低温冷藏对番红花花芽中碳水化合物和氨基酸含量的影响
|
摘要
以番红花鳞茎为试材,进行不同变温处理,采用蒽酮比色法、3,5-二硝基水杨酸比色法和高速氨基酸自动分析仪,测定不同时间段花芽内可溶性糖、淀粉、淀粉酶和游离氨基酸含量,探讨低温冷藏与番红花发芽休眠解除之间的关系。结果表明:处理30 d后,可溶性糖含量急剧上升,淀粉含量急剧下降,α-淀粉酶和β-淀粉酶活性均表现出与可溶性糖含量相似的变化趋势;5℃/10℃处理30 d后花芽中可溶性蛋白质含量显著上升,精氨酸和脯氨酸含量显著下降;3个处理的谷氨酸含量均呈现整体上升趋势;多项测定指标之间呈显著或极显著相关。由此可知,番红花花芽经30 d低温处理后开始解除休眠,且以5℃/10℃处理效果最佳。
The bulbs of Crocussativus were subjected to different poikilothermic treatment.The methods of antorone colorimetry and 3,5-dinitrosalicylic acid colorimetry,and high speed amino acid analyzer were used to study the changes of soluble sugar,starch,amylase and free amino acids contents,to discuss the relationship of break dormancy between low-temperature storage and flower buds of C.sativus.The results showed that the contents of soluble sugar showed rapidly rising trend,and starch showed rapidly falling trend.The activities of α-amylase and β-amylase showed the similar trends to the contents of soluble sugar after 30 days treatment.The contents of soluble sugar showed the rapidly rising trend.Arginine and proline content showed the rapidly falling trend after 30 days treatment with 5 ℃/10 ℃,and glutamic acid content showed the rapidly rising trend after 3 different treatments.The correlation between indicators was significantly or highly significant.To sum up,the flower buds of C.sativus began to break dormancy after 30 days low-temperature treatment,and the treatment effect of 5 ℃/10 ℃ was the best.
The bulbs of Crocussativus were subjected to different poikilothermic treatment.The methods of antorone colorimetry and 3,5-dinitrosalicylic acid colorimetry,and high speed amino acid analyzer were used to study the changes of soluble sugar,starch,amylase and free amino acids contents,to discuss the relationship of break dormancy between low-temperature storage and flower buds of C.sativus.The results showed that the contents of soluble sugar showed rapidly rising trend,and starch showed rapidly falling trend.The activities of α-amylase and β-amylase showed the similar trends to the contents of soluble sugar after 30 days treatment.The contents of soluble sugar showed the rapidly rising trend.Arginine and proline content showed the rapidly falling trend after 30 days treatment with 5 ℃/10 ℃,and glutamic acid content showed the rapidly rising trend after 3 different treatments.The correlation between indicators was significantly or highly significant.To sum up,the flower buds of C.sativus began to break dormancy after 30 days low-temperature treatment,and the treatment effect of 5 ℃/10 ℃ was the best.
引文
[1] 国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2015.
[2] HILL T.The contemporary encyclopedia of herbs and species:seasonings for the global kitchen[M].Hoboken:John Wiley & Sons,Inc,2004.
[3] CAIOLA M G,CAPUTO P,ZANIER R.RAPD analysis in Crocus saivus L.accessions and related Crocus species[J].Biology Plantarum,2004,48:375-380.
[4] 周珠扬,强小林,朱磊,等.藏红花人工栽培与种球繁育技术[J].西藏科技,2011(2):25,31.
[5] LANGEN C M,HORT T,CROE T,et al.Domancy breaking in lily bulblets regenerated in vitro:Effects on growth after planting[J].Acta Horticulturae,1997,430:429-436.
[6] STUART N W.Effects of storage temperature on the forcing response of Easter lily and bulbous iris[A].Rep 13th Intl Hort Congr P,1952:514-524.
[7] 罗丽兰,石雷,张金政.低温对解除百合鳞茎休眠和促进开花的作用[J].园艺学报,2007,34(2):517-524.
[8] 黄敏玲,陈诗林,吴建设,等.低温和植物生长调节剂对荷兰鸢尾开花的效应[J].中南林学院学报,2003,23(5):34-37.
