植物叶蛋白提取方法及研究进展
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摘要
植物叶蛋白营养全面,具有很高的食用价值和药用价值,被广泛应用于食品、饲料和医疗等领域中,是一种极具开发价值的新型蛋白质资源,具有广阔的开发前景。提取叶蛋白的常用方法有加热法、酸碱法、盐析法和有机溶剂法等,对这些提取方法及国内外相关研究进行了综述,以期使这种来源广泛易得的低值原料得以充分提取并实现高值利用。
The nutrients of plant leaf protein are comprehensive. Leaf protein has high edible value and medicinal value, which can be applied in food, feed, medical and other fields. It is a new protein resources, which has great potential development value. Methods suitable for leaf protein extraction include heating method, heating method combined with acid and alkali treatment, salting-out method and organic solvent method, and so on. This paper summarized leaf protein extraction methods and the related researches both domestic and overseas with an expection that this sort of low-cost material could be fully extracted with proper method and realize its high-value use.
The nutrients of plant leaf protein are comprehensive. Leaf protein has high edible value and medicinal value, which can be applied in food, feed, medical and other fields. It is a new protein resources, which has great potential development value. Methods suitable for leaf protein extraction include heating method, heating method combined with acid and alkali treatment, salting-out method and organic solvent method, and so on. This paper summarized leaf protein extraction methods and the related researches both domestic and overseas with an expection that this sort of low-cost material could be fully extracted with proper method and realize its high-value use.
引文
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[9]Bu-Abbas A,Clifford M N,Walker R,et al.Selective induction of rat hepatic CYP1 and CYP4 proteins and of peroxisomal proliferation by green tea[J].Carcinogenesis,1994,15(11):2575-2579.
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[18]黄威.南瓜叶蛋白加工方法及其制品的安全性评价研究[D].重庆:西南大学,2010.
[19]贺新强,宋葆华,倪陈凯,等.植物叶蛋白提取方法的比较[J].植物资源与环境,1999(2):64-65.
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[25]李圆圆.茶渣蛋白的酶法提取及功能性质研究[D].无锡:江南大学,2013.
[26]江洪波,雷挺.桑叶叶蛋白提取工艺的研究[J].农产品加工:学刊,2007(12):19-21.
[27]舒友琴,梁丽琴,扶庆权,等.菠菜叶蛋白的提取研究[J].食品科学,2005(10):124-127.
[28]王桃云,王金虎.桑叶叶蛋白提取工艺的研究[J].食品研究与开发,2006(2):79-81.
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[30]王丽,苏印泉,张强,等.光叶楮叶蛋白提取工艺优化和氨基酸分析研究[J].西北林学院学报,2012(2):137-142.
[31]朱宇旌,张勇,李玉杰,等.苜蓿干草提取叶蛋白最佳工艺的研究[J].中国饲料,2006(2):33-36.
[32]张垚,白志明,董宽虎,等.苜蓿鲜草提取叶蛋白最适条件的研究[J].中国草地学报,2007(6):69-72,80.
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[34]熊春梅,郭爱伟,陈粉粉,等.三叶草叶蛋白提取工艺优化研究[J].中国畜牧兽医,2011(12):61-63.
[35]刘晓峰,达哇卓玛,杨国柱.“青大一号”紫花苜蓿叶蛋白提取研究[J].安徽农业科学,2013,41(13):5727-5730.
[36]李凤玲,何金环.聚合草鲜草叶蛋白提取条件研究[J].中国草地学报,2009(2):76-80.
[37]董双涛,李宝霞,霍乃蕊.TCA-丙酮沉淀法提取聚合草叶蛋白的研究[J].世界中西医结合杂志,2011(9):766-767,828.
[38]Vergara-Barberán M,Lerma-García M J,Herrero-Martínez J M,et al.Use of an enzyme-assisted method to improve protein extraction from olive leaves[J].Food Chemistry,2015,169:28-33.
[39]林燕如,蔡楷钰.菜用黄麻叶蛋白最佳提取工艺研究[J].北方园艺,2013(12):144-147.
[40]饶国华,赵谋明,林伟锋,等.低次烟叶蛋白质提取工艺研究[J].西北农林科技大学学报:自然科学版,2005(11):67-72.
[41]程道梅,韩珍琼.甘薯叶蛋白提取方法对提取率及营养成分的影响研究[J].食品科技,2010(11):208-210.
[42]吕巧枝,木泰华,孙艳丽.甘薯叶可溶性蛋白提取工艺研究[J].食品研究与开发,2007(3):18-22.
