杀线虫海洋真菌BH0531发酵液及菌体代谢物对比分析
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摘要
杀线虫海洋枝顶孢霉BH0531是以松材线虫为靶标从渤海水域分离获得的一株海洋真菌。为进一步探明其活性代谢物的性质和类型,实验采用非靶向代谢组学分析,对发酵液及菌体代谢物进行定性检测鉴定测定结果显示,发酵液与菌体共有的代谢物160种,只存在于发酵液中的代谢物87种。其中与乙酰胆碱酯酶活性相关物质有3种;促进植株生长作用相关物质有6种;抗氧化相关物质有6种;抑菌相关物质有3种。从代谢物角度为BH0531菌株的进一步开发利用提供实验依据。
The nematocidal marine-derived Acremonium sp.BH0531 is a marine fungus isolated from the Bohai Seawater with the target of pinewood nematode.In order to further explore the nature and type of active metabolites,the experiment used non-targeted metabolomics analysis to qualitatively detect and determine metabolites from fermentation broth and fungal cell body.The results showed that 160 metabolites shared by fermentation broth and cell body and 87 metabolites present only in the fermentation broth.Among them,there are 3 kinds of active substances related to the activity of acetylcholinesterase;6 kinds of active substances related to plant growth;6 kinds of active substances related to anti-oxidation;3 kinds of active substances antibacterial related.It provides experimental basis for the further development and utilization of BH0531 strains from the perspective of metabolites.
The nematocidal marine-derived Acremonium sp.BH0531 is a marine fungus isolated from the Bohai Seawater with the target of pinewood nematode.In order to further explore the nature and type of active metabolites,the experiment used non-targeted metabolomics analysis to qualitatively detect and determine metabolites from fermentation broth and fungal cell body.The results showed that 160 metabolites shared by fermentation broth and cell body and 87 metabolites present only in the fermentation broth.Among them,there are 3 kinds of active substances related to the activity of acetylcholinesterase;6 kinds of active substances related to plant growth;6 kinds of active substances related to anti-oxidation;3 kinds of active substances antibacterial related.It provides experimental basis for the further development and utilization of BH0531 strains from the perspective of metabolites.
引文
[1]李娜,张金源,苗利华.一株杀线虫活性海洋真菌BH0531的培养特性及种属鉴定[J].中国酿造,2018,37(03):38-41.
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[3]申佩娟,苗利华,李娜.海洋枝顶孢霉BH0531发酵液对根结线虫卵及二龄幼虫的影响[J].中国酿造,2017,36(04):26-29.
[4]孟凡欢,王勇勇,蒙春蕾.渤海湾丝状海洋真菌抑菌活性菌株的调查研究[J].天津师范大学学报(自然科学版),2014,34(04):64-67.
[5]王勇勇,蒙春蕾,蔡爽.抗白色念珠菌海洋真菌的筛选及抑菌活性初探[J].中国酿造,2015,34(02):55-59.
[6]黄敏,刘晓霞,申佩娟.海洋枝顶孢霉BH0531对黄瓜根结线虫防治的微生态效应[J].中国酿造,2016,35(03):52-56.
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[14]金黎明,杨艳,刘万顺.氨基葡萄糖对CCl4所致小鼠肝损伤的改善作用[J].郑州大学学报(医学版),2009,44(01):122-125.
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[17]董杰杰,张晓勇,鲍洁.海洋真菌Aspergillus sp.SCSGAF0076与细菌Bacillus sp.MNMCCE001共培养次生代谢产物[J].微生物学报,2014,54(11):1289-1295.
[2]Meng Q H,Shi X X,Meng F H.Isolation of an Acremonium sp from a screening of 52 seawater fungal isolates and preliminary characterization of its growth conditions and nematicidal activity[J].Biology Letters,2012,34:1847-1850.
[3]申佩娟,苗利华,李娜.海洋枝顶孢霉BH0531发酵液对根结线虫卵及二龄幼虫的影响[J].中国酿造,2017,36(04):26-29.
[4]孟凡欢,王勇勇,蒙春蕾.渤海湾丝状海洋真菌抑菌活性菌株的调查研究[J].天津师范大学学报(自然科学版),2014,34(04):64-67.
[5]王勇勇,蒙春蕾,蔡爽.抗白色念珠菌海洋真菌的筛选及抑菌活性初探[J].中国酿造,2015,34(02):55-59.
[6]黄敏,刘晓霞,申佩娟.海洋枝顶孢霉BH0531对黄瓜根结线虫防治的微生态效应[J].中国酿造,2016,35(03):52-56.
[7]杜海霞,蒙春蕾,王勇勇,等.海洋枝顶孢霉BH0531代谢物对松材线虫形态和酶活的影响[J].中国酿造,2014,33(06):23-26.
[8]蔡爽,申佩娟,刘晓霞.海洋真菌BH0531对黄瓜促生长作用的研究[J].中国酿造,2016,35(03):27-31.
[9]Ding Z S,Tian S P,Zheng X L.Response of reactive oxygen metabolism and quality in mango fruit to exogenous oxalic acid or salicylic acid under low-temperature stress[J].Physiologia Plantarum,2007,130:112-121.
[10]Zhang Y Z,Zhang M L,Yang H Q.Postharvest chitosangsalicylic acid application alleviates chilling injury and preserves cucumber fruit quality during cold storage[J].Food Chemistry,2015,174:558-563.
[11]张军民,石晓峰.天然产物莽草酸的研究进展[J].中国中医药信息杂志,2011,18(02):109-112.
[12]姜骅,唐成康.泛酸研究及其应用[J].四川食品与发酵,2004(01):11-13.
[13]刘术均,刘爱群,何明.外源甜菜碱对低温胁迫下茄子幼苗抗性的影响[J].北方园艺,2018(22):57-61.
[14]金黎明,杨艳,刘万顺.氨基葡萄糖对CCl4所致小鼠肝损伤的改善作用[J].郑州大学学报(医学版),2009,44(01):122-125.
[15]武菲,付玉杰.葫芦巴碱在植物体内生理功能[J].安徽农业科学,2012,40(10):5876-5878+5914.
[16]薛晋红.橙皮素对H2O2诱导心肌细胞氧化损伤的保护作用及其机制研究[D].山西医科大学,2017.
[17]董杰杰,张晓勇,鲍洁.海洋真菌Aspergillus sp.SCSGAF0076与细菌Bacillus sp.MNMCCE001共培养次生代谢产物[J].微生物学报,2014,54(11):1289-1295.