[9] 张志良,翟伟菁.植物生理学实验指导[M].北京:高等教育出版社,2009.
[10] 王学奎.植物生理生化实验原理和技术[M].北京:高等教育出版社,2006:174-176.
[11] REES A,CHARLES-EDWARDS D A.A simple model for the cold requirement of the tulip[J].Annals of Botany,1974,38(155):401-408.
[12] SHIN K S,CHAKRABARTY D,PAEK K Y.Sprouting rate change of carbohydrate contents and related enzymes during cold treatment of lily bulblets regenerated in vetro[J].Scientia Horticulturae,2002,96:195-204.
[13] DAVIES J N,KEMPTON R J.Carbohydrate changes in tulip bulbs during storage and forcing[J].Acta Horticulturae,1975,47:353-363.
[14] TZAY-FA S.Effect of day and night temperature variation and of high temperature on devernalization in radish[J].Acta Horticulturae,2000,514:157-162.
[15] 侯玲玲,夏宜平,何桂芳.低温作用影响百合鳞茎休眠的研究进展[J].青海大学学报(自然科学版),2006,24(2):60-62.
[16] KRUMPELMAN P M,FREYERMUTH S K,CANNON J F,et al.Nucleotide sequence of Arabidopsis thaliana arginase expressed in yeast[J].Plant Physiology,1995,107:1479-1480.
[17] 孙红梅,李天来,李云飞.低温解除休眠过程中兰州百合中部鳞片物质变化的生理机制[J].中国农业科学,2005,38(2):376-382.
[2] HILL T.The contemporary encyclopedia of herbs and species:seasonings for the global kitchen[M].Hoboken:John Wiley & Sons,Inc,2004.
[3] CAIOLA M G,CAPUTO P,ZANIER R.RAPD analysis in Crocus saivus L.accessions and related Crocus species[J].Biology Plantarum,2004,48:375-380.
[4] 周珠扬,强小林,朱磊,等.藏红花人工栽培与种球繁育技术[J].西藏科技,2011(2):25,31.
[5] LANGEN C M,HORT T,CROE T,et al.Domancy breaking in lily bulblets regenerated in vitro:Effects on growth after planting[J].Acta Horticulturae,1997,430:429-436.
[6] STUART N W.Effects of storage temperature on the forcing response of Easter lily and bulbous iris[A].Rep 13th Intl Hort Congr P,1952:514-524.
[7] 罗丽兰,石雷,张金政.低温对解除百合鳞茎休眠和促进开花的作用[J].园艺学报,2007,34(2):517-524.
[8] 黄敏玲,陈诗林,吴建设,等.低温和植物生长调节剂对荷兰鸢尾开花的效应[J].中南林学院学报,2003,23(5):34-37.
[9] 张志良,翟伟菁.植物生理学实验指导[M].北京:高等教育出版社,2009.
[10] 王学奎.植物生理生化实验原理和技术[M].北京:高等教育出版社,2006:174-176.
[11] REES A,CHARLES-EDWARDS D A.A simple model for the cold requirement of the tulip[J].Annals of Botany,1974,38(155):401-408.
[12] SHIN K S,CHAKRABARTY D,PAEK K Y.Sprouting rate change of carbohydrate contents and related enzymes during cold treatment of lily bulblets regenerated in vetro[J].Scientia Horticulturae,2002,96:195-204.
[13] DAVIES J N,KEMPTON R J.Carbohydrate changes in tulip bulbs during storage and forcing[J].Acta Horticulturae,1975,47:353-363.
[14] TZAY-FA S.Effect of day and night temperature variation and of high temperature on devernalization in radish[J].Acta Horticulturae,2000,514:157-162.
[15] 侯玲玲,夏宜平,何桂芳.低温作用影响百合鳞茎休眠的研究进展[J].青海大学学报(自然科学版),2006,24(2):60-62.
[16] KRUMPELMAN P M,FREYERMUTH S K,CANNON J F,et al.Nucleotide sequence of Arabidopsis thaliana arginase expressed in yeast[J].Plant Physiology,1995,107:1479-1480.
[17] 孙红梅,李天来,李云飞.低温解除休眠过程中兰州百合中部鳞片物质变化的生理机制[J].中国农业科学,2005,38(2):376-382.