醋栗番茄LA2093渐渗系的构建及花序相关性状QTL分析
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摘要
为了创制一批携带优良农艺性状的番茄种质资源新材料,以栽培番茄品种‘Jina’(Solanum lycopersicum)为受体亲本,以野生醋栗番茄LA2093(S.pimpinellifolium)为供体亲本,利用杂交、3次回交和InDel、SSR分子标记辅助选择方法构建了一套以栽培番茄‘Jina’为遗传背景、含LA2093的基因的渐渗系。从BC_1群体构建的遗传图谱中选择均匀分布于12条染色体的96对InDel、SSR分子标记追踪野生番茄的渗入片段,在BC_3代共留选渐渗系材料51份,其中单片段材料14份、双片段材料27份、三片段材料10份;共得到渗入片段98个,平均单个渗入片段长度为35.69 cM;51份渐渗系平均渗入供体基因片段占受体基因组的比例为5.6%,完全覆盖12条染色体。利用BC_3F_1群体检测到与首花序节位、花序间隔节位、单花序花数3个农艺性状相关的6个QTL位点,单个位点的贡献率为4.33%～47.93%。
In order to create a batch of new tomato germplasm resources with excellent genes, in this study a set of introgression lines were created between the wild tomato LA2093(the donor) and the cultivated tomato "Jina"(the receptor). Through hybridization, three-time backcrossing and InDel and SSR marker-assisted selection, a set of introgression lines have been constructed. They had the genetic background of cultivated tomato "Jina" with the fragment from LA2093. A genetic map was constructed in the BC_1 generation, and 96 pairs of InDel and SSR molecular markers uniformly distributed on the 12 chromosomes were selected to track the infiltrated fragments of wild tomato. Subsequently, 14 single-segment introgression lines, 27 double-segment introgression lines and 10 three-segment introgression lines were obtained in the BC_3 generation. A total of 98 introgression segments were obtained, and the average length of a single introgression segment was 35.69 cM. Each introgression line contained an average of 5.6% of the donor gene. They overlapped and covered 100% of the wild tomato genome. In BC_3F_1 population, 6 QTLs were detected for 3 agronomic traits including the leaf node below the first inflorescence, the node of interval inflorescence and the number of flowers per inflorescence. The contribution rate for a single locus was 4.33%～47.93%.
In order to create a batch of new tomato germplasm resources with excellent genes, in this study a set of introgression lines were created between the wild tomato LA2093(the donor) and the cultivated tomato "Jina"(the receptor). Through hybridization, three-time backcrossing and InDel and SSR marker-assisted selection, a set of introgression lines have been constructed. They had the genetic background of cultivated tomato "Jina" with the fragment from LA2093. A genetic map was constructed in the BC_1 generation, and 96 pairs of InDel and SSR molecular markers uniformly distributed on the 12 chromosomes were selected to track the infiltrated fragments of wild tomato. Subsequently, 14 single-segment introgression lines, 27 double-segment introgression lines and 10 three-segment introgression lines were obtained in the BC_3 generation. A total of 98 introgression segments were obtained, and the average length of a single introgression segment was 35.69 cM. Each introgression line contained an average of 5.6% of the donor gene. They overlapped and covered 100% of the wild tomato genome. In BC_3F_1 population, 6 QTLs were detected for 3 agronomic traits including the leaf node below the first inflorescence, the node of interval inflorescence and the number of flowers per inflorescence. The contribution rate for a single locus was 4.33%～47.93%.
引文
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[15] 邱树亮.潘那利番茄渐渗系的构建及相关农艺性状的定位[D].北京:中国农业科学院,2012.
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[19] Finkers R,Van Heusden A W,Meijer-Dekens F,et al.The construction of a Solanum habrochaites LYC4 introgression line population and the identification of QTLs for resistance to Botrytis cinerea [J].Tag Theoretical & Applied Genetics,2007,114(6):1071.
[20] Barrantes W,Fernández-Del-Carmen A,López-Casado G,et al.Highly efficient genomics-assisted development of a library of introgression lines of Solanum pimpinellifolium [J].Molecular Breeding,2014,34(4):1817-1831.
[21] 陆地棉TM-1背景的尖斑棉染色体片段渐渗系的培育研究[D].南京:南京农业大学,2014.
