巧克力中沙门氏菌检测能力验证结果与分析
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摘要
目的通过参加中国食品药品检定研究院组织的NIFDC-PT-135巧克力中沙门氏菌检验能力验证活动,提高实验室沙门氏菌的检测能力,增强实验室的竞争力。方法按照GB 4789.10-2016《食品安全国家标准食品微生物学检验沙门氏菌检验》进行沙门氏菌的分离与血清学鉴定,辅以VIDAS30全自动酶联免疫分析系统进行快速初筛,并以VITEK 2 Compact自动微生物快速检测分析系统对分离出的可疑菌落进行生化鉴定。结果样品编号为0148的检出鼠伤寒沙门氏菌, 0546的检出肠沙门菌双相亚利桑那亚种(沙门氏菌Ⅲb),0676未检出沙门氏菌。结论本次实验发现的肠沙门菌双相亚利桑那亚种在沙门氏菌属显色平板上出现蓝色菌落,与以往判定的紫红色菌落有差异,在检测中应当引起关注。
Objective To improve the detection capability of Salmonella and strengthen the competition ability of laboratory by participating in proficiency testing on Salmonella detection in chocolate of NIFDC-PT-135 organized by National Institutes for Food and Drug Control. Methods Isolation and serological identification of Salmonella were carried out according to GB 4789.4-2016 National food safety standard-Food microbiological examination-Salmonella, the VIDAS 30 automatic enzyme-linked immunoassay system was supplemented for rapid screening, and the biochemical identification of suspected isolated colonies was carried out by VITEK 2 Compact automatic microorganism rapid detection and analysis system. Results Salmonella typhimurium was detected in 0148, Salmonella enterica ssp diarizonae(Salmonella Ⅲb) was detected in 0546, and Salmonella spp. was not detected in 0676. Conclusion The blue colonies of the Salmonella enterica ssp diarizonae are found on the color plate of Salmonella in this experiment, which is different from the previous determination of purple red colonies, and should be concerned in the detection.
Objective To improve the detection capability of Salmonella and strengthen the competition ability of laboratory by participating in proficiency testing on Salmonella detection in chocolate of NIFDC-PT-135 organized by National Institutes for Food and Drug Control. Methods Isolation and serological identification of Salmonella were carried out according to GB 4789.4-2016 National food safety standard-Food microbiological examination-Salmonella, the VIDAS 30 automatic enzyme-linked immunoassay system was supplemented for rapid screening, and the biochemical identification of suspected isolated colonies was carried out by VITEK 2 Compact automatic microorganism rapid detection and analysis system. Results Salmonella typhimurium was detected in 0148, Salmonella enterica ssp diarizonae(Salmonella Ⅲb) was detected in 0546, and Salmonella spp. was not detected in 0676. Conclusion The blue colonies of the Salmonella enterica ssp diarizonae are found on the color plate of Salmonella in this experiment, which is different from the previous determination of purple red colonies, and should be concerned in the detection.
引文
[1]苏粉良,石建华,陈雨欣.食品能力验证样品中沙门氏菌的分离与鉴定[J].农产品加工,2015,(20):36-37,39.Su FL,Shi JH,Chen YX.Isolation and identification of Salmonella sp.during the proficiency testing[J].Farm Prod Proc,2015,(20):36-37,39.
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[3]王志伟,徐琼,陈欣钦,等.能力验证样品中沙门氏菌的分离与鉴定[J].食品研究与开发,2016,37(7):161-163.Wang ZW,Xu Q,Chen XQ,et al.Isolation and identification of Salmonella in the proficiency testing sample[J].Food Res Dev,2016,37(7):161-163.
[4]王超,吴亚英.一株沙门氏菌能力验证的鉴定与分析[J].世界最新医学信息文摘,2016,16(30):44-45.Wang C,Wu YY.Identification and analysis of a strain Salmonella in proficiency testing[J].World Latest Med Inf,2016,16(30):44-45.
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[6]鞠慧萍,孙鹏翔,苏粉良.食品能力验证样品中沙门氏菌的分离及鉴定[J].农产品加工学刊,2014,(8):48-49.Ju HP,Sun PX,Su FL.Isolation and identification of Salmonella spp.In food during the proficiency testing[J].Acad Period Farm Prod Proc,2014,(8):48-49.
