超声波辅助提取欧李仁中苦杏仁苷的工艺优化
|
摘要
以欧李种仁为原料,采用超声辅助乙醇提取欧李种仁中苦杏仁苷,使用高效液相色谱仪进行检测,以苦杏仁苷得率作为检测指标,以乙醇浓度、液料比、提取温度、超声时间和超声功率为考察因素进行单因素试验,在此基础上结合二水平试验和响应面法对超声提取工艺进行优化,最终确定提取欧李种仁中苦杏仁苷的最佳工艺条件为液料比13:1(mL/g),乙醇浓度79%,提取温度70℃,超声时间40min,超声功率192W,该条件下苦杏仁苷得率可达6.01%。
In this study,the seeds of Prunus humilis were used to extract amygdalin from the kernels by using ultrasound-assisted ethanol,and HPLC was used for detection.The amygdalin extraction rate was used as the detection index,and the ethanol concentration and the liquid-to-material ratio,extraction temperature,ultrasonic time and ultrasonic power were tested by single factor test,and then the relationships between each factor and extraction rate were analyzed.Thereafter,based on the two-level,five-factor experiment and response surface method, ultrasonic extraction process were optimized to obtain the extraction of Eucalyptus seeds.The optimum conditions of amygdalin included liquid to material ratio 13:1(mL/g),ethanol concentration 79%,extraction at 70 ℃,ultrasonic for40 min,and ultrasonic power 192 W,and the final yield was 6.01%.
In this study,the seeds of Prunus humilis were used to extract amygdalin from the kernels by using ultrasound-assisted ethanol,and HPLC was used for detection.The amygdalin extraction rate was used as the detection index,and the ethanol concentration and the liquid-to-material ratio,extraction temperature,ultrasonic time and ultrasonic power were tested by single factor test,and then the relationships between each factor and extraction rate were analyzed.Thereafter,based on the two-level,five-factor experiment and response surface method, ultrasonic extraction process were optimized to obtain the extraction of Eucalyptus seeds.The optimum conditions of amygdalin included liquid to material ratio 13:1(mL/g),ethanol concentration 79%,extraction at 70 ℃,ultrasonic for40 min,and ultrasonic power 192 W,and the final yield was 6.01%.
引文
[1]温琢,高润宏.欧李的研究进展[J].林副产品,2016(12):51-53.
[2]张美莉,邓秋才,杨海霞,等.内蒙古欧李果肉和果仁中营养成分分析[J].氨基酸和生物资源,2007,29(4):18-20.
[3]冯媛媛.欧李营养成分分析及低糖欧李果脯的开发[D].保定:河北农业大学,2015:8-21.
[4]白东海.欧李果实类黄酮物质提取、组分鉴定及抗氧化能力研究[D].太谷:山西农业大学,2015:19-52.
[5]叶丽琴.欧李果肉芳香物质构成特点、种质和地域差异及动态变化的研究[D].北京:北京中医药大学,2017:7-14.
[6]田金强,兰彦平,朱克瑞,等.欧李仁综合利用关键技术研究[J].中国油脂,2012,37(2):65-69.
[7]李秀珍,李学强,卢素丽.微波辅助索氏提取法提取欧李仁油的工艺参数优化[J].生物学通报,2013,48(6):47-49.
[8]高友君,任清.人工栽培欧李仁油的提取、成分分析及抗氧化性[J].食品科学,2015,36(4):80-85.
[9]张玲.欧李仁多肽的制备及其功能活性的研究[D].太谷:山西农业大学,2014:37-42.
[10]张伟,林彤,江英桥.RRLC法同时测定橘红痰咳颗粒中苦杏仁苷和柚皮苷[J].中成药,2012,34(5):865-868.
[11]ZHOU Cun-shan,QIAN Li-chun,MA Hai-le,et al.Enhancement of amygdalin activated withβ-D-glucosidase on HepG2cells proliferation and apoptosis[J].Carbohydrate Polymers,2012,90(1):516-523.
[12]孙卓琳,刘京伟,匡长春.苦杏仁苷抑制人肺癌NCI-H1299细胞体外侵袭的机制[J].中国药科大学学报,2016,47(4):479-482.
[13]MAKAREVIC J,TSAUR I,JUENGEL E,et al.Amygdalin delays cell cycle progression and blocks growth of prostate cancer cells in vitro[J].Life Sciences,2016,147:137-142.
[14]郑巧玲,郭义曹,孙杰,等.苦杏仁苷促进人血T淋巴细胞早熟凝集染色体增殖的实验研究[J].环境与职业医学,2009,26(6):572-574.
[15]郭君其.苦杏仁甙抑制成人肾脏成纤维细胞的增殖[J].中国组织工程研究与临床康复,2008,12(18):3 575-3 578.
[16]DENG Jia-gang,LI Chun-yang,WANG Hai-lian,et al.Amygdalin mediates relieved atherosclerosis in apolipoprotein E deficient mice through the induction of regulatory T cells[J].Biochemical and Biophysical Research Communications,2011,411(3):523-529.
[17]ZHANG Xiang-qun,HU Jian-gong,ZHUO Yu-zhen,et al.Amygdalin improves microcirculatory disturbance and attenuates pancreatic fibrosis by regulating the expression of endothelin-1and calcitonin gene-related peptide in rats[J].Journal of the Chinese Medical Association,2018,81(5):437-443.
[18]赵静,段杨峰,孔繁渊,等.超声波辅助提取枇杷核苦杏仁苷工艺优化[J].食品科学,2011,32(8):37-42.
