用含兔血浆纤维蛋白原的Baird-Parker快速检测金黄色葡萄球菌的效果评价
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摘要
目的评估含兔血浆纤维蛋白原Baird-Parker对金黄色葡萄球菌的检测效果。方法 44株金黄色葡萄球菌、30株其他葡萄球菌和实际样品按GB 4789.10—2016接种到含兔血浆纤维蛋白原Baird-Parker、CHROMagar显色培养基及普通Baird-Parker培养基上,培养后观察结果。结果 44株金黄色葡萄球菌在3种培养基上均呈现典型的菌落特征。30株其他葡萄球菌中2株猪葡萄球菌和2株松鼠葡萄球菌在含兔血浆纤维蛋白原Baird-Parker上阳性,在CHROMagar上2株猪葡萄球菌、2株松鼠葡萄球菌和3株表皮葡萄球菌显品红色,其他显蓝绿色或无色,在普通BairdParker上有7株阳性。24份样品中分离到4份阳性;含兔血浆纤维蛋白原Baird-Parker分离到4份阳性;CHROMagar分离到6份阳性,经确认阳性4份;普通Baird-Parker分离到11份,经确认阳性3份。结论含兔血浆纤维蛋白原的Baird-Parker在金黄色葡萄球菌检测中有更高的特异性,将分离和确认融合起来,检测更快速,是一种非常实用的培养基,值得在实际样品分离中推广应用。
Objective To evaluate the effect of Baird-Parker with rabbit plasma fibrinogen for the detection of Staphylococcus aureus. Methods Forty-four strains of S. aureus,30 other Staphylococcus,and other species were inoculated onto Baird-Parker with rabbit plasma fibrinogen,CHROMagar chromogenic medium and Baird-Parker conventional isolation medium according to GB 4789. 10—2016. Results All the 44 S. aureus produced typical colonies on the 3 media. Two Staphylococcus hyicus strains and two Staphylococcus sciuri strains of 30 other Staphylococcus exhibited positive on Baird-Parker with rabbit plasma fibrinogen. On CHROMagar,two Staphylococcus hyicus strains,two Staphylococcus sciuri strains and three Staphylococcus epidermidis strains produce mauve like S. aureus,the others produced blue-green colonies or colorless. Severn of the 30 other Staphylococcus were found positive on Baird-Parker. 4 suspected positive samples isolated by Baird-Parker with rabbit plasma fibrinogen from the 24 food samples were confirmed positive. 4 of the 6 suspected positive samples isolated by CHROMagar were confirmed positive. 3 of the 11 suspected positive samples isolated by Baird-Parker were confirmed positive. Conclusion Pure cultures and food matrix test results showed that Baird-Parker with rabbit plasma fibrinogen was more specific than the other chromogenic media and conventional media. It has the best accuracy in isolation and presumptive identification of S. aureus. Baird-Parker with rabbit plasma fibrinogen medium is a very practical medium and is worthy of popularization in actual sample separation.
Objective To evaluate the effect of Baird-Parker with rabbit plasma fibrinogen for the detection of Staphylococcus aureus. Methods Forty-four strains of S. aureus,30 other Staphylococcus,and other species were inoculated onto Baird-Parker with rabbit plasma fibrinogen,CHROMagar chromogenic medium and Baird-Parker conventional isolation medium according to GB 4789. 10—2016. Results All the 44 S. aureus produced typical colonies on the 3 media. Two Staphylococcus hyicus strains and two Staphylococcus sciuri strains of 30 other Staphylococcus exhibited positive on Baird-Parker with rabbit plasma fibrinogen. On CHROMagar,two Staphylococcus hyicus strains,two Staphylococcus sciuri strains and three Staphylococcus epidermidis strains produce mauve like S. aureus,the others produced blue-green colonies or colorless. Severn of the 30 other Staphylococcus were found positive on Baird-Parker. 4 suspected positive samples isolated by Baird-Parker with rabbit plasma fibrinogen from the 24 food samples were confirmed positive. 4 of the 6 suspected positive samples isolated by CHROMagar were confirmed positive. 3 of the 11 suspected positive samples isolated by Baird-Parker were confirmed positive. Conclusion Pure cultures and food matrix test results showed that Baird-Parker with rabbit plasma fibrinogen was more specific than the other chromogenic media and conventional media. It has the best accuracy in isolation and presumptive identification of S. aureus. Baird-Parker with rabbit plasma fibrinogen medium is a very practical medium and is worthy of popularization in actual sample separation.
引文
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[2]中华人民共和国卫生部.GB 4789.10—2016食品安全国家标准食品微生物学检验金黄色葡萄球菌检验[S].北京:中国标准出版社,2016.
[3]U.S.Food and Drug Administration.Bacteriological analytical manual(BAM)[S].USA:Food and Drug Administration,2015.
[4]International Organization for Standardization.ISO 6888-1:1999Microbiology of food and animal feeding stuffs-Horizontal method for the enumeration of coagulase-positive staphylococci(Staphylococcus aureus and other species)Part 1:Technique using BairdParker agar medium[S].Switzerland:International Organization for Standardization,1999.
[5]International Organization for Standardization.ISO 6888-1:1999Microbiology of food and animal feeding stuffs-Horizontal method for the enumeration of coagulase-positive staphylococci(Staphylococcus aureus and other species)Part 2:Technique using rabbit plasma fibrinogen agar medium[S].Switzerland:International Organization for Standardization,1999.
[6]蔡芷荷,卢勉飞,滕昆仑,等.金黄色葡萄球菌显色培养基检测效果评价[J].中国卫生检验杂志,2015,25(6):771-774,800.
