樟叶越桔组培苗生根和移栽技术研究
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摘要
为解决樟叶越桔(Vaccinium dunalianum)组培苗生根质量不佳、移栽成活率低的问题,该研究以樟叶越桔继代苗为材料,采用单因子试验从激素类型及浓度、培养基类型和蔗糖质量浓度对其生根的适宜条件进行筛选,进一步研究了不同基质配比对樟叶越桔移栽苗存活率的影响。结果表明:激素类型和浓度、培养基类型对樟叶越桔生根率的影响最大,其次为蔗糖质量浓度;最适合樟叶越桔生根的激素及浓度为IBA2.0 mg·L~(-1)、基本培养基类型为1/4MS、蔗糖质量浓度为15 g·L~(-1),樟叶越桔组培苗最佳生根培养基为1/4MS+IBA 2.0 mg·L~(-1)+活性炭0.1 g·L~(-1)+蔗糖15 g·L~(-1),生根率达100%,平均生根数为每株7.67条;根系呈辐射状、基部无愈伤组织,组培苗生长健壮、叶色浓绿;樟叶越桔组培苗移栽时以全腐殖土基质为佳,成活率为83.7%,植株叶片舒展,生长状况良好。该研究建立的优化体系有效地提高了樟叶越桔组培生根苗的生根率和生根质量,解决了后期移栽成活困难的问题,为优良的樟叶越桔植株规模化生产提供了科学依据和技术支持。
Vaccinium dunalianum is one of the main plant sources for arbutin which is widely used in cosmetic formulations as the natural active agents to whiten the skin. During the tissue culture process of V. dunalianum,the base of the rooting plantlets obtained by the current culture medium often formed the callus,which significantly affected the survival rate after transplantation. In order to improve the rooting quality of the proliferation plantlets and survival rate of the transplanted plantlets of V. dunalianum. The optimum medium components for the root induction of the proliferation plantlets of V. dunalianum,including hormone types and concentrations,medium types and sucrose concentrations,were analyzed by the single factor experiment,and further probed the effects of different substrate ratios on the survival rate of the transplanted V. dunalianum plantlets in this study. The results showed that the medium types and the hormone types and their concentrations had more significant effects on the rooting rate than sucrose concentration. For the root induction,the optimal hormone and its concentration were IBA 2.0 mg·L~(-1),the optimal media was 1/4 MS and the optimal sucrose concentration was 15 g·L~(-1). The most suitable medium for the root induction of the proliferation plantlets of V. dunalianum was 1/4 MS+ IBA 2.0 mg·L~(-1)+ activated carbon 0.1 mg·L~(-1)+ sucrose 15 g·L~(-1),by which the rooting rate could reach 100%,and the average number of roots was 7.67 per plant. The root system of proliferation plantlets of V. dunalianum was radially extended and none callus was formed at the base of the rooting plantlets. In addition,the tissue culture plantlets of V. dunalianum grew strong and had dark-green leaves. For the transplantation of V. dunalianum plantlets,the optimal media for transplantation was the 100% humus,in which the survival rate reached 83.7%,and the tissue culture plantlets obtained by the experiments grew well and had healthy leaves. The optimization system established by this study,which had effectively improved the roots quality and rooting rate of the proliferation plantlets and the survival rate of the transplanted plantlets of V. dunalianum,and provided scientific and technological support for the largescale production of high quality V. dunalianum plants.
