摘要
苹果病毒病的发生严重影响了我国苹果产业的发展,培育脱毒苹果苗木非常重要。为了解苹果砧木组培苗的病毒病携带情况,以课题组保存的13个苹果砧木组培苗为材料,使用RT-PCR检测脱毒处理后样品中的苹果褪绿叶斑病毒(ACLSV)、苹果茎沟病毒(ASGV)、苹果茎痘病毒(ASPV)、苹果锈果类病毒(ASSVd)。结果显示,BP、W8N13、唐木田含有ASGV;HCT-2、BP、W8N13含有ACLSV;ME-2含有Apscaviroid;所检样品均未检出ASPV。试验获得5份无病毒苹果砧木组培苗。
The occurrence of apple virus disease seriously affects the development of apple industry in our country. In order to understand the virus carrying status of apple rootstock tissue culture seedlings, the tissue culture seedlings of apple rootstock preserved by our research group were used as materials. RT-PCR method was used to detect virus in virus-free samples. The results show that BP, W8 N13, Tangmutian contains ASGV;HCT-2, BP, and W8 N13 contain ACLSV; ME-2 contains Apscaviandroid; ASPV was not detected in any of the samples examined. In this test, 5 kinds of virus-free apple rootstocks were obtained.
引文
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