富含脯氨酸的酪氨酸激酶2和磷酸化的丝氨酸/苏氨酸蛋白激酶在舌鳞状细胞癌组织中的表达及临床意义
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摘要
目的探讨富含脯氨酸的酪氨酸激酶2(Pyk2)和磷酸化的丝氨酸/苏氨酸蛋白激酶(p-AKT)在舌鳞状细胞癌(TSCC)及其癌旁非肿瘤组织中的表达差异及临床意义。方法采用免疫组织化学法检测45例TSCC组织及30例癌旁非肿瘤组织中Pyk2和p-AKT蛋白的表达情况,并分析两者蛋白表达与临床病理参数的关系。结果在TSCC组织中Pyk2和p-AKT蛋白均呈高表达,而在癌旁非肿瘤中呈低表达或无表达(P<0.05)。并且两者的表达呈正相关(γs=0.412)。Pyk2蛋白的表达与病理分化程度、有无淋巴结转移及TNM临床分期有关(P<0.05),与性别、年龄无关。而p-AKT只与病理分化程度有关(P<0.05)。结论 Pyk2和p-AKT蛋白异常表达与TSCC的发生、发展密切相关,联合检测两者有望成为明确TSCC恶性程度的指标。
Objective This study aimed to investigate the expression and clinical significance of proline-rich tyrosine kinase 2(Pyk2) and phospho-protein kinase B(p-AKT) in tongue squamous cell carcinoma(TSCC) and adjacent nontumor tissues. Methods The Pyk2 and p-AKT protein levels were detected via immunohistochemistry in 45 cases of TSCC tissues and 30 cases of adjacent nontumor tissues. The relationships of the two protein levels and clinicopathological characteristics were also analyzed. Results Pyk2 and p-AKT levels were significantly higher in the TSCC tissues than in the adjacent nontumor tissues(P<0.05). Nontumor tissues showed poor or no expression. The expression levels of the two proteins were positively correlated(γs=0.412). The expression of Pyk2 was associated with histopathological differentiation type, regional lymph node metastasis, and TNM staging(P<0.05), but not with age and gender. The expression of p-AKT was only related to histopathological differentiation types(P<0.05). Conclusion The abnormal expression of Pyk2 and p-AKT proteins might be closely related to the development and progression of TSCC. Joint detection can be used as an indicator to estimate the degree of TSCC.
Objective This study aimed to investigate the expression and clinical significance of proline-rich tyrosine kinase 2(Pyk2) and phospho-protein kinase B(p-AKT) in tongue squamous cell carcinoma(TSCC) and adjacent nontumor tissues. Methods The Pyk2 and p-AKT protein levels were detected via immunohistochemistry in 45 cases of TSCC tissues and 30 cases of adjacent nontumor tissues. The relationships of the two protein levels and clinicopathological characteristics were also analyzed. Results Pyk2 and p-AKT levels were significantly higher in the TSCC tissues than in the adjacent nontumor tissues(P<0.05). Nontumor tissues showed poor or no expression. The expression levels of the two proteins were positively correlated(γs=0.412). The expression of Pyk2 was associated with histopathological differentiation type, regional lymph node metastasis, and TNM staging(P<0.05), but not with age and gender. The expression of p-AKT was only related to histopathological differentiation types(P<0.05). Conclusion The abnormal expression of Pyk2 and p-AKT proteins might be closely related to the development and progression of TSCC. Joint detection can be used as an indicator to estimate the degree of TSCC.
引文
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[2]杨亮亮.PYK2在CCR7调控的头颈鳞癌的侵袭和迁移中的作用的研究[D].沈阳:中国医科大学,2012.Yang LL.Studies on the effects of PYK2 on the invasion and migration of CCR7 control of head and neck squamous cell carcinoma[D].Shenyang:China Medical University,2012.
[3]Stanzione R,Picascia A,Chieffi P,et al.Variations of proline-rich kinase Pyk2 expression correlate with prostate cancer progression[J].Lab Invest,2001,81(1):51-59.
[4]Sun CK,Ng KT,Sun BS,et al.The significance of prolinerich tyrosine kinase2(Pyk2)on hepatocellular carcinoma progression and recurrence[J].Br J Cancer,2007,97(1):50-57.
[5]Tomita N,Hayashi Y,Suzuki S,et al.Structure-based discovery of cellular-active allosteric inhibitors of FAK[J].Bioorg Med Chem Lett,2013,23(6):1779-1785.
[6]Gomez-Puerta JA,Mócsai A.Tyrosine kinase inhibitors for the treatment of rheumatoid arthritis[J].Curr Top Med Chem,2013,13(6):760-773.
[7]王德勇.P-Akt和P-m TOR在口腔鳞状细胞癌中的表达及临床意义[D].蚌埠:蚌埠医学院,2013.Wang DY.Expression and clinical significance of P-Akt and P-m TOR signal pathway proteins in oral squamous cell carcinoma[D].Bengbu:Bengbu Medical College,2013.
[8]魏凌云,郭明.Shh、Gli1和p-Akt在食管癌中的表达及临床意义[J].临床军医杂志,2014,42(12):1241-1245.Wei LY,Guo M.Expression of Shh,Gli1 and p-Akt in esophageal squamous cell carcinoma and their clinical significance[J].Clin J Med Offic,2014,42(12):1241-1245.
[9]董君,马彬,杨京京,等.结直肠癌中p-AKT和PTEN蛋白的表达及其临床意义[J].临床与实验病理学杂志,2010,26(3):316-319.Dong J,Ma B,Yang JJ,et al.Clinical significance of p-AKT and PTEN protein expression in colorectal carcinoma[J].J Clin Exp Pathol,2010,26(3):316-319.