硫酸酯化苹果多糖对自由基清除作用的研究
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摘要
以N,N-二甲基甲酰胺(DMF)介质为反应溶剂,以氨基磺酸为酯化剂,利用超声波辅助法对苹果多糖进行硫酸酯化。采用DEAE-52离子柱层析对酯化后的多糖进行分离纯化,研究酯化前后苹果多糖对DPPH自由基、羟自由基(·OH)和超氧阴离子自由基(O_2~-·)的清除能力。结果表明,硫酸酯化对苹果多糖的自由基清除能力有提高作用,纯化后的多糖硫酸基团含量增多,自由基清除能力增强。
The apple soluble polysaccharides was sulfated using ultrasonic-assisted amino acid method with N,N-dimethylformamide medium for reaction solvent, and the amido-sulfonic acid for esterifying agency as well. DEAE-52 ion-exchange column chromatography was used to isolate the apple soluble polysaccharides. Antioxidant activities of the crude and purified sulfated polysaccharide were evaluated through their capability of scavenging DPPH, ·OH and O_2~-·radicals. The result of antioxidant activities showed that after the polysaccharides of sulfated had influence on the free radical scavenging activities. Purified polysaccharide sulfate groups in larger, stronger radical scavenging capacity.
The apple soluble polysaccharides was sulfated using ultrasonic-assisted amino acid method with N,N-dimethylformamide medium for reaction solvent, and the amido-sulfonic acid for esterifying agency as well. DEAE-52 ion-exchange column chromatography was used to isolate the apple soluble polysaccharides. Antioxidant activities of the crude and purified sulfated polysaccharide were evaluated through their capability of scavenging DPPH, ·OH and O_2~-·radicals. The result of antioxidant activities showed that after the polysaccharides of sulfated had influence on the free radical scavenging activities. Purified polysaccharide sulfate groups in larger, stronger radical scavenging capacity.
引文
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[15]Jing Wang,Quanbin Zhang,Zhongshan Zhang,et al.Synthesized phosphorylated and aminated derivatives of fucoidan and their potential antioxidant activity in vitro[J].International Journal of Biological Macromolecules,2009,44(2):170-174
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[17]SC Chang,B Y Hsu,B H Chen.Structural characterization of polysaccharides from Zizyphus jujuba and evaluation of antioxidant activity[J].International Journal of Biological Macromolecules,2010,(4):445-453
[18]Chen Y,Zhang H,Wang Y,et al.Sulfated modification of the polysaccharides from Ganoderma atrum and their antioxidant and immunomodulating activities[J].Food Chemistry,2015,186:231-238
[2]付成程,郭玉蓉,刘艳芳,等.苹果多糖的制备方法及防癌功能研究进展[J].农产品加工(学刊),2012,(02):100-102
[3]赵明慧,吕春茂,孟宪军,等.苹果渣水溶性膳食纤维提取及其对自由基的清除作用[J].食品科学,2013,22:75-80
[4]王振斌,刘加友,马海乐,等.无花果多糖提取工艺优化及其超声波改性[J].农业工程学报,2014,(10):262-269
[5]徐瑶,陈雪峰,刘宁.超声波辅助苹果水溶性膳食纤维硫酸酯化[J].食品与发酵工业,2015,(1):93-96
[6]贾琳斐.海红果多糖的提取分离、结构分析及体外抗氧化活性研究[D].西安:陕西师范大学,2014
[7]王莹,张海珠,刘熙,等.云南松松塔多糖含量测定2种方法的比较[J].食品研究与开发,2015,19:145-147
[8]董娜,贾艳菊,张晓,等.考马斯亮蓝法测定乳粉真蛋白的研究[J].食品科技,2011,(11):272-274
[9]陈乾,马天翔,郭宏举,等.硫酸钡-比浊法测定褐藻糖胶中硫酸根的含量[J].药学实践杂志,2012,30(2):118-120
[10]颜军,易勇,邬晓勇,等.黄芪多糖的相对分子量测定及单糖组成分析[J].食品科技,2012,(12):278-283
[11]杜柯,孙润广,赵凯,等.柴胡多糖的结构和抗氧化活性分析[J].生物加工过程,2011,(04):45-48
[12]卢茳虹,林宗毅,崔春,等.响应面优化柠檬酸提取海带抗氧化多糖的研究[J].食品工业科技,2012,33(15):22
[13]胡飞,何艳克.苦瓜多糖的分离纯化及抗氧化性的研究[J].食品工业科技,2009,(08):247-249
[14]朱振元,原静,李盛峰,等.古尼虫草多糖硫酸酯化修饰及其抗氧化活性[J].现代食品科技,2011,(01):1-5
[15]Jing Wang,Quanbin Zhang,Zhongshan Zhang,et al.Synthesized phosphorylated and aminated derivatives of fucoidan and their potential antioxidant activity in vitro[J].International Journal of Biological Macromolecules,2009,44(2):170-174
[16]Li S,Shah N P.Antioxidant and antibacterial activities of sulphated polysaccharides from Pleurotus eryngii and Streptococcus thermophilus ASCC 1275[J].Food Chemistry,2014,165:262-270
[17]SC Chang,B Y Hsu,B H Chen.Structural characterization of polysaccharides from Zizyphus jujuba and evaluation of antioxidant activity[J].International Journal of Biological Macromolecules,2010,(4):445-453
[18]Chen Y,Zhang H,Wang Y,et al.Sulfated modification of the polysaccharides from Ganoderma atrum and their antioxidant and immunomodulating activities[J].Food Chemistry,2015,186:231-238