若尔盖县牦牛轮状病毒和冠状病毒感染的血清学监测
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摘要
本研究旨在了解四川省若尔盖县牦牛轮状病毒(BRV)和冠状病毒(BCoV)的感染情况。采用酶联免疫吸附试验(ELISA)对276份牦牛血清BRV、BCoV抗体进行检测,结果显示:BRV阳性率为99.2%,BCoV阳性率为97.82%,混合感染率为97.1%。表明若尔盖县牦牛的BRV和BCoV感染非常普遍,未来应加强防控。
The aim of this study was to understand the infection status of yak rotavirus(BRV) and coronavirus(BCoV) in Zoige County,Sichuan Province. 276 yak serum BRV and BCoV antibodies were detected by enzyme-linked immunosorbent assay(ELISA). The results showed that the positive rates of BRV and BCoV were 99.2% and 97.82% respectively,and the mixed infection rate was 97.1%. It indicated that the BRV and BCoV infections of the yak in Zoige County were very popular,prevention and control should be strengthened in the future.
The aim of this study was to understand the infection status of yak rotavirus(BRV) and coronavirus(BCoV) in Zoige County,Sichuan Province. 276 yak serum BRV and BCoV antibodies were detected by enzyme-linked immunosorbent assay(ELISA). The results showed that the positive rates of BRV and BCoV were 99.2% and 97.82% respectively,and the mixed infection rate was 97.1%. It indicated that the BRV and BCoV infections of the yak in Zoige County were very popular,prevention and control should be strengthened in the future.
引文
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[2]郭斌,王珊,张菡,等.若尔盖湿地天然牧草生育期变化特征及其对气候变化的响应[J].高原山地气象研究,2018(2):49-57.
[3]何美琳,张焕容,王永,等.川西北牦牛3种病毒性腹泻病血清学调查[J].中国畜牧兽医,2014,41(3):248-251.
[4]常继涛,崔久辉,李建军,等.我国部分地区牛轮状病毒的病原学调查及一株G10P[11]型牛轮状病毒的分离鉴定[J].中国预防兽医学报,2008,30(10):755-759.
[5]张敏,王清江,刘登薇,等.红原牦牛轮状病毒的研究[J].四川畜牧兽医,1986,13(3):2-4.
[6]周芳,岳华,张斌,等.牦牛轮状病毒VP6基因序列分析及RT-PCR检测方法的建立与应用[J].畜牧兽医学报,2016,47(7):1465-1473.
[7]李凡,岳华,邱莞思,等.基于恒温隔绝式荧光PCR技术现场检测牛轮状病毒方法的建立及应用[J].畜牧兽医学报,2017,48(6):1092-1098.
[8] Hasoksuz M,Lathrop S L,Gadfield K L,et al. Isolation of bovine respiratory coronaviruses from feedlot cattle and comparison of their biological and antigenic properties with bovine enteric coronaviruses[J].American Journal of Veterinary Research,1999,60(10):1227-1233.
[9]姚火春,杜念兴,徐为燕,等.牛冠状病毒的血清流行病学调查[J].南京农业大学学报,1990(2):117-121.
[10]石琴,袁立岗,蒲敬伟,等.天山牦牛病毒性腹泻等传染病的血清学监测与调查[J].畜牧与兽医,2015,47(6):166-167.