摘要
【目的】为了验证观赏海棠叶片McmiR160a对花色素苷代谢的调控作用。【方法】以观赏海棠常色红叶品种‘王族’为试验材料,确定miR160a的前体基因序列并对其靶基因进行预测,克隆McmiR160a前体序列并构建过表达载体并瞬时转入‘王族’组培苗。通过高效液相色谱和qRT-PCR分析瞬时侵染植株中花色素苷的含量及其合成途径中结构基因的表达量。【结果】结果表明,在观赏海棠中过表达McmiR160a可以降低花色素苷的含量,同时降低其靶基因和花色素苷代谢途径关键基因表达水平。【结论】研究结果表明,观赏海棠中McmiR160a对花色素苷的合成具有负调控作用。
【Objective】This experiment was to verify the regulation of miR160a on anthocyanin biosynthesis in Malus spp.leaves.【Methods】We used the leaves of ever-red leaf cultivar ‘Royalty’ of Malus spp.as experimental material.The sequence of miR160a precursor gene and its target genes were predicted.The precursor sequence of McmiR160a was cloned and was transiently over-expressed into the‘Royalty'tissue culture seedlings.And then,the contents of anthocyanin and the expressions of key genes in the pathway of anthocyanin biosynthesis were analyzed by HPLC and qRT-PCR.【Results】The results showed that over-expressing of McmiR160a in Malus spp.leaves decreased the expression of its target gene,the anthocyanin contents,and the expressions of key genes in anthocyanin biosynthesis pathway.【Conclusion】The results suggested that McmiR160a negatively regulated anthocyanins biosynthesis Malus spp..
引文
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