[19]邬晓勇,孙雁霞,何钢,等.欧李种仁中苦杏仁苷的提取及其抗氧化活性[J].湖北农业科学,2013,52(19):4 764-4 767.
[20]刘进波,肖可,张天其,等.HPLC对比不同炮制方法对苦杏仁药材含量的影响[J].内蒙古中医药,2014(20):78.
[21]谢朝晖,李鑫,李文博,等.超声波法提取山杏仁中苦杏仁苷的单因素试验研究[J].河南城建学院学报,2012,21(2):31-37.
[22]YUE Kai, YE Meng, ZHOU Zu-ji,et al.The genus Cordyceps:a chemical and pharmacological review[J].Journal of Pharmacy&Pharmacology,2013,65(4):474-493.
[23]王涛,夏其乐,陆胜民.超声波提取杨梅疏果核仁中苦杏仁苷工艺研究[J].浙江农业学报,2014,26(3):779-785.
[24]马杰.桃仁苦杏仁苷的提取及其抑菌活性研究[J].食品工业,2015,36(5):116-120.
[25]范贤哲,李广凯,蒋艳杰,等.苦杏仁中苦杏仁苷提取工艺研究[J].广州化工,2018,46(10):81-84.
[2]张美莉,邓秋才,杨海霞,等.内蒙古欧李果肉和果仁中营养成分分析[J].氨基酸和生物资源,2007,29(4):18-20.
[3]冯媛媛.欧李营养成分分析及低糖欧李果脯的开发[D].保定:河北农业大学,2015:8-21.
[4]白东海.欧李果实类黄酮物质提取、组分鉴定及抗氧化能力研究[D].太谷:山西农业大学,2015:19-52.
[5]叶丽琴.欧李果肉芳香物质构成特点、种质和地域差异及动态变化的研究[D].北京:北京中医药大学,2017:7-14.
[6]田金强,兰彦平,朱克瑞,等.欧李仁综合利用关键技术研究[J].中国油脂,2012,37(2):65-69.
[7]李秀珍,李学强,卢素丽.微波辅助索氏提取法提取欧李仁油的工艺参数优化[J].生物学通报,2013,48(6):47-49.
[8]高友君,任清.人工栽培欧李仁油的提取、成分分析及抗氧化性[J].食品科学,2015,36(4):80-85.
[9]张玲.欧李仁多肽的制备及其功能活性的研究[D].太谷:山西农业大学,2014:37-42.
[10]张伟,林彤,江英桥.RRLC法同时测定橘红痰咳颗粒中苦杏仁苷和柚皮苷[J].中成药,2012,34(5):865-868.
[11]ZHOU Cun-shan,QIAN Li-chun,MA Hai-le,et al.Enhancement of amygdalin activated withβ-D-glucosidase on HepG2cells proliferation and apoptosis[J].Carbohydrate Polymers,2012,90(1):516-523.
[12]孙卓琳,刘京伟,匡长春.苦杏仁苷抑制人肺癌NCI-H1299细胞体外侵袭的机制[J].中国药科大学学报,2016,47(4):479-482.
[13]MAKAREVIC J,TSAUR I,JUENGEL E,et al.Amygdalin delays cell cycle progression and blocks growth of prostate cancer cells in vitro[J].Life Sciences,2016,147:137-142.
[14]郑巧玲,郭义曹,孙杰,等.苦杏仁苷促进人血T淋巴细胞早熟凝集染色体增殖的实验研究[J].环境与职业医学,2009,26(6):572-574.
[15]郭君其.苦杏仁甙抑制成人肾脏成纤维细胞的增殖[J].中国组织工程研究与临床康复,2008,12(18):3 575-3 578.
[16]DENG Jia-gang,LI Chun-yang,WANG Hai-lian,et al.Amygdalin mediates relieved atherosclerosis in apolipoprotein E deficient mice through the induction of regulatory T cells[J].Biochemical and Biophysical Research Communications,2011,411(3):523-529.
[17]ZHANG Xiang-qun,HU Jian-gong,ZHUO Yu-zhen,et al.Amygdalin improves microcirculatory disturbance and attenuates pancreatic fibrosis by regulating the expression of endothelin-1and calcitonin gene-related peptide in rats[J].Journal of the Chinese Medical Association,2018,81(5):437-443.
[18]赵静,段杨峰,孔繁渊,等.超声波辅助提取枇杷核苦杏仁苷工艺优化[J].食品科学,2011,32(8):37-42.
[19]邬晓勇,孙雁霞,何钢,等.欧李种仁中苦杏仁苷的提取及其抗氧化活性[J].湖北农业科学,2013,52(19):4 764-4 767.
[20]刘进波,肖可,张天其,等.HPLC对比不同炮制方法对苦杏仁药材含量的影响[J].内蒙古中医药,2014(20):78.
[21]谢朝晖,李鑫,李文博,等.超声波法提取山杏仁中苦杏仁苷的单因素试验研究[J].河南城建学院学报,2012,21(2):31-37.
[22]YUE Kai, YE Meng, ZHOU Zu-ji,et al.The genus Cordyceps:a chemical and pharmacological review[J].Journal of Pharmacy&Pharmacology,2013,65(4):474-493.
[23]王涛,夏其乐,陆胜民.超声波提取杨梅疏果核仁中苦杏仁苷工艺研究[J].浙江农业学报,2014,26(3):779-785.
[24]马杰.桃仁苦杏仁苷的提取及其抑菌活性研究[J].食品工业,2015,36(5):116-120.
[25]范贤哲,李广凯,蒋艳杰,等.苦杏仁中苦杏仁苷提取工艺研究[J].广州化工,2018,46(10):81-84.