摘要
目的:建立桃仁配方颗粒中苦杏仁苷的含量测定方法,对其测定结果进行不确定度评价。方法:采用高效液相色谱法测定桃仁配方颗粒中苦杏仁苷的含量,并对实验过程中各个因素对结果不确定度的贡献进行具体分析,得到在95%置信区间下测量结果的扩展不确定度及其合成不确定度。结果:桃仁配方颗粒中苦杏仁苷含量测定结果的合成不确定度为0. 83%,在95%置信区间下测量结果的扩展不确定度为2. 36mg/g,最终得其报告不确定度为(142. 17±2. 36) mg/g。结论:本文利用方法学验证和不确定度的相关理论进行全面评价,证明其含量测定结果的准确性和可靠性,为其他配方颗粒的质量评价提供参考。
Objective: To establish a method for the determination of amygdalin in formula granules of peach kernel,the measurement results are evaluated with uncertainty. Method: HPLC was used to determine the content of amygdalin in peach kernel formula granules.And the uncertainty introduced by various factors was evaluated clearly in the process of measurement. Finally,the uncertainty of synthesis was calculated and the uncertainty of the measurement results was given in the 95% confidence interval. Results: The synthetic uncertainty of amygdalin content in peach kernel formula granules was 0. 83%. In the 95% confidence interval,the extended uncertainty of the measurement results was 2. 36 mg/g. The report uncertainty of the measurement results was( 142. 17 ± 2. 36) mg/g. Conclusion: In this paper,methodology verification and uncertainty related theories are used for comprehensive evaluation,and it proves the accuracy and reliability of content determination of amygdalin in the formula granules results. Meanwhile,it provides references for quality evaluation of other formula particles.
引文
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