妆品,2016,(01):58-61.
[4]努尔买买提·艾买提,哈木拉提·吾甫尔,吐尔逊·吾甫尔.异常黏液质成熟剂对B16F10黑色素瘤细胞的增殖、酪氨酸酶活性、黑色素的合成的影响[J].中成药,2007,29(12):1824-1826.
[5]张蓉.RES对添加有b FGF的小鼠B16F10黑素瘤细胞的细胞增殖及黑素生成的影响[D].湖南中医药大学,2013.
[6]张玮,高静,张敏,等.7种美白化合物对皮肤黑素细胞的体外生物学效应[J].药学实践杂志,2011,29(01):24-26,40.
[7]汪涌,何清濂,林子豪.黑素细胞与影响皮肤色素沉着的因素[J].实用美容整形外科杂志,1997,(03):152-153.
[8]徐学涛,张焜,杜志云,等.天然产物对酪氨酸酶抑制作用的研究进展[J].广东化工,2007,34(11):53-56.
[9]杨壮群,王正辉,荔鹏,等.芦荟苦素对体外培养的黑素细胞影响的实验研究[J].中国美容医学,2003,12(05):464-466+561.
[10]陆晔,朱佩云,项翠琴.3种美白剂对皮肤黑素细胞内酪氨酸酶活性抑制的探讨[J].上海预防医学杂志,2003,15(04):168-170.
[11]刘扬,张占雄.多肽含量的测定方法的比较[J].内蒙古石油化工,2008,34(05):65-65.
[12]鲁伟,任国谱,宋俊梅.蛋白水解液中多肽含量的测定方法[J].食品科学,2005,26(07):169-171.
[13]张玫,王道生,王坚.珍珠燕窝提取液的功效试验[J].药物生物技术,1994,1(02):49-51.
[14]Dai JP,Chen J,Bei YF,et al.Effects of pearl powder extract and its fractions on fibroblast function relevant to wound repair[J].Pharmac Biol,2010:48(2):122-127.
[15]Xu H,Huang K,Gao Q,et al.A study on the prevention and treatment of myopia with nacre on chicks[J].Pharmacological Research,2001,41:1-6.
[16]Shao D Z,Wang C K,Hwang H J,et al.Comparison of hydration,tyrosinase resistance,and antioxidant activation in three kinds of pearl powders[J].Cosm Sci,2010,61:133-145
[17]杨安全,王菁,骆建芬,等.淡水珍珠提取物抗氧化能力的研究[J].药物生物技术,2015,22(03):233-236.
[18]王倩,蔡念宁,金力.中药延缓皮肤衰老的研究现状[J].中国美容医学,2006,15(02):219-221.