[22] Arbelaez J D,Moreno L T,Singh N,et al.Development and GBS-genotyping of introgression lines (ILs) using two wild species of rice,O.meridionalis and O.rufipogon,in a common recurrent parent,O.sativa cv.curinga [J].Molecular Breeding,2015,35(2):81.
[23] Tong V G.番茄InDel分子标记的开发与果实心室数量主效QTLlcn2.2的精细定位[D].北京:中国农业科学院,2016.
[24] 潘颖.利用高代回交群体定位醋栗番茄发芽期与幼苗期耐盐QTL及耐盐种质的创制[D].北京:中国农业科学院,2009.
[25] Liu X,Geng X,Zhang H,et al.Association and genetic identification of loci for four fruit traits in tomato using InDel markers [J].Frontiers in Plant Science,2017,8.
[26] Young N D,Tanksley S D.Restriction fragment length polymorphism maps and the concept of graphical genotypes [J].Theoretical & Applied Genetics,1989,77(1):95-101.
[27] Van B R.GGT 2.0:versatile software for visualization and analysis of genetic data [J].Journal of Heredity,2008,99(2):232-236.
[28] Ashrafi H,Kinkade M,Foolad M R.A new genetic linkage map of tomato based on a Solanum lycopersicum × S.pimpinellifolium RIL population displaying locations of candidate pathogen response genes [J].Genome,2009,52(11):935-956.
[29] Sharma A,Zhang L,Niňoliu D,et al.A Solanum lycopersicum × Solanum pimpinellifolium linkage map of tomato displaying genomic locations of R-genes,RGAs,and candidate resistance/defense-response ESTs [J].International Journal of Plant Genomics,2014,2008(4):926090.
[30] Zhou R,Yu X,Ottosen C O,et al.Drought stress had a predominant effect over heat stress on three tomato cultivars subjected to combined stress [J].Bmc Plant Biology,2017,17(1):24.
[31] 李学峰.野生黄瓜代换系的构建[D].北京:中国农业科学院,2010.
[32] 曾瑞珍,Talukdar A,刘芳,等.利用单片段代换系定位水稻粒形QTL[J].中国农业科学,2006,39(4):647-654.
[33] 余文贵,赵统敏,沈新莲,等.野生番茄单片段渐渗系的创建及应用[J].园艺学报,2006,33(1):197-202.
[34] 张桂权,曾瑞珍,丁效华,等.水稻单片段代换系文库的构建及其在分子育种上的应用[C].植物分子育种国际学术研讨会.2005.
[35] Allen K D,Sussex I M.Falsiflora and anantha control early stages of floral meristem development in tomato (Lycopersicon esculentum Mill.) [J].Planta,1996,200(2):254-264.
[36] Molinero-Rosales N,Latorre A,Jamilena M,et al.SINGLE FLOWER TRUSS regulates the transition and maintenance of flowering in tomato [J].Planta,2004,218(3):427-434.
[37] Pnueli L.The SELF-PRUNING gene of tomato regulates vegetative to reproductive switching of sympodial meristems and is the ortholog of CEN and TFL1 [J].Development,1998,125(11):1979-1989.
[38] Lifschitz E,Ayre B G,Eshed Y.Florigen and anti-florigen:a systemic mechanism for coordinating growth and termination in flowering plants [J].Frontiers in Plant Science,2014,5.
[39] 田小琴.番茄花序间隔节位的QTL定位及ShHsfC1的功能分析[D].武汉:华中农业大学,2018.
[40] 位江静,田丽萍,魏亦农,等.加工番茄早熟性状的相关性分析及QTL定位[J].北方园艺,2010(10):8-11.
[41] 李景富,彭艳,许向阳,等.番茄早熟性及QTL定位的研究[J].东北农业大学学报,2015,46(6).
[42] 刘杨,陈火英.利用重组自交系群体进行产量相关性状的QTL定位[C]//中国园艺学会.2008园艺学进展(第八辑):中国园艺学会第八届青年学术讨论会暨现代园艺论坛论文集.中国园艺学会,2008.2.
[43] Tanksley S D,Nelson J C.Advanced backcross QTL analysis:a method for the simultaneous discovery and transfer of valuable QTLs from unadapted germplasm into elite breeding lines [J].Theoretical and Applied Genetics,1996,92(2):191-203.