[7]罗丽珠,陈婉娃,许小妹.能力验证中沙门氏菌的分离鉴定与血清分型[J].食品安全质量检测学报,2018,9(5):1117-1121.Luo LZ,Chen WW,Xu XM.Isolation and identification of Salmonella and serotype in proficiency testing[J].J Food Saf Qual,2018,9(5):1117-1121.
[8]史晓娟,马建敏,孙丽莉,等.食源性沙门氏菌鉴定和血清分型能力验证[J].食品安全质量检测学报,2018,9(7):1534-1537.Shi XJ,Ma JM,Sun LL,et al.Ability verification of identification and serotyping of foodbrone Salmonella[J].J Food Saf Qual,2018,9(7):1534-1537.
[9]黄翠薇,席静,梁瑞婷.浅析实验室参加能力验证活动的过程及意义[J].现代测量与实验室管理,2017,(6):59-60.Huang CW,Xi J,Liang RT.Analysis on the process and significance of laboratory participation ability verification[J].Adv Meas Lab Manag,2017,(6):59-60.
[10]唐爱明,王辉,夏延斌,等.食品微生物CNAS检测能力验证结果与分析[J].食品安全质量检测学报,2012,3(3):222-225.Tang AM,Wang H,Xia YB,et al.Proficiency testing results and analysis in food microbiological CNAS examination[J].J Food Saf Qual,2012,3(3):222-225.
[11]GB 4789.4-2016食品安全国家标准食品微生物学检验沙门氏菌检验[S].GB 4789.4-2016 National food safety standard-Food microbiological examination-Salmonella[S].
[12]陈雨欣,苏粉良,鞠慧萍,等.食品能力验证中沙门氏菌的分离鉴定与血清分型[J].食品安全质量检测学报,2016,7(7):2937-2941.Chen YX,Su FL,Ju HP,et al.Isolation and identification of Salmonella and serotype in food ability verification[J].J Food Saf Qual,2016,7(7):2937-2941.
[13]Antigenic Formulae of the Salmonella serovars,(9th ed.)Paris:WHOcollaborating centre for reference and research on Salmonella[Z].2007.
[14]翟平平,吴鑫,刘德,等.奶粉样品中沙门氏菌检测能力验证结果与分析[J].食品安全质量检测学报,2018,9(7):1518-1523.Zhai PP,Wu X,Liu D,et al.Proficiency testing results and analysis of detection of Salmonella in milk powder samples[J].J Food Saf Qual,2018,9(7):1518-1523.
[15]颜瑛,罗玉彬,王文娟,等.食品能力验证中沙门氏菌的分离和鉴定[J].食品安全质量检测学报,2015,6(9):3501-3502.Yan Y,Luo YB,Wang WJ,et al.Isolation and identification of Salmonella spp.in food during the proficiency testing[J].J Food Saf Qual,2015,6(9):3501-3502.
[16]陈茂义,胡婕,刘建昭,等.科马嘉显色培养基和XLD、SS、HE分离食品中沙门氏菌效果比较[J].公共卫生与预防医学,2008,19(4):12-14.Chen MY,Hu J,Liu JZ,et al.Comparision of CHROM agar Salmonella medium and XLD,SS agars and HE media for isolation of Salmonella strains from food samples[J].J Publ Health Prev Med,2008,19(4):12-14.
[17]江志杰,王似锦,高春.食品中沙门氏菌检出能力验证结果与分析[J].食品安全质量检测学报,2015,6(5):1932-1933.Jiang ZJ,Wang SJ,Gao C.Proficiency testing results and analysis of Salmonella detection ability in food[J].J Food Saf Qual,2015,6(5):1932-1933.
[2]高晗,何娟,严礼.3种沙门氏菌检测方法能力验证[J].食品安全质量检测学报,2017,8(2):513-514.Gao H,He J,Yan L.Capability verification of three detection methods of Salmonella[J].J Food Saf Qual,2017,8(2):513-514.
[3]王志伟,徐琼,陈欣钦,等.能力验证样品中沙门氏菌的分离与鉴定[J].食品研究与开发,2016,37(7):161-163.Wang ZW,Xu Q,Chen XQ,et al.Isolation and identification of Salmonella in the proficiency testing sample[J].Food Res Dev,2016,37(7):161-163.
[4]王超,吴亚英.一株沙门氏菌能力验证的鉴定与分析[J].世界最新医学信息文摘,2016,16(30):44-45.Wang C,Wu YY.Identification and analysis of a strain Salmonella in proficiency testing[J].World Latest Med Inf,2016,16(30):44-45.
