带叶兜兰非共生萌发试验
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摘要
以带叶兜兰同种异株授粉的果实为试验材料,采用正交实验设计,研究了非共生条件对带叶兜兰种子萌发的影响,以期为带叶兜兰的快速繁殖并最终实现产业化生产提供技术参考。结果表明:基本培养基的种类是带叶兜兰非共生萌发的主要影响因素,初步筛选出种子非共生萌发较适宜培养基为1/2MS+1.5mg·L~(-1) 6-BA+0.6mg·L~(-1) NAA+0.75mg·L~(-1) AC+2.5%蔗糖+0.46%琼脂+100mL·L~(-1)椰汁;果实的成熟度影响种子萌发所历经的胚龄、原球茎的颜色及其后续发育,果龄过短或过长都不利于种子发育,带叶兜兰离体培养的最佳时间为果龄180d。
Taking the fruits of Paphiopedilumhirsutissimum from the allogeneic pollination as materials,under the condition of asymbiotic germination,the test had been done of the seed germination of P.hirsutissimumby the orthogonal test,in order to provide the basis of the rapid propagation and industrial production of P.hirsutissimum.The results showed that the asymbiotic germination of P.hirsutissimum was mostly influenced by the basic medium,and the better medium for asymbiotic germination was 1/2MS+1.5mg·L~(-1) 6-BA+0.6mg·L~(-1) NAA+0.75mg·L~(-1) AC+2.5%sucrose+0.46%agar+100mL·L~(-1) coconut juice;the embryo ages,the protocorm color and the protocorm growth were affected by the fruit maturity,the fruit age was harmful to the seed germination,which was too young or too old,and the180-day-old fruit was the optimal in vitro cultivation of P.hirsutissimum.
Taking the fruits of Paphiopedilumhirsutissimum from the allogeneic pollination as materials,under the condition of asymbiotic germination,the test had been done of the seed germination of P.hirsutissimumby the orthogonal test,in order to provide the basis of the rapid propagation and industrial production of P.hirsutissimum.The results showed that the asymbiotic germination of P.hirsutissimum was mostly influenced by the basic medium,and the better medium for asymbiotic germination was 1/2MS+1.5mg·L~(-1) 6-BA+0.6mg·L~(-1) NAA+0.75mg·L~(-1) AC+2.5%sucrose+0.46%agar+100mL·L~(-1) coconut juice;the embryo ages,the protocorm color and the protocorm growth were affected by the fruit maturity,the fruit age was harmful to the seed germination,which was too young or too old,and the180-day-old fruit was the optimal in vitro cultivation of P.hirsutissimum.
引文
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[2]王晓国,卢家仕,周主贵,等.带叶兜兰菌根真菌分离和初步鉴定[J].西南农业学报,2016,29(2):316-320.
[3]曾宋君,陈之林,段俊.带叶兜兰的无菌播种和离体快速繁殖[J].植物生理学通讯,2006,42(2):247.
[4]尤佳妍.几种兜兰的种子发育及无菌萌发研究[D].北京:北京林业大学,2014.
[5]陈之林,叶秀粦,梁承邺,等.杏黄兜兰和硬叶兜兰的种子试管培养[J].园艺学报,2004,31(4):540-542.
[6]丁长春,李蕾,夏念和.硬叶兜兰的无菌播种和试管成苗[J].北方园艺,2011(5):115-117.
[7]刘其府,傅燕艳,曾宋君,等.同色兜兰种子非共生萌发试验[J].广东农业科学,2012(12):47-49.
[8]周丽,邓克云,徐正海.‘金州报春’兜兰的非共生萌发与快速繁[J].植物生理学通讯,2013,49(11):1250-1254.
[9]周艳,周洪英,朱立,等.硬叶兜兰的无菌播种和离体快速繁殖[J].贵州科学,2013,31(5):79-82.
[10]尤佳妍,张毓,刘岩,等.小叶兜兰的种子发育和无菌萌发[J].植物生理学报,2014,50(3):275-282.
[11]ROY A R,PATEL R S,PATEL V V,et al.Asymbiotic seed germination,mass propagation and seedling development of Vanda coerulea Griff ex.Lindl.(Blue Vanda):An in vitro protocol for an endangered orchid[J].Scientia Horticulturae,2011,128:325-331.
[12]ABRAHAM S,AUGUSTINE J,THOMAS T D.Asymbiotic seed germination and in vitro conservation of Coelogyne nervosa A.Rich.an endemic orchid to Western Ghats[J].Physiological and Molecular Biology of Plants,2012,18(3):245-251.
[13]SUZUKI R M,MOREIRA V C,PESCADOR R,et al.Asymbiotic seed germination and in vitro seedling development of the threatened orchid Hoffmannseggella cinnabarina[J].In Vitro Cellular Developmental Biology Plant,2012,48:500-511.
[14]BEKTAS E,CUCE M,SOKMEN A.In vitro germination,protocorm formation,and plantlet development of Orchis coriophora(Orchidaceae),a naturally growing orchid species in Turkey[J].Turkish Journal of Botany,2013,37:336-342.
[15]ARDITTI J,ERNST R.Micro propagation of Orchids[M].New York:J Wiley and Sons,Inc,1993.
[16]丁长春,虞泓,刘方媛.影响杏黄兜兰种子萌发的因素[J].云南植物研究,2004,26(6):673-677.
[17]陈莹,范旭丽,高江去.白旗兜兰种子非共生萌发与试管苗快速繁育[J].植物分类与资源学报,2015,37(5):611-615.
[18]王亚平,谭志勇,张乐萍,等.兜兰无菌播种技术研究[J].现代农业科技,2012(18):157,171.