西藏山羊免疫小反刍兽疫活疫苗的临床免疫效果试验
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摘要
本研究旨在评估由西藏自治区兽医生物药品制造厂生产的小反刍兽疫活疫苗(Clone9株)的临床免疫效果,为西藏山羊的免疫接种提供一定的理论依据。本研究选用免疫接种、体温监测和抗体检测等方法,对该疫苗的免疫安全性和免疫抗体水平情况进行了临床免疫测试和评估。结果表明免疫100头份活疫苗的山羊无免疫副作用,免疫14 d内均未出现临床症状,并且山羊体温均未出现异常;免疫0.1头份活疫苗的山羊精神状态良好,免疫21 d后山羊的PPR抗体阳性率达到100%。结果说明小反刍兽疫活疫苗(Clone9株)的免疫安全性良好,并且具有良好的免疫原性,可产生极好的临床免疫效果。
This experiment evaluated the clinical immune effect of Peste des petis ruminant live vaccine(Clone9 strain) produced by Tibet Veterinary Biological Pharmaceutical Factory to provide a theoretical basis for immunization of Tibetan goats. In this study, clinical immunization tests and evaluation were conducted on the immune safety and immune antibody levels of the vaccine by means of immunization, body temperature monitoring and antibody detection. The results showed that the goats immunized with 100 live vaccines had no immune side effects, and no clinical symptoms were observed within 14 days of immunization, and no abnormalities were observed in the body temperature of goats. The goats immunized with 0.1 live vaccine were in good mental state, and the positive rate of PPR antibody in goats reached 100% after 21 days of immunization. These results indicated that the Peste des petis ruminant live vaccine(Clone9 strain)had good immunological safety and good immunogenicity, which produced excellent clinical immune effect.
This experiment evaluated the clinical immune effect of Peste des petis ruminant live vaccine(Clone9 strain) produced by Tibet Veterinary Biological Pharmaceutical Factory to provide a theoretical basis for immunization of Tibetan goats. In this study, clinical immunization tests and evaluation were conducted on the immune safety and immune antibody levels of the vaccine by means of immunization, body temperature monitoring and antibody detection. The results showed that the goats immunized with 100 live vaccines had no immune side effects, and no clinical symptoms were observed within 14 days of immunization, and no abnormalities were observed in the body temperature of goats. The goats immunized with 0.1 live vaccine were in good mental state, and the positive rate of PPR antibody in goats reached 100% after 21 days of immunization. These results indicated that the Peste des petis ruminant live vaccine(Clone9 strain)had good immunological safety and good immunogenicity, which produced excellent clinical immune effect.
引文
[1]Baron M D,Diallo A,Lancelot R,etc.Peste des Petits Ruminants Virus[J].Adv Virus Res,2016,95:1-42.
[2]信爱国,杨仁标,向文彬.小反刍兽疫研究进展[J].中国预防兽医学报,2003,(02):73-76.
[3]Parida S,Muniraju M,Mahapatra M,etc.Peste des petits ruminants[J].Vet Microbiol,2015,181(1-2):90-106.
[4]Kumar N,Maherchandani S,Kashyap SK,etc.Peste des petits ruminants virus infection of small ruminants:a comprehensive review[J].Viruses,2014,6(6):2287-2327.
[5]Naeem A,Abu Elzein EM,Afaleq AI.Epizootiological aspects of peste des petits ruminants and rinderpest in sheep and goats in Saudi Arabia[J].Rev Sci Tech,2000,19(3):855-858.
[6]Singh RP,Bandyopadhyay SK.Peste des petits ruminants vaccine and vaccination in India:sharing experience with disease endemic countries[J].Virusdisease,2015,26(4):215-224.
[7]Abubakar M,Irfan M,Manzoor S.Peste des petits ruminants in Pakistan;past,present and future perspectives[J].J Anim Sci Technol,2015,57:32.
[8]Wang Z,Bao J,Wu X,etc.Peste des petits ruminants virus in Tibet,China[J].Emerg Infect Dis,2009,15(2):299-301.
[9]王志亮,包静月,吴晓东等.我国首例小反刍兽疫诊断报告[J].中国动物检疫,2007(08):24-26.
[10]吴锦艳,尚佑军,田宏等.2007-2014年国内外小反刍兽疫流行现状及分析[J].中国兽医学报,2016,36(04):687-693.
