抱茎苦荬菜的化学成分研究
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摘要
目的研究中药抱茎苦荬菜的化学成分。方法采用大孔吸附树脂、聚酰胺色谱、硅胶柱色谱﹑Sephadex LH-20柱色谱﹑ODS柱色谱和制备液相等色谱手段进行分离纯化,根据化合物的理化性质和波谱学等方法鉴定其结构。结果从抱茎苦荬菜植物的70%乙醇提取物中分离得到8个化合物,分别鉴定为木犀草素-7-龙胆二糖苷、luteolin-7-O-β-D-glucopyranosyl-(1→2)-β-D-glucopyranoside、5,7-二羟基-4′-甲氧基-黄酮-7-芦丁糖苷、木犀草素、木犀草素-7-O-β-D-吡喃葡萄糖苷、木犀草素7-O-β-D-吡喃葡萄糖醛酸苷、芹菜素、7β-羟基-β-谷甾醇。结论木犀草素-7-龙胆二糖苷、木犀草素7-O-β-D-吡喃葡萄糖醛酸苷和7β-羟基-β-谷甾醇是从苦荬菜属首次分离得到的化合物。
Objective To research the chemical composition of one traditional Chinese medicine called Ixeris sonchifolia(Bge.) Hance.Methods Macroporous adsorption resin,polyamide column chromatography,silica column chromatography,sephadex LH-20 column chromatography,ODS column chromatography and preparative liquid chromatograph were used to separation and purification.Structure identification was conducted according to physical and chemical properties and spectroscopy of compounds.Results 8 compounds separated from the 70% ethanol extract of Ixeris sonchifolia(Bge.)Hance and were respectively reseda-7-diglucoside gentianae,luteolin-7-O-β-D-glucopyranosyl-(1 →2)-β-D-glucopyranoside,5,7-2 hydroxy-4′-methoxy-flavonoids-7-glycosidic rutin,luteolin,mignonette element-7-O-β-D-pyran glycosidase,mignonette,7-O-β-D-pyran glucuronic acid glycosides,apigenin and 7β-hydroxy-beta sitosterol.Conclusion Compounds reseda-7-diglucoside gentianae,mignonette,7-O-β-D-pyran glucuronic acid glycosides and 7β-hydroxy-beta sitosterol were the first isolation from Ixeris genus.
Objective To research the chemical composition of one traditional Chinese medicine called Ixeris sonchifolia(Bge.) Hance.Methods Macroporous adsorption resin,polyamide column chromatography,silica column chromatography,sephadex LH-20 column chromatography,ODS column chromatography and preparative liquid chromatograph were used to separation and purification.Structure identification was conducted according to physical and chemical properties and spectroscopy of compounds.Results 8 compounds separated from the 70% ethanol extract of Ixeris sonchifolia(Bge.)Hance and were respectively reseda-7-diglucoside gentianae,luteolin-7-O-β-D-glucopyranosyl-(1 →2)-β-D-glucopyranoside,5,7-2 hydroxy-4′-methoxy-flavonoids-7-glycosidic rutin,luteolin,mignonette element-7-O-β-D-pyran glycosidase,mignonette,7-O-β-D-pyran glucuronic acid glycosides,apigenin and 7β-hydroxy-beta sitosterol.Conclusion Compounds reseda-7-diglucoside gentianae,mignonette,7-O-β-D-pyran glucuronic acid glycosides and 7β-hydroxy-beta sitosterol were the first isolation from Ixeris genus.
引文
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[18]杨燕,王洪庆,陈若芸.桑叶中的黄酮类化合物[J].药学学报,2010,45(1):77-81.
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[20]Markham KR,Ternal B,Stanley R,et al.Carbon-13 NMRstudies of flavonoids-Ⅲ:naturally occurring flavonoid glycosides and their acylated derivatives[J].Tetrahedron,1978,34(9):1389-1397.
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[22]靳鑫,时圣明,张东方,等.穿心莲化学成分的研究[J].中草药,2012,43(1):47-50.
