超声波法优化提取双孢蘑菇废弃菇柄蛋白质
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摘要
为促进双孢蘑菇废弃菇柄的综合利用,增加其经济价值,以双孢蘑菇废弃菇柄为试材,采用超声波提取菇柄中的蛋白质。在单因素试验基础上,选取超声波功率、液固比、pH值为影响因素,以蛋白质提取率为指标,采用正交试验对蛋白质提取进行优化。结果表明,在超声处理时间为1 min时,蛋白质最佳提取工艺参数为超声波功率150 W、液固比35:1 mL/g、pH10.0,在此优化条件下蛋白质提取率可达6.87 g/100 g。与传统的碱法提取相比,超声波法蛋白质提取率约为碱法提取的3.5倍,时间缩短了60倍。超声波提取操作简单、高效、快速、安全,反应过程无副反应发生,是一种安全、经济和简便的提取方法,可用于多种有效物质提取。
To promote the utilization of Agaricus bisporus waste stipe and increase its economic value, protein were extracted from Agaricus bisporus waste stipe by ultrasonic extraction. The effect of ultrasonic power, ratio of liquid to solid, ultrasonic time, pH value on the yield of protein were investigated by single factor experiments, and optimized the parameters by orthogonal experiment use ultrasonic power, ratio of liquid to solid and pH value as factors. The results showed that as the ultrasonic time was 1 min, the parameters optimized by orthogonal experiment analysis were as follows: ultrasonic power 150 W, liquid-solid ratio 35:1 mL/g, pH value 10.0. Under these optimum conditions, the yield of protein extract from Agaricus bisporus waste stipe can be up to 6.87 g/100 g, which was 3.5 times higher than traditional alkali extraction, and shorten 60 times than traditional alkali extraction in time. As a simple, effective, fast, safe and no adverse reaction, ultrasonic extraction is effective extraction method, and can be used to extract all functional components.
To promote the utilization of Agaricus bisporus waste stipe and increase its economic value, protein were extracted from Agaricus bisporus waste stipe by ultrasonic extraction. The effect of ultrasonic power, ratio of liquid to solid, ultrasonic time, pH value on the yield of protein were investigated by single factor experiments, and optimized the parameters by orthogonal experiment use ultrasonic power, ratio of liquid to solid and pH value as factors. The results showed that as the ultrasonic time was 1 min, the parameters optimized by orthogonal experiment analysis were as follows: ultrasonic power 150 W, liquid-solid ratio 35:1 mL/g, pH value 10.0. Under these optimum conditions, the yield of protein extract from Agaricus bisporus waste stipe can be up to 6.87 g/100 g, which was 3.5 times higher than traditional alkali extraction, and shorten 60 times than traditional alkali extraction in time. As a simple, effective, fast, safe and no adverse reaction, ultrasonic extraction is effective extraction method, and can be used to extract all functional components.
引文
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[3]徐伟.双孢蘑菇菇柄中多糖、核酸等成分的提取利用[J].科技创新导报,2012,(9):237
[4]林忠宁,陈敏健,刘明香,等.双孢蘑菇菇脚氨基酸含量的测定及营养评价[J].氨基酸和生物资源,2012,33(4):20-23
[5]刘香萍,李国良,王凤军.酸热法提取苜蓿叶蛋白初探[J].吉林畜牧兽医,2007,(1):10-11
[6]杨远帆,李杏,陈申如,等.弹性蛋白酶提取牛蛙皮胶原蛋白的工艺优化[J].中国食品学报,2013,13(10):66-72
[7]刘庆,李超,耿中华,等.碱提酸沉法制备杏鲍菇蛋白质的工艺优化[J].食品工业,2013,(10):145-148
[8]张喜峰,孙亚莉,李鑫文,等.双水相萃取分离葡萄酒发酵下脚料中葡萄籽蛋白质[J].云南大学学报(自然科学版),2013,(6):824-832
[9]杨秋慧子,张志宽,陈雨泰,等.反胶团萃取分离红豆蛋白质[J].食品工业科技,2013,34(19):252-256
[10]王静,韩涛,李丽萍.超声波的生物效应及其在食品工业中的应用[J].北京农学院学报,2006,(1):67-75
[11]朱新鹏.超声波在天然产物活性成分提取中的应用[J].保鲜与加工,2012,(2):43-45
[12]曹向宇,刘剑利,齐峰,等.超声波联合酶法提取鸡腿菇菌丝体多糖及纯化[J].食品科学,2013,34(16):6-10
[13]Bradford M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J].Analytical Biochemistry,1976,72(1-2):248-254
[14]李顺峰.真姬菇子实体多糖的提取、纯化及抗氧化性研究[D].杨凌:西北农林科技大学硕士学位论文,2008
[15]黄群,杨万根,余佶,等.超声波辅助碱法提取杜仲籽粕可溶性膳食纤维的工艺优化[J].食品科学,2013,34(22):70-74
[16]张相年,赵树进,李超.蛋白分离技术的应用和进展[J].中国药业,2006,15(2):72-73
[17]Ansharullah,Hourigan J A,Chesterman C F.Application of Carbohydrases in Extracting Protein from Rice Bran[J].Journal of the Science of Food and Agriculture,1997,74(2):141-146
[18]陆晨,张士康,朱科学,等.碱提酸沉法提取茶叶蛋白质的研究[J].现代食品科技,2011,27(6):673-677
[19]Shih F F,Daigle K.Use of enzymes for the separation of protein from rice flour[J].Cereal Chemistry,1997,74(4):437-441