铁皮石斛生根组培苗污染后的处理和利用
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摘要
铁皮石斛(Dendrobium officinale)是名贵中药材,人工种植面积的扩大造成种苗短缺。为降低规模化育苗过程中的种苗生产成本,提高污染苗的利用率,对铁皮石斛组培污染苗的利用进行试验研究,结果表明,氨基寡糖素1 000倍液可有效提高污染苗的移栽成活率,增加发芽率,减少发病率;结合1 500倍多菌灵液双重使用,可促使根的生长发育,植株茎粗壮,萌芽多。
Dendrobium officinale is one of the precious chinese medicines. With the expansion of artificial planting,seedlings were short. To reduce the production cost in the process of large scaled rearing and increase the utilization of polluted tissue-cultured plants,utilization of polluted tissue-cultured plants was studied. The results showed that the survival ratio and buds of polluted plants increased significantly,but morbidity dropped significantly when the plants were irrigated by oligosaccharins with 1 000 times,which mixed with sanmate with 1 500 times could promote the root growth,and make the plant stem strong and sprout more.
Dendrobium officinale is one of the precious chinese medicines. With the expansion of artificial planting,seedlings were short. To reduce the production cost in the process of large scaled rearing and increase the utilization of polluted tissue-cultured plants,utilization of polluted tissue-cultured plants was studied. The results showed that the survival ratio and buds of polluted plants increased significantly,but morbidity dropped significantly when the plants were irrigated by oligosaccharins with 1 000 times,which mixed with sanmate with 1 500 times could promote the root growth,and make the plant stem strong and sprout more.
引文
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[2]LI Y,ZHAO Y P,CHEN P Y,et al.The scavenging oxygen free-radical effects of water extract from five Kinds of Dendrobium candidum[J].Chin Tradit Herb Drugs,2004,35(11):1240-1242.
[3]陈丽新,苏国秀,苏宾,等.香蕉组培苗生产中污染苗的利用研究[J].亚热带植物科学,2001,30(4):18-20.
[4]狄维,王林,王升启.寡糖及其衍生物的生物活性研究进展[J].中国药物化学杂志,2002,12(4),243-248.
[5]赵继红,孙淑君,李建中.植物诱导抗病性与诱抗剂研究进展[J].植物保护,2003,29(4):7-10.
[6]薛改妮,张宝强.氨基寡糖素在小麦上的应用效果研究[J].现代农业科技,2012(18):97-98.
[7]陆健生.0.5%氨基寡糖素水剂防治棉花苗期枯萎病试验初报[J].中国棉花,2009,36(2):24-25.
[8]武清彪,李爱萍.5%海岛素水剂在甜椒上应用效果初探[J].农业技术与装备,2012(6):53-55.
[9]陈旭辉,彭睿,雷小春,等.海岛素在中晚稻和蔬菜增产作用示范[J].湖北植保,2012(3):31-32.
[10]李萍,张善学,李国梁,等.氨基寡糖素在豇豆上的应用效果[J].中国植物导刊,2013(7):48-51.
[11]陈卫民,钟宁,杨飞.海绿素对全球红葡萄品质影响[J].北方园艺,2010(17):53-54.
[12]王廷芹.海绿素对苋菜生物学特性及品质和产量的影响[J].北方园艺,2012(16):146-150.
[13]余中莲.氨基寡糖素诱导荔枝果实对霜疫霉病的抗性[D].福州:福建农林大学,2012.
[14]苏小记,贾丽娜.2.0%氨基寡糖素水剂防治烟草病毒病药效试验[J].陕西农业科学,2005(3):55-56.
[15]苏小记,王亚红,贾丽娜.氨基寡糖素对辣椒病害的控制作用研究[J].中国农学通报,2004,20(2):195-197.
[16]苏小记,王亚红,贾丽娜,等.氨基寡糖素对番茄主要病害的防治作用[J].西北农业学报,2004,13(2):79-82.
[17]张敬泽,郑小军.铁皮石斛黑斑病病原菌的鉴定和侵染过程的细胞学研究[J].植物病理学报,2004,34(1):92-94.
[18]李静,张敬泽,吴晓鹏,等.铁皮石斛疫病及其病原菌[J].菌物学报,2008,27(2):171-176.