[11]Wu X,Li L,Li J,etc.Peste des Petits Ruminants Viruses Re-emerging in China,2013-2014[J].Transbound Emerg Dis,2016,63(5):441-446.
[12]周海宁,王进香,艾文等.宁夏首例小反刍兽疫疫情诊断报告[J].黑龙江畜牧兽医,2016(04):84-85.
[13]王乐元,次真,吴国珍等.中国西藏小反刍兽疫的发生状况与防控[J].畜牧兽医学报,2011,42(05):717-720.
[14]Balamurugan V,Hemadri D,Gajendragad MR,etc.Diagnosis and control of peste des petits ruminants:a comprehensive review[J].Virusdisease,2014,25(1):39-56.
[15]Sen A,Saravanan P,Balamurugan V,etc.Vaccines against peste des petits ruminants virus[J].Expert Rev Vaccines,2010,9(7):785-796.
[16]郭昌明,王新平.小反刍兽疫诊断与防控的现状及展望[J].黑龙江畜牧兽医,2018(05):68-72.
[17]周伟伟,陈未,陈飞.小反刍兽疫研究进展与疫情动态[J].黑龙江畜牧兽医,2015(04):82-83.
[18]王善辉,高三阳,沈志强.小反刍兽疫疫苗的研究进展[J].黑龙江畜牧兽医,2011(21):28-30.
[2]信爱国,杨仁标,向文彬.小反刍兽疫研究进展[J].中国预防兽医学报,2003,(02):73-76.
[3]Parida S,Muniraju M,Mahapatra M,etc.Peste des petits ruminants[J].Vet Microbiol,2015,181(1-2):90-106.
[4]Kumar N,Maherchandani S,Kashyap SK,etc.Peste des petits ruminants virus infection of small ruminants:a comprehensive review[J].Viruses,2014,6(6):2287-2327.
[5]Naeem A,Abu Elzein EM,Afaleq AI.Epizootiological aspects of peste des petits ruminants and rinderpest in sheep and goats in Saudi Arabia[J].Rev Sci Tech,2000,19(3):855-858.
[6]Singh RP,Bandyopadhyay SK.Peste des petits ruminants vaccine and vaccination in India:sharing experience with disease endemic countries[J].Virusdisease,2015,26(4):215-224.
[7]Abubakar M,Irfan M,Manzoor S.Peste des petits ruminants in Pakistan;past,present and future perspectives[J].J Anim Sci Technol,2015,57:32.
[8]Wang Z,Bao J,Wu X,etc.Peste des petits ruminants virus in Tibet,China[J].Emerg Infect Dis,2009,15(2):299-301.
[9]王志亮,包静月,吴晓东等.我国首例小反刍兽疫诊断报告[J].中国动物检疫,2007(08):24-26.
[10]吴锦艳,尚佑军,田宏等.2007-2014年国内外小反刍兽疫流行现状及分析[J].中国兽医学报,2016,36(04):687-693.
[11]Wu X,Li L,Li J,etc.Peste des Petits Ruminants Viruses Re-emerging in China,2013-2014[J].Transbound Emerg Dis,2016,63(5):441-446.
[12]周海宁,王进香,艾文等.宁夏首例小反刍兽疫疫情诊断报告[J].黑龙江畜牧兽医,2016(04):84-85.
[13]王乐元,次真,吴国珍等.中国西藏小反刍兽疫的发生状况与防控[J].畜牧兽医学报,2011,42(05):717-720.
[14]Balamurugan V,Hemadri D,Gajendragad MR,etc.Diagnosis and control of peste des petits ruminants:a comprehensive review[J].Virusdisease,2014,25(1):39-56.
[15]Sen A,Saravanan P,Balamurugan V,etc.Vaccines against peste des petits ruminants virus[J].Expert Rev Vaccines,2010,9(7):785-796.
[16]郭昌明,王新平.小反刍兽疫诊断与防控的现状及展望[J].黑龙江畜牧兽医,2018(05):68-72.
[17]周伟伟,陈未,陈飞.小反刍兽疫研究进展与疫情动态[J].黑龙江畜牧兽医,2015(04):82-83.
[18]王善辉,高三阳,沈志强.小反刍兽疫疫苗的研究进展[J].黑龙江畜牧兽医,2011(21):28-30.