超声波辅助碱法提取紫花苜蓿叶蛋白的工艺研究
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摘要
主要研究在碱法提取紫花苜蓿叶蛋白的前提下,利用超声波辅助提取以缩短蛋白质提取时间及提高提取率。在单因素试验的基础上,通过正交试验得出超声波辅助碱法提取苜蓿叶蛋白的最佳工艺条件:温度为40℃,pH为8.5,超声时间为30 min,此时粗蛋白提取率为51.09%。此法与单纯的碱法提取蛋白比较,大大缩短提取时间,并且提高了苜蓿叶蛋白的提取率。
Under the premise of the alkali extraction of leaf protein from alfalfa,ultrasonic-assisted alkali extraction was studied in order to reduce extraction time and increase the protein extraction rate.On the basis of single-factor tests,the optimal conditions for ultrasonic-assisted alkali extraction are achieved by the orthogonal experimental,namely temperature 40 ℃,pH 8.5,ultrasonic time 30 min.Under these technical conditions,extraction rate of protein was 51.09%.Compared with alkali method extraction,the extraction time of alfalfa leaf protein of ultrasonic-assisted alkali extraction was greatly shortened,and extraction rate was improved.
Under the premise of the alkali extraction of leaf protein from alfalfa,ultrasonic-assisted alkali extraction was studied in order to reduce extraction time and increase the protein extraction rate.On the basis of single-factor tests,the optimal conditions for ultrasonic-assisted alkali extraction are achieved by the orthogonal experimental,namely temperature 40 ℃,pH 8.5,ultrasonic time 30 min.Under these technical conditions,extraction rate of protein was 51.09%.Compared with alkali method extraction,the extraction time of alfalfa leaf protein of ultrasonic-assisted alkali extraction was greatly shortened,and extraction rate was improved.
引文
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[2]SRIVASIAVA R R.Effects of growth stage on the extractability and composition of leaf protein concentrate of some weeds[J].Indian Journal of Agricultural Research,1988,22(2):92-96.
[3]BARANIAK B,KARAS M.Antioxidative properties of chloroplast concentrates obtained by various methods from Lucerne juice[J].Journal of Animal and Feed Sciences,2000,9(2):397-405.
[4]杨春波,谢正军,金征宇.从压块苜蓿草中提取蛋白质的工艺研究[J].食品与机械,2006,22(6):23-25.
[5]崔志芹,王志祥,史美仁.超声波对棉籽蛋白萃取过程的强化[J].中国油脂,2006,31(6):60.
[6]李惠敏,秦新民,覃屏生,等.超声波提取沙田柚花粉管可溶性蛋白的初步研究[J].广西师范大学学报:自然科学版,2004,22(1):82-85.
[7]谢姣.超声波辅助碱法提取红橘种子蛋白质的研究[J].山东食品发酵,2012,2:17-20.
[8]张水华.食品分析[M].中国轻工业出版社,2007,2:159-160.