[43]刘杰,贾士儒,钟成,等.柠条叶蛋白碱性提取的响应面分析法优化[J].天津科技大学学报,2011(1):1-4.
[44]曲敏,马永强,杨大鹏,等.不同方法提取苜蓿叶蛋白效果的比较及表征[J].食品科学,2012(14):91-95.
[45]曾凡枝,田丽萍,薛琳,等.直接发酵法提取苜蓿叶蛋白[J].新疆农业科学,2008(5):890-893.
[46]Ansharullah,James A H,Colin F C.Application of carbohydrases in protein from rice bran[J].Science of Food and Agriculture,1997,74:141-146.
[47]汪建旭,冯炜弘,杨道兰,等.花椰菜废弃茎叶中叶蛋白提取工艺的研究[J].食品工业科技,2013(22):253-256,284.
[48]梁丽琴,魏学智,段江燕,等.超声波辅助提取扁核木叶蛋白的工艺优化[J].中国粮油学报,2012(12):96-100.
[2]Sajid L,Farooq A,Abdullah I H,et al.Aqueous enzymatic process for oil and protein extraction from moringa oleifera seed[J].Lipid Science and Technology,2011,113:1012-1018.
[3]Ezeagu I E,Gowda L R.Protein extractability,fractionation and amino acid composition of some leguminous seeds found in nigeria[J].Food Biochemistry,2006,30:1-11.
[4]Olvera-Novoa M A,Campos S G,Sabido M G,et al.The use of alfalfa leaf protein concentrates as a protein source in diets for tilapia(Oreochromis mossambicus)[J].Aquaculture,1990,90:291-302.
[5]Anelli G,Fiorentini R,Massignan L,et al.The poly-protein process:A new method for obtaining leaf protein concentrates[J].Food Science,2006,42:1401.
[6]Zhang B,Xie C,Wei Y,et al.Purification and characterisation of an antifungal protein,MCha-Pr,from the intercellular fluid of bitter gourd(Momordica charantia)leaves[J].Protein Expr Purif,2015,107:43-49.
[7]Vyas S,Collin S M,Bertin E,et al.Leaf concentrate as an alternative to iron and folic acid supplements for anaemic adolescent girls:a randomised controlled trial in India[J].Public Health Nutr,2012,13(3):418-423.
[8]Ng T B.Antifungal proteins and peptides of leguminous and non-leguminous origins[J].Peptides,2004,25:1215-1222.
[9]Bu-Abbas A,Clifford M N,Walker R,et al.Selective induction of rat hepatic CYP1 and CYP4 proteins and of peroxisomal proliferation by green tea[J].Carcinogenesis,1994,15(11):2575-2579.
[10]Baraniak B,Karas M.Antioxidative properties of chloroplast concentrates obtained by various methods from luceme juice[J].Animal and Feed Sciences,2000,9:397-405.
[11]Julius K,Tangka.Analysis of the thermal energy requirements for the extraction of leaf protein concentrate from some green plants[J].Biosystems Engineering,2003,86:473-479.
[12]Rathore M.Leaf protein concentrate as food supplement from arid zone plants[J].J Diet Suppl,2010,7(2):97-103.
[13]Wu W,Sun Y.Dietary safety evaluation of water hyacinth leaf protein concentrate[J].Hum Exp Toxicol,2011,30(10):1514-1520.
[14]肖海峻,孟利前,杨新建,等.苜蓿叶蛋白提取工艺参数优化试验[J].食品研究与开发,2010(11):39-43.
[15]薛琳,刘青广,唐慧,等.苜蓿叶蛋白提取工艺参数优化试验研究[J].石河子大学学报,2005(23):152-154.
[16]董欣炜.紫花苜蓿叶蛋白提取工艺研究[D].重庆:西南大学,2006.
[17]吕富,茅玲燕,季涛,等.水葫芦叶蛋白提取工艺初步研究[J].盐城工学院学报:自然科学版,2013(1):10-13.
[18]黄威.南瓜叶蛋白加工方法及其制品的安全性评价研究[D].重庆:西南大学,2010.
[19]贺新强,宋葆华,倪陈凯,等.植物叶蛋白提取方法的比较[J].植物资源与环境,1999(2):64-65.
[20]吕宗友,赵国琦,苏衍菁,等.白三叶叶蛋白提取及纯化工艺[J].草业科学,2011(11):2052-2056.