Vaccinium dunalianum is one of the main plant sources for arbutin which is widely used in cosmetic formulations as the natural active agents to whiten the skin. During the tissue culture process of V. dunalianum,the base of the rooting plantlets obtained by the current culture medium often formed the callus,which significantly affected the survival rate after transplantation. In order to improve the rooting quality of the proliferation plantlets and survival rate of the transplanted plantlets of V. dunalianum. The optimum medium components for the root induction of the proliferation plantlets of V. dunalianum,including hormone types and concentrations,medium types and sucrose concentrations,were analyzed by the single factor experiment,and further probed the effects of different substrate ratios on the survival rate of the transplanted V. dunalianum plantlets in this study. The results showed that the medium types and the hormone types and their concentrations had more significant effects on the rooting rate than sucrose concentration. For the root induction,the optimal hormone and its concentration were IBA 2.0 mg·L~(-1),the optimal media was 1/4 MS and the optimal sucrose concentration was 15 g·L~(-1). The most suitable medium for the root induction of the proliferation plantlets of V. dunalianum was 1/4 MS+ IBA 2.0 mg·L~(-1)+ activated carbon 0.1 mg·L~(-1)+ sucrose 15 g·L~(-1),by which the rooting rate could reach 100%,and the average number of roots was 7.67 per plant. The root system of proliferation plantlets of V. dunalianum was radially extended and none callus was formed at the base of the rooting plantlets. In addition,the tissue culture plantlets of V. dunalianum grew strong and had dark-green leaves. For the transplantation of V. dunalianum plantlets,the optimal media for transplantation was the 100% humus,in which the survival rate reached 83.7%,and the tissue culture plantlets obtained by the experiments grew well and had healthy leaves. The optimization system established by this study,which had effectively improved the roots quality and rooting rate of the proliferation plantlets and the survival rate of the transplanted plantlets of V. dunalianum,and provided scientific and technological support for the largescale production of high quality V. dunalianum plants.
引文
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BU CH,ZHANG H,LUO XL,et al.,2018.Establishment of adventitious root culture system of Vaccinium dunalianum[J].JSW For Coll(Nat Sci Ed),38(3):200-205.[卜程洪,张訸,罗旭璐,等,2018.樟叶越桔不定根培养体系建立[J].西南林业大学学报(自然科学版),38(3):200-205.]
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CHEN L,HU SY,2003.Micropropagation of Opuntia dillenii(Ker-Gawl.)Haw.[J].Guihaia,23(3):259-263+290.[程磊,胡宋英,2003.仙人掌的微繁殖[J].广西植物,23(3):259-263+290.]
DE FARIA RT,RODRIGUES FN,DO V.R.OLIVEIAR L,et al.,2004.In vitro Dendrobium nobile plant growth and rooting in different sucrose concentrations[J].Hortic(Bras,Brasilia),22(4):780-783.
DONG CL,2011.A comparative experiment on transplanting survival rate of tissue cultured blueberry plantlets[J].J S Agric Sci,42(11):1375-1377.[董朝莉,2011.蓝莓组培苗移栽存活对比试验[J].南方农业学报,42(11):1375-1377.]
FANG RZ,1986.Studies on Chinese Vaccinium[J].Acta Bot Yunnan,8(3):239-259.[方瑞征,1986.中国越橘属的研究[J].云南植物研究,8(3):239-259.]
FANG XJ,LI YX,WEN GQ,et al.,2014.Study on the tissue culture and rapid propagation of blueberry[J].Seed,33(9):118-120.[房小晶,李永霞,文光琴,等,2014.蓝莓组培与快繁技术研究[J].种子,33(9):118-120.]
FENG HQ,DU BB,WANG QW,et al.,2016.The role of activated carbon in protecting the roots of wheat seedlings under cadmium stress[J].Acta Ecol Sin,36(10):2962-2968.[冯汉青,杜变变,王庆文,等,2016.镉胁迫下活性炭对小麦幼根的保护作用[J].生态学报,36(10):2962-2968.]
FRACRO F,ECHEVERRIGARAY S,2001.Micropagation of Cunila galioides,a popular medicinal plant of South Brazil[J].Plant Cell Tissue Organ Cult,64(1):1-4.
HAN DW,2013.Effect of rooting of tissue culture seedlings of blueberry under IBA[J].Jiangsu Agric Sci,41(7):38-40.[韩德伟,2013.IBA对蓝莓组培苗瓶内生根的影响[J].江苏农业科学,41(7):38-40.]
GONG JY,WANG HX,CHEN BL,et al.,2019.Establishment of tissue culture and rapid propagation system of Dendrobium Enobi Purple‘Splashi’[J].J Southern Agric,50(1):125-130.[龚建英,王华新,陈宝玲,等,2019.泼墨石斛高位芽组培快繁体系的建立[J].南方农业学报,50(1):125-130.]
