RP-HPLC法测定17批不同产地苍术中苍术素的含量
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摘要
目的采用RP-HPLC法建立茅苍术中苍术素含量的测定方法,并对17批不同产地茅苍术药材中苍术素含量进行分析,为评价茅苍术药材质量提供参考。方法采用Agilent Eclipse XDB-C18(250 mm×4.6 mm,5μm)色谱柱;以乙腈-水(65∶35,V/V)为流动相;流速1.0 mL·min~(-1);柱温25℃;检测波长340 nm。结果方法学考察表明:苍术素浓度在0.705～42.3μg·mL~(-1)的范围内与峰面积呈良好的线形关系(r=0.9997),回归方程为Y=243188X-31.843,平均加样回收率为99.15%,RSD为1.06%(n=6),建立的方法重复性、耐用性、准确度良好。结论对17批样品进行分析,结果显示不同产地茅苍术中苍术素的含量差异较大,河南省方城县和嵩县的茅苍术中苍术素的含量明显高于其他产地,本实验为茅苍术药材的质量评价和控制提供了一定的科学依据。
Object In order to evaluate the quality of Atractylodes lancea,RP-HPLC method was established to determinate the content of atractylodin in 17 batches of Atraxtylodi Rhizoma from different habitats.Methods The determination was performed on Agilent Eclipse XDB-C18(250 mm×4.6 mm,5 μm) column with mobile phase consisted of acetonitrile-water(65∶35,V/V) at the flow rate of 1 mL·min~(-1).The detection wavelength was 340 nm,the colum temperature was 25 ℃.Results The methodological evaluation showed that there was good linear relationship for atractylodin in range of 0.705～42.3 μg·mL~(-1)(r=0.9997),the regression equation was Y=243188 X-31.843,the average recovery rate was 99.15%,RSD=1.06%(n=6).The developed method had good reproducibility,robustness and accuracy.Conclusion The analysis of 17 batches of samples exhibited that there were significant different among contents of atractylodin in Atractylodes lancea from different habitats.The results showed that contents of atractylodin in Atractylodes lancea from Fangcheng county and Songshan county of Henan province were apparently higher than that of other areas.This experiment provides a scientific basis for evaluation and control of the quality of Atractylodes lancea.
Object In order to evaluate the quality of Atractylodes lancea,RP-HPLC method was established to determinate the content of atractylodin in 17 batches of Atraxtylodi Rhizoma from different habitats.Methods The determination was performed on Agilent Eclipse XDB-C18(250 mm×4.6 mm,5 μm) column with mobile phase consisted of acetonitrile-water(65∶35,V/V) at the flow rate of 1 mL·min~(-1).The detection wavelength was 340 nm,the colum temperature was 25 ℃.Results The methodological evaluation showed that there was good linear relationship for atractylodin in range of 0.705～42.3 μg·mL~(-1)(r=0.9997),the regression equation was Y=243188 X-31.843,the average recovery rate was 99.15%,RSD=1.06%(n=6).The developed method had good reproducibility,robustness and accuracy.Conclusion The analysis of 17 batches of samples exhibited that there were significant different among contents of atractylodin in Atractylodes lancea from different habitats.The results showed that contents of atractylodin in Atractylodes lancea from Fangcheng county and Songshan county of Henan province were apparently higher than that of other areas.This experiment provides a scientific basis for evaluation and control of the quality of Atractylodes lancea.
引文
[1]国家药典委员会.中华人民共和国药典:一部[S].北京:中国医药科技出版社,2015:161-162.
[2]付梅红,朱东海,方婧,等.苍术的化学、分子生药学和药理学研究进展[J].中国中药杂志,2009,34(20):2669-672.
[3]邓爱平,李颖,吴志涛,等.苍术化学成分和药理的研究进展[J].中国中药杂志,2016,41(21):3904-3913.
[4]欧阳臻,江涛涛,缪亚东,等.苍术的化学成分、道地性和药理活性研究进展[J].时珍国医国药,2006,17(10):1936-1938.
[5]毛坤,向丽娟,张虎,等.湖北茅苍术挥发性化学成分的研究[J].时珍国医国药,2014,25(11):2622-2624.
[6]郭兰萍,黄璐琦,阎洪,等.基于地理信息系统的苍术道地药材气候生态特征研究[J].中国中药杂志,2005,30(8):565-569.
[7]丁宝章,王遂义.河南植物志第四册[M].郑州:河南科学技术出版社,1997:679.
[8]桂延耀,黄显章.南阳市第四次中药资源普查成果汇编[M].郑州:中州古籍出版社,2017:3-9.
[9]陈炎明,侴桂新,王峥涛,等.反相高效液相色谱法同时测定苍术中的两种聚乙炔类化合物[J].色谱,2007,25(1):84-87.