[23]彭金咏,范国荣,吴玉田.白花败酱草化学成分研究[J].中药材,2005,(10):24-25.
[2]袁汀,刘蕊,黄晓玲.苦碟子研究进展[J].实用药物与临床,2004,7(4):44.
[3]叶冠,范明松,黄成钢,等.抱茎苦荬菜化学成分研究[J].中国药学杂志,2005,40(21):1613-1615.
[4]孟宪贞,倪素芳,索鸿勋.苦碟子化学成分的研究1.扩冠有效成分的分离和结构鉴定[J].中草药,1981,12(12):4.
[5]杨湛,李新莉,石凌钢,等.苦碟子中的木质素和酚酸类成分研究[J].齐齐哈尔大学学报,2012,28(1):52-54.
[6]齐晓丽,孟祥颖,王淑萍,等.苦碟子挥发油化学成分的分析[J].分子科学学报,2006,22(2):138-140.
[7]赵艳威,谢文利,于晓风,等.苦碟子皂苷对高脂血症大鼠血脂代谢的影响及其抗氧化作用[J].武警医学院学报,2005,14(3):165-168.
[8]贾德武,罗胜勇,叶寿山,等.苦碟子总黄酮对脑缺血性损伤保护作用的实验研究[J].中国临床药理学与治疗学,2011,16(6):616-620.
[9]陈春光,贾洪丽,吕首旭,等.苦碟子注射液对大鼠急性脑缺血-再灌注损伤的保护作用[J].中国临床药理学杂志,2012,28(3):196-197,199.
[10]李艳妍,尹一子,雎大员,等.碟脉灵注射液对高脂血症大鼠代谢的影响[J].吉林大学学报(医学版),2002,28(4):357-359.
[11]翟玉荣,黄燮南,于小凤,等.注射用苦碟子对心肌缺血再灌注损伤大鼠心肌酶和血黏度的影响[J].中药药理与临床,2008,24(2):53-55.
[12]郭科男,钱宁,董小君,等.苦碟子注射液对乙醇所致大鼠急性肝损伤的影响[J].时珍国医国药,2009,20(3):543-544.
[13]赵菊花,吴曙光,巫全胜,等.苦碟子注射液治疗贵州小型猪肝纤维化的实验研究[J].时珍国医国药,2010,21(3):586-588.
[14]张红霞,刘飞,葛勤利.苦碟子注射液抗大鼠肝纤维化的实验研究[J].临床军医杂志,2011,39(6):1052-1054.
[15]王建国,姜哲,谢遵江.苦碟子对恶性黑色素瘤细胞抑制及凋亡蛋白Bcl-2/Bax表达的影响[J].解剖科学进展,2013,19(2):148-152.
[16]周晓棉,郑洪浩,曹春阳,等.苦碟子注射液的抗肿瘤作用[J].沈阳药科大学学报,2007,24(2):103-108.
[17]柳晓琳,何雅慧,金艳书,等.苦碟子抗呼吸道病毒的实验研究[J].中国现代中药,2006,8(1):11-15.
[18]杨燕,王洪庆,陈若芸.桑叶中的黄酮类化合物[J].药学学报,2010,45(1):77-81.
[19]王立波,森川敏生,高慧媛,等.沙生蜡菊花中黄酮类成分的分离与鉴定[J].沈阳药科大学学报,2009,26(10):792-795.
[20]Markham KR,Ternal B,Stanley R,et al.Carbon-13 NMRstudies of flavonoids-Ⅲ:naturally occurring flavonoid glycosides and their acylated derivatives[J].Tetrahedron,1978,34(9):1389-1397.
[21]Ternai B,Markham KR.Carbon-13 NMR studies of flavonoids-Ⅰ:flavones and flavonols[J].Tetrahedron,1976,32(5):565-569.
[22]靳鑫,时圣明,张东方,等.穿心莲化学成分的研究[J].中草药,2012,43(1):47-50.
[23]彭金咏,范国荣,吴玉田.白花败酱草化学成分研究[J].中药材,2005,(10):24-25.