不同浓度维生素诱导处理对黄瓜幼苗防御酶活性的影响分析
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摘要
为了研究不同维生素处理对黄瓜幼苗防御酶活性的影响,分别用维生素B_1、B_2、B_6和抗坏血酸(VC)对黄瓜幼苗进行诱导处理,以蒸馏水为空白对照,对黄瓜幼苗的防御酶活性进行测定和分析。结果表明:经过维生素诱导处理的黄瓜幼苗,体内的多酚氧化酶(PPO)和过氧化物酶(POD)的活性基本呈现先升高后降低的趋势,而超氧化物歧化酶(SOD)的活性整体呈现升高的趋势,其中经过2.0%维生素B_2诱导处理的黄瓜幼苗体内的SOD活性增强最为显著,可诱导黄瓜植株产生对霜霉病的抗性,增强植株抵抗病虫害的能力。
In order to study the effects of different vitamin treatments on defense enzyme activities of cucumber seedlings, vitamin B_1, B_2, B_6 and ascorbic acid(VC) were used to induce cucumber seedlings. Distilled water was used as blank control. The activities of defensive enzymes in cucumber seedlings were measured and analyzed. The results showed that the activities of polyphenol oxidase(PPO) and peroxidase(POD) in cucumber seedlings treated with vitamin induction increased first and then decreased, while the activities of superoxide dismutase(SOD) increased as a whole. The SOD activity of cucumber seedlings induced by 2.0% vitamin B_2 increased most significantly, which could induce cucumber plants to be resistant to downy mildew and enhance their resistance to pests and diseases.
In order to study the effects of different vitamin treatments on defense enzyme activities of cucumber seedlings, vitamin B_1, B_2, B_6 and ascorbic acid(VC) were used to induce cucumber seedlings. Distilled water was used as blank control. The activities of defensive enzymes in cucumber seedlings were measured and analyzed. The results showed that the activities of polyphenol oxidase(PPO) and peroxidase(POD) in cucumber seedlings treated with vitamin induction increased first and then decreased, while the activities of superoxide dismutase(SOD) increased as a whole. The SOD activity of cucumber seedlings induced by 2.0% vitamin B_2 increased most significantly, which could induce cucumber plants to be resistant to downy mildew and enhance their resistance to pests and diseases.
引文
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[3]严凯,罗泽丽,胡芳丽,等. 6%抗坏血酸水剂对刺梨抗白粉病的诱导效应[J].农药, 2017,(7):528-530.
[4]严凯,伍廷辉,王金利,等. 6%抗坏血酸水剂对猕猴桃抗逆性诱导效果[J].农药, 2018,(3):228-231.
[5]蔡学建,向章敏,李家俊,等. 6%抗坏血酸水剂抗烟草马铃薯Y病毒的作用机制[J].贵州农业科学, 2010, 38(12):106-108.
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[7]张宪政,陈凤玉,王荣富.植物生理学实验技术[M].沈阳:辽宁科学技术出版社, 1994.
[8]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社, 2000.
[9]黑银秀.核黄素诱导番茄抗番茄黄化曲叶病毒病的生理机制[D].南京:南京农业大学, 2011.
[10]张鹏翀,胡增辉,沈应柏.植物诱导抗性的研究进展[J].现代农业科学, 2008,(9):22-23.
[11]佚名.普洱市蔬菜新品种引进试验初报[J].中国果菜,2018, 38(10):39-43.
[12]赵小明.壳寡糖诱导植物抗病性及其诱抗机理的初步研究[D].大连:中国科学院大连化学物理研究所, 2010.