芩柏汤对溃疡性结肠炎大鼠病理损伤的干预研究
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摘要
目的:研究芩柏汤对溃疡性结肠炎大鼠结肠病理变化的影响,探讨其治疗效果及作用机制。方法:运用复合法(三硝基苯磺酸+乙醇)制备细胞免疫反应性UC大鼠模型,大鼠随机分成正常对照组、UC模型组、美沙拉嗪组和芩柏汤高、中、低剂量组。保留灌肠治疗3周后评估各组大鼠饮食量、体质量变化及组织损伤程度;采用ELISA法测定大鼠血清中TNF-α、IL-6、IL-17含量。结果:模型组大鼠饮食量及体质量明显低于正常组,血清中TNF-α、IL-6、IL-17水平、结肠组织损伤程度明显高于正常组。芩柏汤高、中、低剂量组大鼠上述指标均较模型组有不同程度地好转,且呈一定的量效关系。结论:芩柏汤对溃疡性结肠炎的治疗作用可能是通过下调IL-17的表达,进而下调炎性因子的表达实现改善溃疡性结肠炎病理变化。
Objective: To study the effect of Qinbai decoction on pathological changes in rats with ulcerative colitis(UC), and to explore its therapeutic effect and mechanism. Methods: The compound method(three nitrobenzene sulfonic acid + ethanol) was used to prepare the cell immunoreactive UC rat model, and the rats were randomly divided into normal control group, model group, mesalazine group and Qinbai decoction high,medium and low dose group. After 3 weeks of retention enema, the diet, body weight and tissue damage of rats in each group were evaluated. The levels of TNF-α, IL-6 and IL17 were determined by ELISA. Results: The model group showed lower diet and weight than those the normal group, while higher level of TNF-α, IL-6,IL17 and the damage of the colon tissue in the serum than the normal group. The above indexes in the high,middle and low dose groups of the Qinbai decoction were better than those in the model group, and showed a dose-dependent relationship. Conclusion: The therapeutic effect of Qinbai decoction on ulcerative colitis may be through the expression of IL17, and then down-regulation of the expression of inflammatory factors to improve the pathological changes of ulcerative colitis.
Objective: To study the effect of Qinbai decoction on pathological changes in rats with ulcerative colitis(UC), and to explore its therapeutic effect and mechanism. Methods: The compound method(three nitrobenzene sulfonic acid + ethanol) was used to prepare the cell immunoreactive UC rat model, and the rats were randomly divided into normal control group, model group, mesalazine group and Qinbai decoction high,medium and low dose group. After 3 weeks of retention enema, the diet, body weight and tissue damage of rats in each group were evaluated. The levels of TNF-α, IL-6 and IL17 were determined by ELISA. Results: The model group showed lower diet and weight than those the normal group, while higher level of TNF-α, IL-6,IL17 and the damage of the colon tissue in the serum than the normal group. The above indexes in the high,middle and low dose groups of the Qinbai decoction were better than those in the model group, and showed a dose-dependent relationship. Conclusion: The therapeutic effect of Qinbai decoction on ulcerative colitis may be through the expression of IL17, and then down-regulation of the expression of inflammatory factors to improve the pathological changes of ulcerative colitis.
引文
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[10]祝斌,张寒仙,曾今诚,等.美沙拉嗪对DSS诱导小鼠溃疡性结肠炎模型Th1、Th17及Treg细胞亚群的影响[J].中国病理生理杂志,2014,30(12):2219-2225.
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[13]Feng J S,Yang Z,Zhu Y Z,et al.Serum IL-17 and IL-6 increased accompany with TGF-βand IL-13respectively in ulcerative colitis patients[J].Int J lin Exp Med,2014,7(12):5498-5504.
[14]Zheng Z D,Wan X Q,Liu L Y.Serum contents of IL-23 and IL-17 in the patients with ulcerative colitis and the clinical significance[J].Chinese Journal of Cellular and Molecular Immunology,2011,27(2):203-206.
[15]Rafa H,Saoula H,Belkhelfa M,et al.IL-23/IL-17Aaxis correlates with the nitric oxide pathway in inflammatory bowel disease:immunomodulatory effect of retinoic acid[J].J Interferon Cytokine Res,2013,33(7):355-368.
[2]Wang K,Xuan X M,Tong L,et al.The role of Treg Thl7 and calprotectin in ulcerative colitis rat model[J]Sichuan Da Xue Xue Bao Yi Xue Ban,2014,45(6):946-949.
[3]李军祥,陈誩.溃疡性结肠炎中西医结合诊疗共识意见(2017年)[J].中国中西医结合消化杂志,2018,26(2):105-111,120.
[4]肖超,吕照文,付肖冰,等.复方芩柏颗粒剂保留灌肠治疗慢性放射性直肠炎湿热内蕴证30例临床观察[J].中医药导报,2014,20(2):44-46.
[5]王志斌,陈晨,郭一,等.清肠温中方治疗轻中度溃疡性结肠炎的临床研究[J].中国中西医结合杂志,2018,38(1):15-19.
[6]李辉标,陈慕媛,丘振文,等.复方黄柏液治疗溃疡性结肠炎的Meta分析[J].中医药导报,2018,24(7):98-101.
[7]贺荔枝,尹明,王真权.复方芩柏颗粒剂对溃疡性结肠炎大鼠结肠黏膜AQP4、AQP8表达的影响[J].湖南中医药大学学报,2015,35(5):13-16,73.
[8]申睿,刘苗,朱向东,等.中医药治疗溃疡性结肠炎实验研究进展[J].中草药,2018,49(7):1721-1725.
[9]陆胡琳,沈洪,姚宏凤,等.清肠化湿方对小鼠溃疡性结肠炎Th17/Treg平衡的调节作用[J].南京中医药大学报,2014,30(2):130-133.
[10]祝斌,张寒仙,曾今诚,等.美沙拉嗪对DSS诱导小鼠溃疡性结肠炎模型Th1、Th17及Treg细胞亚群的影响[J].中国病理生理杂志,2014,30(12):2219-2225.
[11]孙军刚,刘琦,吴巧凤.针刺对溃疡性结肠炎Th17/Treg免疫平衡的调节作用[J].西部中医药,2015,28(1):129-131.
[12]Ctan C S,Berindan Neagoe I,Cozma V,et al.Contribution of the IL-17/IL-23 axis to the pathogenesis of inflammatory bowel disease[J].World J Gastroenterol,2015,21(19):5823-5830.
[13]Feng J S,Yang Z,Zhu Y Z,et al.Serum IL-17 and IL-6 increased accompany with TGF-βand IL-13respectively in ulcerative colitis patients[J].Int J lin Exp Med,2014,7(12):5498-5504.
[14]Zheng Z D,Wan X Q,Liu L Y.Serum contents of IL-23 and IL-17 in the patients with ulcerative colitis and the clinical significance[J].Chinese Journal of Cellular and Molecular Immunology,2011,27(2):203-206.
[15]Rafa H,Saoula H,Belkhelfa M,et al.IL-23/IL-17Aaxis correlates with the nitric oxide pathway in inflammatory bowel disease:immunomodulatory effect of retinoic acid[J].J Interferon Cytokine Res,2013,33(7):355-368.