[5]骆海朋,唐颂,陈怡文,等.沙门氏菌能力验证样品的研制及其应用[J].食品安全质量检测学报,2016,7(4):1473-1478.Luo HP,Tang S,Chen YW,et al.Preparation of quality control samples of Salmonella and their application in the proficiency test[J].J Food Saf Qual,2016,7(4):1473-1478.
[6]鞠慧萍,孙鹏翔,苏粉良.食品能力验证样品中沙门氏菌的分离及鉴定[J].农产品加工学刊,2014,(8):48-49.Ju HP,Sun PX,Su FL.Isolation and identification of Salmonella spp.In food during the proficiency testing[J].Acad Period Farm Prod Proc,2014,(8):48-49.
[7]罗丽珠,陈婉娃,许小妹.能力验证中沙门氏菌的分离鉴定与血清分型[J].食品安全质量检测学报,2018,9(5):1117-1121.Luo LZ,Chen WW,Xu XM.Isolation and identification of Salmonella and serotype in proficiency testing[J].J Food Saf Qual,2018,9(5):1117-1121.
[8]史晓娟,马建敏,孙丽莉,等.食源性沙门氏菌鉴定和血清分型能力验证[J].食品安全质量检测学报,2018,9(7):1534-1537.Shi XJ,Ma JM,Sun LL,et al.Ability verification of identification and serotyping of foodbrone Salmonella[J].J Food Saf Qual,2018,9(7):1534-1537.
[9]黄翠薇,席静,梁瑞婷.浅析实验室参加能力验证活动的过程及意义[J].现代测量与实验室管理,2017,(6):59-60.Huang CW,Xi J,Liang RT.Analysis on the process and significance of laboratory participation ability verification[J].Adv Meas Lab Manag,2017,(6):59-60.
[10]唐爱明,王辉,夏延斌,等.食品微生物CNAS检测能力验证结果与分析[J].食品安全质量检测学报,2012,3(3):222-225.Tang AM,Wang H,Xia YB,et al.Proficiency testing results and analysis in food microbiological CNAS examination[J].J Food Saf Qual,2012,3(3):222-225.
[11]GB 4789.4-2016食品安全国家标准食品微生物学检验沙门氏菌检验[S].GB 4789.4-2016 National food safety standard-Food microbiological examination-Salmonella[S].
[12]陈雨欣,苏粉良,鞠慧萍,等.食品能力验证中沙门氏菌的分离鉴定与血清分型[J].食品安全质量检测学报,2016,7(7):2937-2941.Chen YX,Su FL,Ju HP,et al.Isolation and identification of Salmonella and serotype in food ability verification[J].J Food Saf Qual,2016,7(7):2937-2941.
[13]Antigenic Formulae of the Salmonella serovars,(9th ed.)Paris:WHOcollaborating centre for reference and research on Salmonella[Z].2007.
[14]翟平平,吴鑫,刘德,等.奶粉样品中沙门氏菌检测能力验证结果与分析[J].食品安全质量检测学报,2018,9(7):1518-1523.Zhai PP,Wu X,Liu D,et al.Proficiency testing results and analysis of detection of Salmonella in milk powder samples[J].J Food Saf Qual,2018,9(7):1518-1523.
[15]颜瑛,罗玉彬,王文娟,等.食品能力验证中沙门氏菌的分离和鉴定[J].食品安全质量检测学报,2015,6(9):3501-3502.Yan Y,Luo YB,Wang WJ,et al.Isolation and identification of Salmonella spp.in food during the proficiency testing[J].J Food Saf Qual,2015,6(9):3501-3502.
[16]陈茂义,胡婕,刘建昭,等.科马嘉显色培养基和XLD、SS、HE分离食品中沙门氏菌效果比较[J].公共卫生与预防医学,2008,19(4):12-14.Chen MY,Hu J,Liu JZ,et al.Comparision of CHROM agar Salmonella medium and XLD,SS agars and HE media for isolation of Salmonella strains from food samples[J].J Publ Health Prev Med,2008,19(4):12-14.
[17]江志杰,王似锦,高春.食品中沙门氏菌检出能力验证结果与分析[J].食品安全质量检测学报,2015,6(5):1932-1933.Jiang ZJ,Wang SJ,Gao C.Proficiency testing results and analysis of Salmonella detection ability in food[J].J Food Saf Qual,2015,6(5):1932-1933.