[10]张晓兰,李锦,李遇伯,等.高效液相色谱法测定茅苍术药材中β-桉叶醇与苍术素的含量[J].药物分析杂志,2009,29(12):2051-2054.
[11]方成武,樊敏,刘守金,等.HPLC测定不同居群苍术中苍术素醇的含量[J].中国中药杂志,2010,35(7):834-836.
[12]张磊,欧阳臻,赵明,等.茅苍术中苍术酮、茅术醇、β-桉叶醇和苍术素的同时含量测定及其聚类分析[J].中国中药杂志,2010,35(6):725-728.
[13]张金龙,秦昆明,徐自升,等.南北苍术炮制前后特征图谱及苍术素含量研究[J].科学技术与工程,2011,11(20):4843-4847.
[14]陈佳,解小霞,刘合刚.几个道地产区苍术指纹图谱及苍术素含量测定研究[J].中国实验方剂学杂志,2013,19(10):125-127.
[15]王爱妮,刘玉强,才谦.3种苍术的特征图谱及苍术素醇、苍术素和白术内酯Ⅱ含量测定研究[J].药物分析杂志,2016,36(1):91-95.
[16]李万娟,郭艳玲,商春丽,等.北苍术苍术素、苍术酮、β-桉叶醇测定及特征图谱研究[J].中草药,2016,47(2):330-335.
[17]陈文婷,陈阳,崔波,等.气相色谱“一测多评”用于苍术多成分含量测定的可行性研究[J].中国中药杂志,2018,43(3):551-556.
[18]李强,姚霞,孙楷,等.不同光质对茅苍术生长、抗氧化酶活性及挥发油含量的影响[J].中国实验方剂学杂志,2018,24(10):31-37.
[2]付梅红,朱东海,方婧,等.苍术的化学、分子生药学和药理学研究进展[J].中国中药杂志,2009,34(20):2669-672.
[3]邓爱平,李颖,吴志涛,等.苍术化学成分和药理的研究进展[J].中国中药杂志,2016,41(21):3904-3913.
[4]欧阳臻,江涛涛,缪亚东,等.苍术的化学成分、道地性和药理活性研究进展[J].时珍国医国药,2006,17(10):1936-1938.
[5]毛坤,向丽娟,张虎,等.湖北茅苍术挥发性化学成分的研究[J].时珍国医国药,2014,25(11):2622-2624.
[6]郭兰萍,黄璐琦,阎洪,等.基于地理信息系统的苍术道地药材气候生态特征研究[J].中国中药杂志,2005,30(8):565-569.
[7]丁宝章,王遂义.河南植物志第四册[M].郑州:河南科学技术出版社,1997:679.
[8]桂延耀,黄显章.南阳市第四次中药资源普查成果汇编[M].郑州:中州古籍出版社,2017:3-9.
[9]陈炎明,侴桂新,王峥涛,等.反相高效液相色谱法同时测定苍术中的两种聚乙炔类化合物[J].色谱,2007,25(1):84-87.
[10]张晓兰,李锦,李遇伯,等.高效液相色谱法测定茅苍术药材中β-桉叶醇与苍术素的含量[J].药物分析杂志,2009,29(12):2051-2054.
[11]方成武,樊敏,刘守金,等.HPLC测定不同居群苍术中苍术素醇的含量[J].中国中药杂志,2010,35(7):834-836.
[12]张磊,欧阳臻,赵明,等.茅苍术中苍术酮、茅术醇、β-桉叶醇和苍术素的同时含量测定及其聚类分析[J].中国中药杂志,2010,35(6):725-728.
[13]张金龙,秦昆明,徐自升,等.南北苍术炮制前后特征图谱及苍术素含量研究[J].科学技术与工程,2011,11(20):4843-4847.
[14]陈佳,解小霞,刘合刚.几个道地产区苍术指纹图谱及苍术素含量测定研究[J].中国实验方剂学杂志,2013,19(10):125-127.
[15]王爱妮,刘玉强,才谦.3种苍术的特征图谱及苍术素醇、苍术素和白术内酯Ⅱ含量测定研究[J].药物分析杂志,2016,36(1):91-95.
[16]李万娟,郭艳玲,商春丽,等.北苍术苍术素、苍术酮、β-桉叶醇测定及特征图谱研究[J].中草药,2016,47(2):330-335.
[17]陈文婷,陈阳,崔波,等.气相色谱“一测多评”用于苍术多成分含量测定的可行性研究[J].中国中药杂志,2018,43(3):551-556.
[18]李强,姚霞,孙楷,等.不同光质对茅苍术生长、抗氧化酶活性及挥发油含量的影响[J].中国实验方剂学杂志,2018,24(10):31-37.