[7]黄汝添,吴清平,张菊梅,等.金黄色葡萄球菌显色培养基研究进展[J].中国卫生检验杂志,2007,17(6):1148-1150.
[8]曹建平,韩丹,王健.食品中金黄色葡萄球菌定量检测方法比较研究[J].安徽农业科学,2015,43(8):242-243,245.
[9]Gaillot O,Wetsch M,Fortineau N,et al.Evaluation of CHROMagar Staph.aureus,a new chromogenic medium for isolation and presumptive identification of Staphylococcus aureus from human clinical specimens[J].J Clin Microbiol,2000,38(4):1587-1591.
[10]Flayhart D,Lema C,Borek A,et al.Comparison of the BBL CHROMagar Staph aureus agar medium to conventional media for detection of Staphylococcus aureus in respiratory samples[J].J Clin Microbiol,2004,42(8):3566-3569.
[11]John DP,Claire R,Lynne A,et al.Evaluation of S.aureus ID,a new chromogenic agar medium for detection of Staphylococcus aureus[J].J Clin Microbiol,2003,41(12):5695-5698.
[12]Foster G,Ross HM,Hutson RA,et al.Staphylococcus lutrae sp.nov.,a new coagulase-positive species isolated from otters[J].Int J Syst Bacteriol,1997,47:724-726.
[13]Fleurette J,Bes M,Brun Y,et al.Clinical isolates of Staphylococcus lugdunensis and S.schleiferi:bacteriological characteristics and susceptibility to antimicrobial agents[J].Res Microbiol,1989,140:107-118.
[14]容艳芬,李艳嫦,卢勉飞,等.兔血浆纤维蛋白原琼脂培养基技术与Baird-Parker琼脂培养基技术检测食品中金黄色葡萄球菌的比较[J].中国卫生检验杂志,2017,27(5):642-645,648.
[15]苏妙贞,曾晓琮,韩志杰,等.金黄色葡萄球菌定量检测中四种培养基的比较[J].食品安全导刊,2017(21):139-140.
[16]顾其芳,张红芝,朱颖莹,等.三种金黄色葡萄球菌选择性分离培养基的检测效果比较[J].上海预防医学,2018,30(1):69-73.
[2]中华人民共和国卫生部.GB 4789.10—2016食品安全国家标准食品微生物学检验金黄色葡萄球菌检验[S].北京:中国标准出版社,2016.
[3]U.S.Food and Drug Administration.Bacteriological analytical manual(BAM)[S].USA:Food and Drug Administration,2015.
[4]International Organization for Standardization.ISO 6888-1:1999Microbiology of food and animal feeding stuffs-Horizontal method for the enumeration of coagulase-positive staphylococci(Staphylococcus aureus and other species)Part 1:Technique using BairdParker agar medium[S].Switzerland:International Organization for Standardization,1999.
[5]International Organization for Standardization.ISO 6888-1:1999Microbiology of food and animal feeding stuffs-Horizontal method for the enumeration of coagulase-positive staphylococci(Staphylococcus aureus and other species)Part 2:Technique using rabbit plasma fibrinogen agar medium[S].Switzerland:International Organization for Standardization,1999.
[6]蔡芷荷,卢勉飞,滕昆仑,等.金黄色葡萄球菌显色培养基检测效果评价[J].中国卫生检验杂志,2015,25(6):771-774,800.
[7]黄汝添,吴清平,张菊梅,等.金黄色葡萄球菌显色培养基研究进展[J].中国卫生检验杂志,2007,17(6):1148-1150.
[8]曹建平,韩丹,王健.食品中金黄色葡萄球菌定量检测方法比较研究[J].安徽农业科学,2015,43(8):242-243,245.
[9]Gaillot O,Wetsch M,Fortineau N,et al.Evaluation of CHROMagar Staph.aureus,a new chromogenic medium for isolation and presumptive identification of Staphylococcus aureus from human clinical specimens[J].J Clin Microbiol,2000,38(4):1587-1591.
[10]Flayhart D,Lema C,Borek A,et al.Comparison of the BBL CHROMagar Staph aureus agar medium to conventional media for detection of Staphylococcus aureus in respiratory samples[J].J Clin Microbiol,2004,42(8):3566-3569.
[11]John DP,Claire R,Lynne A,et al.Evaluation of S.aureus ID,a new chromogenic agar medium for detection of Staphylococcus aureus[J].J Clin Microbiol,2003,41(12):5695-5698.
[12]Foster G,Ross HM,Hutson RA,et al.Staphylococcus lutrae sp.nov.,a new coagulase-positive species isolated from otters[J].Int J Syst Bacteriol,1997,47:724-726.
[13]Fleurette J,Bes M,Brun Y,et al.Clinical isolates of Staphylococcus lugdunensis and S.schleiferi:bacteriological characteristics and susceptibility to antimicrobial agents[J].Res Microbiol,1989,140:107-118.
[14]容艳芬,李艳嫦,卢勉飞,等.兔血浆纤维蛋白原琼脂培养基技术与Baird-Parker琼脂培养基技术检测食品中金黄色葡萄球菌的比较[J].中国卫生检验杂志,2017,27(5):642-645,648.
[15]苏妙贞,曾晓琮,韩志杰,等.金黄色葡萄球菌定量检测中四种培养基的比较[J].食品安全导刊,2017(21):139-140.
[16]顾其芳,张红芝,朱颖莹,等.三种金黄色葡萄球菌选择性分离培养基的检测效果比较[J].上海预防医学,2018,30(1):69-73.