HE JW,XU ZZ,2007.Tissue culture of Vaccinium duclouxii(Lévl.)Hand.-Mazz[J].Plant Physiol Comm,43(2):320.[和加卫,徐中志,2007.云南越桔的组织培养[J].植物生理学通讯,43(2):320.]
HUANG ZZ,YAN HB,2007.Advance in non-tube rootage technology for test-tube plantlets in vitro[J].Guangxi Agric Sci,38(2):124-12.[黄卓忠,严华兵,2007.试管苗瓶外生根技术[J].广西农业科学,38(2):124-12.]
IKEUCHI M,OGAWA Y,IWASE A,et al.,2016.Plant regeneration:Cellular origins and molecular mechanisms[J].Develop,143(9):1442-1451.
LIANG XJ,DAI GZ,ZHAI XL,2011.Review of progress in tissue culture of blueberry[J].Heilongjiang Agric Sci,(6):138-142.[梁晓晶,代志国,翟熙伦,2011.越橘组织培养研究进展[J].黑龙江农业科学,(6):138-142.]
LIU GL,LIANG ZH,ZHU J,2001.Effects of activated charcoal on plant tissue cultures:A review[J].J Jiangsu For Sci Technol,28(5):46-48.[刘根林,梁珍海,朱军,2001.活性炭在植物组织培养中的作用概述[J].江苏林业科技,28(5):46-48.]
LIU LP,2006.Effect of exogenous sucrose on seed germination,seedling growth,flavonoids accumulation and salt tolerance of roots in commen buckwheat(Fagopyrum esculentum Moench)[D].Nanjing:Nanjing Agricultural University:35-39.[刘丽萍,2006.外源蔗糖对荞麦种子萌发、幼苗生长和黄酮积累及根系耐盐性的影响[D].南京:南京农业大学:35-39.]
LIU ZE,ZHU Y,LOU C,2012.The effects of macronutrient components on tissue culture and rapid propagation of Bambusa multiplex[J].J Bamb Res,31(1):46-51.[刘正娥,朱颜,楼崇,2012.大量元素组成对孝顺竹苗组培快繁的影响[J].竹子研究汇刊,31(1):46-51.]
LUO XL,TANG JR,LI N,et al.,2014a.Tissue culture and rapid propagation of Vaccinium dunalianum[J].Plant Physiol J,50(11):1717-1720.[罗旭璐,唐军荣,李娜,等,2014a.樟叶越桔的组织培养与快速繁殖[J].植物生理学报,50(11):1717-1720.]
LUO XL,ZHANG DG,LI YH,et al.,2014b.Nutritional composition of dried fruits of in Vaccinium dunalianum Wight[J].Jiangsu Agric Sci,42(1):242-244.[罗旭璐,张德国,李永和,等,2014b.樟叶越橘阴干果实的营养成分[J].江苏农业科学,42(1):242-244.]
PRAKASH E,SHA VK,SAIRAM RP,et al.,1999.Regeneration of plants from seed-derived callus of Hybanthus enneaspermus L.Muell.,a rare ethnobotanical herb[J].Plant Cell Rep,18(10):873-878.
RANI G,GROVER IS,1999.In vitro callus induction and regeneration studies in Withania somnifera L.(Dunal)[J].Plant Cell Tiss Org Cult,57(1):23-27.
REN GX,TANG B,OUYANG LP,et al.,2017.Effect of different treatments on the seed germination of Vaccinium dunalianum[J].J Mt Agric Biol,36(4):49-54.[任国香,唐彪,欧阳路平,等,2017.不同处理方法对樟叶越桔种子萌发的影响[J].山地农业生物学报,36(4):49-54.]
TIAN XH,XING YJ,ZHANG JY,et al.,2015.Study on key techniques of in vitro culture from Vaccinnium vitis-idaea L.[J].For Eng,31(2):57-60.[田新华,邢亚娟,张建瑛,等,2015.红豆越橘离体培养关键技术研究[J].森林工程,31(2):57-60.]
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