[2] Tanksley S D,Mccouch S R.Seed banks and molecular maps:unlocking genetic potential from the wild [J].Science,1997,277(5329):1063-1066.
[3] Miller J C,Tanksley S D.RFLP analysis of phylogenetic relationships and genetic variation in the genus Lycopersicon [J].Theoretical & Applied Genetics,1990,80(4):437-448.
[4] Rick C M.CHAPTER 1:the potential of exotic germplasm for tomato improvement [M]//Plant Improvement and Somatic Cell Genetics.Elsevier Inc.1982:1-28.
[5] Alexander L J,Lincoln R E,Wright V.Survey of the genus Lycopersicon for resistance to the important tomato diseases occurring in Ohio and Indiana [J].Plant Disease Reporter,1942(suppl):136.
[6] Tanksley S D,Grandillo S,Fulton T M,et al.Advanced backcross QTL analysis in a cross between an elite processing line of tomato and its wild relative L.pimpinellifolium [J].Tag Theoretical & Applied Genetics,1996,92(2):213-224.
[7] Bernacchi D,Beckbunn T,Emmatty D,et al.Advanced backcross QTL analysis of tomato II:evaluation of near-isogenic lines carrying single-donor introgressions for desirable wild QTL-alleles derived from Lycopersicon hirsutum and L.pimpinellifolium[J].Theoretical & Applied Genetics,1998,97(7):1191-1196.
[8] Chague V,Mercier J C,Guenard M,et al.Identification of RAPD markers linked to a locus involved in quantitative resistance to TYLCV in tomato by bulked segregant analysis [J].Theoretical & Applied Genetics,1997,95(4):671-677.
[9] Chunwongse J,Chunwongse C,Black L,et al.Molecular mapping of the Ph-3 gene for late blight resistance in tomato [J].Journal of Pomology & Horticultural Science,2015,77(3):281-286.
[10] Ashrafi H,Kinkade M P,Merk H L,et al.Identification of novel quantitative trait loci for increased lycopene content and other fruit quality traits in a tomato recombinant inbred line population [J].Molecular Breeding,2012,30(1):549-567.
[11] Monforte A J,Asíns M J,Carbonell E A.Salt tolerance in Lycopersicon species IV:efficiency of marker-assisted selection for salt tolerance improvement [J].Theoretical & Applied Genetics,1996,93(5-6):765-772.
[12] Foolad M R.Breeding for a biotic stress tolerances in tomato [C]//Ashraf M,Harris P J C.Abiotic stresses:plant resistance through breeding and molecular approaches.New York:Haworth,2005:613-684.
[13] 王玉民,席章营,尚爱兰,等.作物单片段代换系的构建及应用[J].中国农学通报,2008,24(3):67-71.
[14] Wehrhahn C,Allard R W.The detection and measurement of the effects of individual genes involved in the inheritance of a quantitative character in wheat [J].Genetics,1965,51(1):109-119.
[15] 邱树亮.潘那利番茄渐渗系的构建及相关农艺性状的定位[D].北京:中国农业科学院,2012.
[16] Eshed Y,Zamir D.A genomic library of Lycopersicon pennellii in L.esculentum:a tool for fine mapping of genes [J].Euphytica,1994,79(3):175-179.
[17] Monforte A J,Tanksley S D.Development of a set of near isogenic and backcross recombinant inbred lines containing most of the Lycopersicon hirsutum genome in a L.esculentum genetic background:a tool for gene mapping and gene discovery [J].Genome,2000,43(5):803.
[18] Canady M A,Meglic V,Chetelat R T.A library of Solanum lycopersicoides introgression lines in cultivated tomato [J].Genome / National Research Council Canada,2005,48(4):685.
[19] Finkers R,Van Heusden A W,Meijer-Dekens F,et al.The construction of a Solanum habrochaites LYC4 introgression line population and the identification of QTLs for resistance to Botrytis cinerea [J].Tag Theoretical & Applied Genetics,2007,114(6):1071.
[20] Barrantes W,Fernández-Del-Carmen A,López-Casado G,et al.Highly efficient genomics-assisted development of a library of introgression lines of Solanum pimpinellifolium [J].Molecular Breeding,2014,34(4):1817-1831.
[21] 陆地棉TM-1背景的尖斑棉染色体片段渐渗系的培育研究[D].南京:南京农业大学,2014.