[21]唐世敏,姜东燕.不同处理对苜蓿叶蛋白提取的影响[J].安徽农业科学,2012,40(36):17624-17625.
[22]杨超英,刘艳,薛正莲,等.黑麦草叶蛋白提取工艺研究[J].中国农学通报,2007(5):14-17.'
[23]董双涛,李宝霞,霍乃蕊.聚合草叶蛋白的酸化加热法提取工艺研究[J].山西中医学院学报,2012(2):45-47.
[24]吴万灵,韩鲁佳,王唯涌.苜蓿叶蛋白提取工艺参数优化试验研究[J].中国农业大学学报,2006(5):65-69.
[25]李圆圆.茶渣蛋白的酶法提取及功能性质研究[D].无锡:江南大学,2013.
[26]江洪波,雷挺.桑叶叶蛋白提取工艺的研究[J].农产品加工:学刊,2007(12):19-21.
[27]舒友琴,梁丽琴,扶庆权,等.菠菜叶蛋白的提取研究[J].食品科学,2005(10):124-127.
[28]王桃云,王金虎.桑叶叶蛋白提取工艺的研究[J].食品研究与开发,2006(2):79-81.
[29]衣丹,刘发义,臧家业,等.响应面法优化碱蓬叶蛋白提取工艺研究[J].食品工业,2011(10):52-54.
[30]王丽,苏印泉,张强,等.光叶楮叶蛋白提取工艺优化和氨基酸分析研究[J].西北林学院学报,2012(2):137-142.
[31]朱宇旌,张勇,李玉杰,等.苜蓿干草提取叶蛋白最佳工艺的研究[J].中国饲料,2006(2):33-36.
[32]张垚,白志明,董宽虎,等.苜蓿鲜草提取叶蛋白最适条件的研究[J].中国草地学报,2007(6):69-72,80.
[33]柳斌,席亚丽,穆峰海,等.不同处理条件对苜蓿叶蛋白凝聚效果的研究[J].草业科学,2010(1):114-118.
[34]熊春梅,郭爱伟,陈粉粉,等.三叶草叶蛋白提取工艺优化研究[J].中国畜牧兽医,2011(12):61-63.
[35]刘晓峰,达哇卓玛,杨国柱.“青大一号”紫花苜蓿叶蛋白提取研究[J].安徽农业科学,2013,41(13):5727-5730.
[36]李凤玲,何金环.聚合草鲜草叶蛋白提取条件研究[J].中国草地学报,2009(2):76-80.
[37]董双涛,李宝霞,霍乃蕊.TCA-丙酮沉淀法提取聚合草叶蛋白的研究[J].世界中西医结合杂志,2011(9):766-767,828.
[38]Vergara-Barberán M,Lerma-García M J,Herrero-Martínez J M,et al.Use of an enzyme-assisted method to improve protein extraction from olive leaves[J].Food Chemistry,2015,169:28-33.
[39]林燕如,蔡楷钰.菜用黄麻叶蛋白最佳提取工艺研究[J].北方园艺,2013(12):144-147.
[40]饶国华,赵谋明,林伟锋,等.低次烟叶蛋白质提取工艺研究[J].西北农林科技大学学报:自然科学版,2005(11):67-72.
[41]程道梅,韩珍琼.甘薯叶蛋白提取方法对提取率及营养成分的影响研究[J].食品科技,2010(11):208-210.
[42]吕巧枝,木泰华,孙艳丽.甘薯叶可溶性蛋白提取工艺研究[J].食品研究与开发,2007(3):18-22.
[43]刘杰,贾士儒,钟成,等.柠条叶蛋白碱性提取的响应面分析法优化[J].天津科技大学学报,2011(1):1-4.
[44]曲敏,马永强,杨大鹏,等.不同方法提取苜蓿叶蛋白效果的比较及表征[J].食品科学,2012(14):91-95.
[45]曾凡枝,田丽萍,薛琳,等.直接发酵法提取苜蓿叶蛋白[J].新疆农业科学,2008(5):890-893.
[46]Ansharullah,James A H,Colin F C.Application of carbohydrases in protein from rice bran[J].Science of Food and Agriculture,1997,74:141-146.
[47]汪建旭,冯炜弘,杨道兰,等.花椰菜废弃茎叶中叶蛋白提取工艺的研究[J].食品工业科技,2013(22):253-256,284.
[48]梁丽琴,魏学智,段江燕,等.超声波辅助提取扁核木叶蛋白的工艺优化[J].中国粮油学报,2012(12):96-100.