[22] Arbelaez J D,Moreno L T,Singh N,et al.Development and GBS-genotyping of introgression lines (ILs) using two wild species of rice,O.meridionalis and O.rufipogon,in a common recurrent parent,O.sativa cv.curinga [J].Molecular Breeding,2015,35(2):81.
[23] Tong V G.番茄InDel分子标记的开发与果实心室数量主效QTLlcn2.2的精细定位[D].北京:中国农业科学院,2016.
[24] 潘颖.利用高代回交群体定位醋栗番茄发芽期与幼苗期耐盐QTL及耐盐种质的创制[D].北京:中国农业科学院,2009.
[25] Liu X,Geng X,Zhang H,et al.Association and genetic identification of loci for four fruit traits in tomato using InDel markers [J].Frontiers in Plant Science,2017,8.
[26] Young N D,Tanksley S D.Restriction fragment length polymorphism maps and the concept of graphical genotypes [J].Theoretical & Applied Genetics,1989,77(1):95-101.
[27] Van B R.GGT 2.0:versatile software for visualization and analysis of genetic data [J].Journal of Heredity,2008,99(2):232-236.
[28] Ashrafi H,Kinkade M,Foolad M R.A new genetic linkage map of tomato based on a Solanum lycopersicum × S.pimpinellifolium RIL population displaying locations of candidate pathogen response genes [J].Genome,2009,52(11):935-956.
[29] Sharma A,Zhang L,Niňoliu D,et al.A Solanum lycopersicum × Solanum pimpinellifolium linkage map of tomato displaying genomic locations of R-genes,RGAs,and candidate resistance/defense-response ESTs [J].International Journal of Plant Genomics,2014,2008(4):926090.
[30] Zhou R,Yu X,Ottosen C O,et al.Drought stress had a predominant effect over heat stress on three tomato cultivars subjected to combined stress [J].Bmc Plant Biology,2017,17(1):24.
[31] 李学峰.野生黄瓜代换系的构建[D].北京:中国农业科学院,2010.
[32] 曾瑞珍,Talukdar A,刘芳,等.利用单片段代换系定位水稻粒形QTL[J].中国农业科学,2006,39(4):647-654.
[33] 余文贵,赵统敏,沈新莲,等.野生番茄单片段渐渗系的创建及应用[J].园艺学报,2006,33(1):197-202.
[34] 张桂权,曾瑞珍,丁效华,等.水稻单片段代换系文库的构建及其在分子育种上的应用[C].植物分子育种国际学术研讨会.2005.
[35] Allen K D,Sussex I M.Falsiflora and anantha control early stages of floral meristem development in tomato (Lycopersicon esculentum Mill.) [J].Planta,1996,200(2):254-264.
[36] Molinero-Rosales N,Latorre A,Jamilena M,et al.SINGLE FLOWER TRUSS regulates the transition and maintenance of flowering in tomato [J].Planta,2004,218(3):427-434.
[37] Pnueli L.The SELF-PRUNING gene of tomato regulates vegetative to reproductive switching of sympodial meristems and is the ortholog of CEN and TFL1 [J].Development,1998,125(11):1979-1989.
[38] Lifschitz E,Ayre B G,Eshed Y.Florigen and anti-florigen:a systemic mechanism for coordinating growth and termination in flowering plants [J].Frontiers in Plant Science,2014,5.
[39] 田小琴.番茄花序间隔节位的QTL定位及ShHsfC1的功能分析[D].武汉:华中农业大学,2018.
[40] 位江静,田丽萍,魏亦农,等.加工番茄早熟性状的相关性分析及QTL定位[J].北方园艺,2010(10):8-11.
[41] 李景富,彭艳,许向阳,等.番茄早熟性及QTL定位的研究[J].东北农业大学学报,2015,46(6).
[42] 刘杨,陈火英.利用重组自交系群体进行产量相关性状的QTL定位[C]//中国园艺学会.2008园艺学进展(第八辑):中国园艺学会第八届青年学术讨论会暨现代园艺论坛论文集.中国园艺学会,2008.2.
[43] Tanksley S D,Nelson J C.Advanced backcross QTL analysis:a method for the simultaneous discovery and transfer of valuable QTLs from unadapted germplasm into elite breeding lines [J].Theoretical and Applied Genetics,1996,92(2):191-203.